Role of human papillomavirus in the development of urothelial carcinoma

Abstract

It has been estimated that almost 10% of the worldwide cancer burden is linked to human papillomavirus (HPV) infection. Although the association between HPV and bladder carcinoma has been extensively investigated, data on the role of HPV in bladder carcinogenesis are controversial. The aim of the study was to assess the possible role of human papillomavirus in the development of urothelial bladder carcinomas. Formalin-fixed and paraffin-embedded archival tissue samples were used for DNA extraction. Seventy urothelial bladder carcinoma tissues were screened by nested-polymerase chain reaction (PCR) for HPV DNA with a control group of total 18 cervical tissues with invasive cervical carcinoma and cervical intraepithelial neoplasia III (CIN III). In the study group, we did not find HPV DNA positivity in any of the urothelial carcinomas. In the control group, 15 out of 18 (83.3%) samples were positive for the HPV DNA. These results indicated that there was no association between HPV infection and urothelial carcinomas.

Human papillomavirus detection in Moroccan patients with bladder cancer

“…These findings were very similar to those obtained from studies conducted in Egypt [19,28] and Italy [32]. In contrast, other studies reported no detection of HPV [7,26,33]. Several explanations of this variability have been proposed, including sampling problems, contamination, sensitivity of the detection systems, and geographic variation [34].…”

“…HPV is associated with more human cancers than any other virus, and it can be considered as one of the most important risk factors for human cancer [26]. It is well established that HPV shows a particular tropism for the epithelium of different mucosal sites, mainly of the oral and ano-genital regions.…”

Urothelial carcinoma with prominent squamous differentiation in the setting of neurogenic bladder: role of human papillomavirus infection

“…Reported HPV rates also vary widely for invasive urothelial carcinomas, ranging from 0 to 100%. [38][39][40] This variation may be due to the different methods of detection used in the studies. For frozen or fresh tissue, PCR is considered to be the gold standard, 41 although PCR technique is frequently blamed for being oversensitive, subject to contamination, and false-positive results.…”

“…1 HPV is a small circular double-stranded DNA virus with more than 100 different types described to date. 2 The potential of the virus to cause malignant or benign lesions dictates its classification into low-risk (6,11,40,42, and so on) or high-risk (16,18,31,33,45,51, and so on) types. The prototypical model for carcinogenesis is squamous cell carcinoma of the uterine cervix, in which nearly all such carcinomas are associated with HPV infection.…”

High-Risk Human Papillomavirus DNA Detected in Primary Squamous Cell Carcinoma of Urinary Bladder

“…The potential role that HPV may play in the development of bladder carcinomas has been previously studied in a few small series, but results of these studies [14][15][16][17][18][19][20][21] have been conflicting and no large-scale series or series based in the United States has been conducted to our knowledge. Additionally, most previous studies include only a small subset, if any, of pure SCC of urinary bladder.…”