The effect of mechanical loading on osteogenesis of human dental pulp stromal cells in a novel in vitro model

Ji, Jun; Sun, Weibin; Wang, Wenmei; Munyombwe, Theresa; Yang, Xuebin

doi:10.1007/s00441-014-1907-8

Cited by 16 publications

(11 citation statements)

References 57 publications

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“…Other groups have successfully implanted hydroxyapatite/agarose hydrogels as bone graft material, proving osteoconductive properties of composites ( 26 , 28 ). Furthermore, the osteogenic differentiation of dental stromal cells under mechanical stimulation in agarose gels has been recently described ( 29 ). The ASNIS-III s screw was set in the centre of the 3D osteoblast-agarose scaffold to expose the bone cells to the EMF + EF.…”

Section: Discussionmentioning

confidence: 99%

Magnetically induced electrostimulation of human osteoblasts results in enhanced cell viability and osteogenic differentiation

Hiemer

Ziebart

Jonitz‐Heincke

et al. 2016

International Journal of Molecular Medicine

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The application of electromagnetic fields to support the bone-healing processes is a therapeutic approach for patients with musculoskeletal disorders. The ASNIS-III s-series screw is a bone stimulation system providing electromagnetic stimulation; however, its influence on human osteoblasts (hOBs) has not been extensively investigated. Therefore, in the present study, the impact of this system on the viability and differentiation of hOBs was examined. We used the ASNIS-III s screw system in terms of a specific experimental test set-up. The ASNIS-III s screw system was used for the application of electromagnetic fields (EMF, 3 mT, 20 Hz) and electromagnetic fields combined with an additional alternating electric field (EMF + EF) (3 mT, 20 Hz, 700 mV). The stimulation of primary hOBs was conducted 3 times per day for 45 min over a period of 72 h. Unstimulated cells served as the controls. Subsequently, the viability, the gene expression of differentiation markers and pro-collagen type 1 synthesis of the stimulated osteoblasts and corresponding controls were investigated. The application of both EMF and EMF + EF using the ASNIS-III s screw system revealed a positive influence on bone cell viability and moderately increased the synthesis of pro-collagen type 1 compared to the unstimulated controls. Stimulation with EMF resulted in a slightly enhanced gene expression of type 1 collagen and osteocalcin; however, stimulation with EMF + EF resulted in a significant increase in alkaline phosphatase (1.4-fold) and osteocalcin (1.6-fold) levels, and a notable increase in the levels of runt-related transcription factor 2 (RUNX-2; 1.54-fold). Our findings demonstrate that stimulation with electromagnetic fields and an additional alternating electric field has a positive influence on hOBs as regards cell viability and the expression of osteoblastic differentiation markers.

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Section: Discussionmentioning

confidence: 99%

Magnetically induced electrostimulation of human osteoblasts results in enhanced cell viability and osteogenic differentiation

Hiemer

Ziebart

Jonitz‐Heincke

et al. 2016

International Journal of Molecular Medicine

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“…Furthermore, using a bioreactor that mimicked biting force, Ji et al (2014) successfully developed a novel method to enhance the osteogenic differentiation of DPSCs. They showed that the application of mechanical forces that mimicked the dynamics of those exerted in vivo on DPSCs could be used to promote bone formation and limit bone resorption ( Ji et al, 2014 ).…”

Section: Mechanical Stimuli That Induce Osteogenic Differentiation Ofmentioning

confidence: 99%

“…Several investigations have also shown that mechanical stimuli including dynamic hydrostatic pressure, cyclic tensile strain, mechanical compression and cyclic uniaxial compressive strain can promote the odontogenic differentiation of DPSCs ( Yu et al, 2009 ; Lee et al, 2010 ; Miyashita et al, 2017 ; Yang et al, 2017 ). While other studies have demonstrated that cyclic mechanical tension, pulsating fluid flow, surface topographies, equiaxial static tensile strain and mechanical loading can promote the osteogenic differentiation of DPSCs ( Han et al, 2008 , 2010 ; Kraft et al, 2010 , 2011 ; Ji et al, 2014 ; Kolind et al, 2014 ; Tabatabaei et al, 2014 ). Interestingly, mechanical forces such as uniaxial stretch can increase the proliferation of DPSCs while inhibiting the odontogenic and osteogenic differentiation of DPSCs, indicating that mechanical stimuli are therefore critical and contextually important in modifying DPSC fate ( Cai et al, 2011 ; Hata et al, 2012 ).…”

Section: Introductionmentioning

confidence: 96%

“…In this context, several studies have shown that mechanical stimuli including cyclic mechanical tension, low-intensity pulsed ultrasound (LIPUS), uniaxial mechanical stretch and cyclic uniaxial compressive stress are able to induce the proliferation of DPSCs ( Han et al, 2008 ; Hata et al, 2012 ; Gao et al, 2016 , 2017 ; Yang et al, 2017 ). Furthermore physical stimuli such as loading, surface topographies, dynamic hydrostatic pressure and pulsating fluid flow can reportedly promote the differentiation of DPSCs (see Table 1 and Figure 1 ) ( Han et al, 2008 , 2010 ; Yu et al, 2009 ; Kraft et al, 2010 , 2011 ; Lee et al, 2010 ; Ji et al, 2014 ; Kolind et al, 2014 ; Tabatabaei et al, 2014 ; Miyashita et al, 2017 ; Yang et al, 2017 ). Several investigations have also shown that mechanical stimuli including dynamic hydrostatic pressure, cyclic tensile strain, mechanical compression and cyclic uniaxial compressive strain can promote the odontogenic differentiation of DPSCs ( Yu et al, 2009 ; Lee et al, 2010 ; Miyashita et al, 2017 ; Yang et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Dental Pulp Stem Cell Mechanoresponsiveness: Effects of Mechanical Stimuli on Dental Pulp Stem Cell Behavior

et al. 2018

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Dental pulp is known to be an accessible and important source of multipotent mesenchymal progenitor cells termed dental pulp stem cells (DPSCs). DPSCs can differentiate into odontoblast-like cells and maintain pulp homeostasis by the formation of new dentin which protects the underlying pulp. DPSCs similar to other mesenchymal stem cells (MSCs) reside in a niche, a complex microenvironment consisting of an extracellular matrix, other local cell types and biochemical stimuli that influence the decision between stem cell (SC) self-renewal and differentiation. In addition to biochemical factors, mechanical factors are increasingly recognized as key regulators in DPSC behavior and function. Thus, microenvironments can significantly influence the role and differentiation of DPSCs through a combination of factors which are biochemical, biomechanical and biophysical in nature. Under in vitro conditions, it has been shown that DPSCs are sensitive to different types of force, such as uniaxial mechanical stretch, cyclic tensile strain, pulsating fluid flow, low-intensity pulsed ultrasound as well as being responsive to biomechanical cues presented in the form of micro- and nano-scale surface topographies. To understand how DPSCs sense and respond to the mechanics of their microenvironments, it is essential to determine how these cells convert mechanical and physical stimuli into function, including lineage specification. This review therefore covers some aspects of DPSC mechanoresponsivity with an emphasis on the factors that influence their behavior. An in-depth understanding of the physical environment that influence DPSC fate is necessary to improve the outcome of their therapeutic application for tissue regeneration.

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“…This extends previous studies where mineralization and mature bone formation was found to be increased by mimicking vascularization in vitro in cocultures of endothelial cells and human MSC. [57][58][59][60] It is also important to consider that ADSCs have previously been shown to exhibit a proangiogenic effect via release of angiogenic factors such as vascular endothelial growth factor (VEGF), differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. [61][62][63] The data shown here further support the proangiogenic potential of these cells which then augments their osteogenic maturation capabilities.…”

Section: D Culture and Vascularization Promote Hadsc Osteogenesis mentioning

confidence: 99%

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

Ibrahim

Rodríguez-Flórez

Gardner

et al. 2020

Stem Cells Translational Medicine

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While human adipose-derived stem cells (hADSCs) are known to possess osteogenic differentiation potential, the bone tissues formed are generally considered rudimentary and immature compared with those made by bone-derived precursor cells such as human bone marrow-derived mesenchymal stem cells (hBMSCs) and less commonly studied human calvarium osteoprogenitor cells (hOPs). Traditional differentiation protocols have tended to focus on osteoinduction of hADSCs through the addition of osteogenic differentiation media or use of stimulatory bioactive scaffolds which have not resulted in mature bone formation. Here, we tested the hypothesis that by reproducing the physical as well as biochemical bone microenvironment through the use of three-dimensional (3D) culture and vascularization we could enhance osteogenic maturation in hADSCs. In addition to biomolecular characterization, we performed structural analysis through extracellular collagen alignment and mineral density in our bone tissue engineered samples to evaluate osteogenic maturation. We further compared bone formed by hADSCs, hBMSCs, and hOPs against mature human pediatric calvarial bone, yet not extensively investigated. Although bone generated by all three cell types was still less mature than native pediatric bone, a fibrin-based 3D microenvironment together with vascularization boosted osteogenic maturation of hADSC making it similar to that of bone-derived osteoprogenitors. This demonstrates the important role of vascularization and 3D culture in driving osteogenic maturation of cells easily available but constitutively less committed to this lineage and suggests a crucial avenue for recreating the bone microenvironment for tissue engineering of mature craniofacial bone tissues from pediatric hADSCs, as well as hBMSCs and hOPs.

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The effect of mechanical loading on osteogenesis of human dental pulp stromal cells in a novel in vitro model

Cited by 16 publications

References 57 publications

Magnetically induced electrostimulation of human osteoblasts results in enhanced cell viability and osteogenic differentiation

Magnetically induced electrostimulation of human osteoblasts results in enhanced cell viability and osteogenic differentiation

Dental Pulp Stem Cell Mechanoresponsiveness: Effects of Mechanical Stimuli on Dental Pulp Stem Cell Behavior

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

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