Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats

Abstract

The current study aimed to investigate the protective effects of moderate aerobic exercise against chlorpyrifos (CPF)‐induced testes dysfunction. In excremental study, 48 adult male albino rats were randomly allocated into 16 groups of 3 rats each. Twelve experimental groups received intraperitoneal injection (5 days a week) of either 1.0 or 3.0 mg/kg body weight CPF in DMSO for 2, 4 or 6 consecutive weeks. Seven of these experimental groups were subjected to run at moderate exercise intensity for 5 days per week over 2 weeks, whereas the other groups were not. Two groups (sham groups) were administered to the equal volume of vehicle (DMSO) for 4 or 6 consecutive weeks. The remaining two groups comprised the control groups including a sedentary and an exercise‐trained control group. Exercise training leads to a markedly increase in testicular superoxide dismutase (SOD) activity in CPF‐exposed rats compared with corresponding sedentary animals (p < .05). Lipid peroxidation level was found to be significantly decreased in the testis of exercised animals that had been exposed to CPF (p < .05). Our results suggest that aerobic exercise can alleviate the oxidative stress induced by sub‐acute CPF exposure in testis. Exercise training could barely mitigate CPF‐induced testicular damages in rats.

Antioxidant Effects of Aerobic Training and Crocin Consumption on Doxorubicin-Induced Testicular Toxicity in Rats

“…The study by Nikbin et al also showed that aerobic activity in chlorpyrifos (CPF)-injected rats increased SOD activity in two weeks of activity, but in the fourth and sixth weeks, SOD activity decreased in the CPF-injected groups or groups without aerobic activity. Also, MDA-induced lipid peroxidation in testicular tissue improved with training in the CPF-injected group, which was not statistically significant ( 32 ). Overall, the results of this study, as in the present study, did not show significant improvement for most CPF-induced injuries in trained rats.…”

“…The interactive effects of HIIT and Crocin consumption on oxidant / antioxidant redox balance have also been investigated in the heart and liver tissue of DOX-treated rats, indicating favorable interactions on decreasing MDA and increasing CAT and SOD ( 32 , 33 ). This study showed for the first time that concurrent Crocin supplementation, especially at a dose of 50 mg/kg/d and HIIT training, with a synergistic effect, improves antioxidant defense indicators and reduces oxidative pressure in the testicular tissue of rats.…”