Investigation of Antiproliferative Effect of Ether and Ethanol Extracts of Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Bull.:Fr.) P. Karst. (Higher Basidiomycetes) In Vitro Grown Mycelium

Abstract

This paper describes the study conducted to evaluate the antiproliferative activity of ether and ethanol extracts isolated from Piptoporus betulinus against cancer-derived cells. The fungal material used for extract preparation and further experiments was obtained from in vitro grown strains of P. betulinus. To the best of the authors’ knowledge, this is the first study evaluating antiproliferative potential of in vitro cultured birch polypore fungus. The effect of ether and ethanol extracts on cell proliferation, viability, and adhesion was assessed on colorectal adenocarcinoma cancer cell line LS180, whereas the cytotoxicity effect was investigated in normal colon epithelium-derived cell line CCD 841 CoTr. Studied extracts highly decreased the viability of cancer cells, slightly inhibiting proliferation and tumor cell adhesion in a time- and dose-dependent manner. Cytotoxicity of extracts against cells of normal colon epithelium origin was observed only at the highest studied concentration. The obtained results may seem interesting in comparison with previous studies on water extracts from natural grown P. betulinus. Future research on mycelial extract activity, as well as the content analysis, is needed.

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

“…The chemical composition of biomass extracts from mycelial cultures of F. betulina has so far only been studied to a limited extent. However, the anticancer properties of the extracts have been proven [ 8 ].…”

In vitroimmunomodulatory activity, cytotoxicity and chemistry of some central European polypores

“…Many formulations are based on a simple ethanol extract. Ethanol-extractable constituents such as triterpenoids and flavonoids or their mixtures are often reported to have potent antioxidant (Macakova et al 2010), anti-inflammatory (Yoshikawa et al 2005), and immunomodulatory (Quang et al 2006;Cyranka et al 2011) activities.…”

“…Complex mixtures may influence all these processes to different extents. The extracts of P. betulinus are rich in cytotoxic, antimicrobial and anti-inflammatory triterpenoids, lanostanes, and their esters (Lemieszek et al 2009;Cyranka et al 2011;Grienke et al 2014) that might be responsible for mild toxicity against HT-29 cell line. Polyporus squamosus is edible when young and soft.…”

Cultivation and utility of Piptoporus betulinus fruiting bodies as a source of anticancer agents

“…To date, for various research uses, P. betulinus mycelium has been cultivated in submerged and surface cultures (Cyranka et al 2011), while the fruiting bodies were obtained only from nature. Production of the fungus fruiting bodies has been described in only one paper.…”

“…As shown by pharmacological studies, the Birch polypore can also be widely used in modern medicine. The extracts of P. betulinus exhibited various biological activities, mainly cytotoxic and anti-proliferative, against cancer cells (Cyranka et al 2011; Kandefer-Szerszeń and Kawecki 1974; Lemieszek et al 2009). Many bioactive secondary metabolites, especially triterpenoids, were also isolated and identified (Grienke et al 2014; Wangun et al 2004).…”