Cultivation and utility of Piptoporus betulinus fruiting bodies as a source of anticancer agents

Pleszczyńska, Małgorzata; Wiater, Adrian; Siwulski, Marek; Lemieszek, Marta Kinga; Kunaszewska, Justyna; Kaczor, Józef; Rzeski, Wojciech; Janusz, Grzegorz; Szczodrak, Janusz

doi:10.1007/s11274-016-2114-4

Cited by 18 publications

(6 citation statements)

References 22 publications

(24 reference statements)

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“…Cyranka et al demonstrated that the type of mycelial extract has an effect on the growth and viability of colon adenocarcinoma cells [ 8 ]. Moreover Pleszczyńska et al showed the anti-proliferative and anti-immigration properties of water and ethanol extracts from fruiting bodies against human cancer cells [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

et al. 2018

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Fomitopsis betulina (Bull.) B.K. Cui, M.L. Han & Y.C. Dai has been used for medicinal purposes for over 5000 years. Numerous studies have confirmed the biological activity of compounds found in this species. The purpose of this study was a comparative analysis of selected groups of metabolites in the extracts from fruiting bodies and mycelial cultures. Phenolic acids (syringic, gallic, p-hydroxybenzoic, 3,4-dihydrophenylacetic), indole compounds (l-tryptophan, 5-hydroxy-l-tryptophan, 5-methyltryptamine), sterols (ergosterol, ergosterol peroxide, hexestrol, cholecalciferol), and triterpenes (betulinic acid, betulin) were determined quantitatively by high performance liquid chromatography with UV–Vis/DAD detection, while fatty acids were assessed with the gas chromatography method. Cytotoxic activity against selected human cancer cell lines was determined using the lactate dehydrogenase test. Anti-inflammatory activity was evaluated on lipopolysaccharide activated A549 cells. Those extracts with anti-inflammatory activity were evaluated for their inhibition of pro-inflammatory enzymes. The mycelium extract exhibited significant cytotoxic activity against prostate cancer cells, while the fruiting body extract indicated a moderate effect on the viability of melanoma and prostate cancer. Incubation of lung epithelial cells with biomass extract significantly decreased cyclooxygenase-2 levels compared to LPS activated A549 cells. This paper is the first report of a comparative quantitative analysis of the metabolites in mycelial cultures and fruiting bodies. In addition, a novel element of this study is its comparison of the cytotoxic and anti-inflammatory activity of the obtained extracts. The results of comparing the composition and activity of mycelium and fruiting bodies shows that the cultures could be proposed as a potential biotechnological source for selected biologically active compounds.

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Section: Resultsmentioning

confidence: 99%

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

et al. 2018

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“…The ratio of log yield was estimated at 2.8–6.1%. The only report on indoor production of F. betulina fruiting bodies was given by Pleszczyńska et al (2016). In the study, four strains of F. betulina isolated from natural habitats were applied.…”

Section: Cultivationmentioning

confidence: 99%

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

Pleszczyńska

Lemieszek

Siwulski

et al. 2017

World J Microbiol Biotechnol

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Higher Basidiomycota have been used in natural medicine throughout the world for centuries. One of such fungi is Fomitopsis betulina (formerly Piptoporus betulinus), which causes brown rot of birch wood. Annual white to brownish fruiting bodies of the species can be found on trees in the northern hemisphere but F. betulina can also be cultured as a mycelium and fruiting body. The fungus has a long tradition of being applied in folk medicine as an antimicrobial, anticancer, and anti-inflammatory agent. Probably due to the curative properties, pieces of its fruiting body were carried by Ötzi the Iceman. Modern research confirms the health-promoting benefits of F. betulina. Pharmacological studies have provided evidence supporting the antibacterial, anti-parasitic, antiviral, anti-inflammatory, anticancer, neuroprotective, and immunomodulating activities of F. betulina preparations. Biologically active compounds such as triterpenoids have been isolated. The mushroom is also a reservoir of valuable enzymes and other substances such as cell wall (1→3)-α-d-glucan which can be used for induction of microbial enzymes degrading cariogenic dental biofilm. In conclusion, F. betulina can be considered as a promising source for the development of new products for healthcare and other biotechnological uses.

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“…The most promising ones were even further tested through clinical trials and polysaccharide K, known also as krestin or PSK, from Coriolus versicolor , has been officially used in Japan as an adjuvant for cancer treatment [ 16 , 17 ]. The cultivation of polypore species is challenging as in the case of F. betulina (Chaga) [ 18 , 19 ]. If not, collecting the most promising species from the wild can be properly arranged.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans

Kozarski,

Klaus,

Špirović-Trifunović

et al. 2024

Molecules

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Growing mushrooms means meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Customized strains adapted to local conditions are urgently needed. Before introducing new species to the strain development pipeline, the chemical characterization and biological activity of wild ones need to be assessed. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated including: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and anti-angiotensin converting enzyme (ACE). The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to normal cells. The evaluated species provided a solid foundation for commercial development while keeping local ecology in mind.

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Cultivation and utility of Piptoporus betulinus fruiting bodies as a source of anticancer agents

Cited by 18 publications

References 22 publications

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans

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