Intrinsic extracellular matrix properties regulate stem cell differentiation

Reilly, Gwendolen C.; Engler, Adam J.

doi:10.1016/j.jbiomech.2009.09.009

Cited by 694 publications

(536 citation statements)

References 75 publications

(94 reference statements)

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“…44 These cues are provided by the extracellular matrix of the native niche in which the stem cell resides. 45 Among these cues, matrix stiffness is a critical parameter that governs the fate of stem cell differentiation. 35 To assess differentiation, cell circularity and the cell spreading area provides a good estimate of the morphology of cells.…”

Section: Discussionmentioning

confidence: 99%

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

et al. 2016

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We report a new class of amyloid-inspired peptide hydrogels that was designed and based on α-synuclein protein for which hydrogel formation is triggered by various stimuli, such as heating/cooling or changes in pH. The peptides resemble a cross-β-sheet-rich amyloid, and they assemble into a nanofibrous meshwork that mimics the natural extracellular matrix. Our design principle allows easy manipulation of the gelator sequence to exploit the desirable properties of amyloids for use in cell replacement therapies for neurodegenerative diseases. The amyloid hydrogels facilitate the attachment and neuronal differentiation of mesenchymal stem cells (MSCs) and assist in the delivery and engraftment of MSCs in the substantia nigra and caudate putamen of a Parkinsonian mouse model.

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Section: Discussionmentioning

confidence: 99%

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

et al. 2016

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“…The evolving intrinsic properties of the extracellular matrix may also play a role in driving this differentiation pathway [64]. Furthermore, the subcutaneous environment is well vascularised, which differs from the low oxygen microenvironment of articular cartilage.…”

Section: Discussionmentioning

confidence: 99%

Engineering osteochondral constructs through spatial regulation of endochondral ossification

et al. 2013

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Chondrogenically primed bone marrow derived mesenchymal stem cells (MSCs) have been shown to become hypertrophic and undergo endochondral ossification when implanted in vivo. Modulating this endochondral phenotype may be an attractive approach to engineering the osseous phase of an osteochondral implant. The objective of this study was to engineer an osteochondral tissue by promoting endochondral ossification in one layer of a bi-layered construct and stable cartilage in the other. The top-half of bi-layered agarose hydrogels were seeded with culture expanded chondrocytes (termed chondral layer) and the bottom half of the bi-layered agarose hydrogels with MSCs (termed osseous layer). Constructs were cultured in a chondrogenic medium for 21 days and thereafter were either maintained in a chondrogenic medium, transferred to a hypertrophic medium, or implanted subcutaneously into nude mice. This structured chondrogenic bi-layered co-culture was found to enhance chondrogenesis in the chondral layer, appearing to help re-establish the chondrogenic phenotype that is lost in chondrocytes during monolayer expansion. Furthermore, the bilayered co-culture appeared to suppress hypertrophy and mineralisation in the osseous layer.The addition of hypertrophic factors to the media was found to induce mineralisation of the osseous layer in vitro. A similar result was observed in vivo where endochondral ossification was restricted to the osseous layer of the construct leading to the development of an osteochondral tissue. This novel approach represents a potential new treatment strategy for the repair of osteochondral defects.

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“…However, with regard to the 22 rigidity of scaffold, it has been suggested that stem cells become osteoblasts-like when cultured on stiff matrix (34 kPa hydrogels), while they become neuron-like, myocyte-like on soft ones (Reilly and Engler 2010). Thus, higher concentration of agarose gel (more rigid) with reasonable hydraulic permeability (nutrition and waste exchange) may be better to create similar microenvironment in vitro for bone tissue regeneration.…”

Section: Scaffold Choosingmentioning

confidence: 99%

The effect of mechanical loading on osteogenesis of human dental pulp stromal cells in a novel in vitro model

Sun

Wang

et al. 2014

Cell Tissue Res

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Tooth loss often results the alveolar bone resorption due to lack of mechanical stimulation. Thus, the mechanism of mechanical loading on stem cells osteogenesis is crucial for alveolar bone regeneration. This project aims to investigate the effect of mechanical loading on human dental pulp stromal cells (hDPSCs) osteogenesis in a novel in vitro model. Briefly, 1×10 7 hDPSCs were seeded into 1 mL 3% agarose gel in a 48-well-plate. Then a loading tube was placed in the middle of the gel to mimicking the tooth chewing movement

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Intrinsic extracellular matrix properties regulate stem cell differentiation

Cited by 694 publications

References 75 publications

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

Engineering osteochondral constructs through spatial regulation of endochondral ossification

The effect of mechanical loading on osteogenesis of human dental pulp stromal cells in a novel in vitro model

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