Intracloacal infection with avian infectious bronchitis virus

Cloacal Inoculation with the Connecticut Strain of Avian Infectious Bronchitis Virus: An Attempt to Produce Nephropathogenic Virus by in vivo Passage Using Cloacal Inoculation.

“…These results indicate that the strain A-5968, as well as the nephrosis-causing strain HS-91, is capable of inducing kidney lesions, only when the virus is inoculated via the cloaca. This conclusion is in line with a hypothesis put forward by Uenaka et al [ 13 ] that the cloaca is one of the most efficient natural inoculation routes to spread IBV to the kidneys. The cloacal inoculation of IBV has the ability to provide a larger amount of virus to the chick kidneys within a shorter time compared with tracheal inoculation.…”

“…SN antibody was produced more quickly following cloacal inoculation with the strain A-5968 than following tracheal inoculation, although chicks inoculated with the nephropathogenic IBV strain HS-91 via the cloaca showed similar SN antibody production to those inoculated via the trachea [13]. Toro et al [12] reported that the cloacal route can be a vaccination route for live IBV vaccines.…”

“…Marked antigenic variation did 1 2 1 2 inoculation [3]. On the other hand, the chicks inoculated with a nephrosis-causing IBV, strain HS-91, via the cloaca showed more severe clinical signs, grosser kidney lesions and higher mortality than those inoculated via the trachea [13]. However, investigations of cloacal inoculation with non-nephrosis-associated IBV strains have not so far been performed.…”

“…Experimental infections with strain A-5968 via the cloaca or trachea of SPF chicks Experiment 1: Experiment 1 (Expt 1) was basically performed as described previously [13]. Sixty 4-day-old chicks were divided into two groups, groups C and R and each group contained 30 chicks.…”

“…inoculation and more virus were recovered from kidneys in comparison to those inoculated via the trachea [13]. Thus it can be considered that cloacal inoculation is an effective route to reproduce nephrosis/nephritis with IBVs that have strong nephrotropism.…”