Intracellular precipitation of hydroxyapatite mineral and implications for pathologic calcification

Azari, Fereshteh; Vali, Hojatollah; Guerquin‐Kern, Jean‐Luc; Wu, Ting-Di; Croisy, Alain; Sears, S. Kelly; Tabrizian, Maryam; McKee, Marc D.

doi:10.1016/j.jsb.2008.03.003

Cited by 53 publications

(55 citation statements)

References 87 publications

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“…SIMS imaging was performed using a NanoSIMS-50 Ion Microprobe (CAMECA) operating in scanning mode (32,33). The elemental mapping was performed on the same thin section previously observed by TEM.…”

Section: Methodsmentioning

confidence: 99%

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Byrne

Ball²,

Guerquin‐Kern³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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107

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Intracellular magnetite crystal formation by magnetotactic bacteria has emerged as a powerful model for investigating the cellular and molecular mechanisms of biomineralization, a process common to all branches of life. Although magnetotactic bacteria are phylogenetically diverse and their crystals morphologically diverse, studies to date have focused on a few, closely related species with similar crystal habits. Here, we investigate the process of magnetite biomineralization in Desulfovibrio magneticus sp. RS-1, the only reported species of cultured magnetotactic bacteria that is outside of the α-Proteobacteria and that forms bulletshaped crystals. Using a variety of high-resolution imaging and analytical tools, we show that RS-1 cells form amorphous, noncrystalline granules containing iron and phosphorus before forming magnetite crystals. Using NanoSIMS (dynamic secondary ion mass spectroscopy), we show that the iron-phosphorus granules and the magnetite crystals are likely formed through separate cellular processes. Analysis of the cellular ultrastructure of RS-1 using cryo-ultramicrotomy, cryo-electron tomography, and tomography of ultrathin sections reveals that the magnetite crystals are not surrounded by membranes but that the iron-phosphorus granules are surrounded by membranous compartments. The varied cellular paths for the formation of these two minerals lead us to suggest that the iron-phosphorus granules constitute a distinct bacterial organelle.bacterial organelle | biomineralization | magnetotactic bacteria | dynamic secondary ion mass spectroscopy | magnetite B iomineralization, the biologically controlled transformation of inorganic compounds into highly-ordered structures, is performed by organisms in all branches of life, from bacteria to humans. Because biogenic minerals often have superior properties to their chemically synthesized counterparts, understanding biomineralization is important to fields as diverse as inorganic materials synthesis and medicine (1, 2). Among the numerous organisms capable of biomineralization, magnetotactic bacteria (MB), a group of microbes that form intracellular chains of magnetic minerals including magnetite and greigite, have become powerful models for studying biomineralization because of their relatively fast growth rate and genetic tractability (3).Investigation of the cellular ultrastructure of MB has shown that the magnetite crystals are formed within membranous intracellular compartments called magnetosomes, which are present before the formation of magnetite (4, 5). Other studies have shown that when magnetite formation is induced by decreasing oxygen levels or adding iron to iron-deprived cells, multiple crystals in the chain nucleate simultaneously (5, 6). These insights into the kinetics of magnetite biomineralization have been complemented by molecular studies. A genomic island called the magnetosome island (MAI) has been found in all MB studied to date. Loss of the MAI results in an absence of magnetosome membranes and magnetite crystals, and gene...

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Section: Methodsmentioning

confidence: 99%

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Byrne

Ball²,

Guerquin‐Kern³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

107

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“…Although calcification is mainly extracellular, intracellular calcification has been reported and may contribute to pathologic calcification of soft tissues (Azari et al, 2008). The kidneys could therefore be such a location (figure 2).…”

Section: +mentioning

confidence: 99%

“…This is mitigated by magnesium and fluoride, depending strictly on the dose. Azari et al (2008) experimentally found that hydroxyapatite crystals nucleate within intracellular membrane-bounded compartments. The discussion thus far emphasizes the extreme importance of the critical ratios of F⁻, Ca 2+ , Mg 2+ and Na + that activate chronic kidney diseases not related to diabetes, hypertension and any other known factors.…”

Section: +mentioning

confidence: 99%

Groundwater fluoride as a geochemical marker in the etiology of chronic kidney disease of unknown origin in Sri Lanka

Dissanayake

Chandrajith

2017

Ceylon J. Sci.

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“…Concerning cytocompatibility, melt-quenched bioglasses with a zinc content in the range 5-20 mol% showed significant cytotoxicity against human osteoblasts (Aina et al, 2007). In contrast, Balamurugan et al reported a minimal cytotoxicity with sol-gel glass composition of (mol%) 64% SiO 2 , 26% CaO, 5% P 2 O 5 , 5% ZnO towards murine osteoblasts (Balamurugan et al, 2007). In a companion paper, we presented evidence of good bioactivity and cytocompatibility for a 58S-based bioglass with the following composition: 59.7 mol% SiO 2 -36.2 mol% CaO -3.8 mol% P 2 O 5 -0.3 mol% (0.4 wt%) ZnO (58S-Zn0.4) (Bini et al, 2009).…”

Section: Introductionmentioning

confidence: 98%

“…On the other hand, it is important to check the possibility to extend the cytocompatibility range of ZnO, since this additive could positively modify the physico-chemical properties of the bioglass. The availability of more detailed cytotoxicity studies is also desirable because of the contradictory results reported in the literature (e.g., Aina et al, 2007;Balamurugan et al, 2007) which, however, may be dependent on many factors, including cell line, synthesis routes and related glass microstructure, surface morphology, zinc release kinetics, etc.…”

Section: Introductionmentioning

confidence: 99%

In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass

Saino

Grandi

Quartarone

et al. 2011

eCM

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Bioactive glasses synthesized by the sol-gel technique possess many of the qualities associated with an ideal scaffold material for a bone graft substitute. In view of the potential clinical applications, we performed a detailed in vitro study of the biological reactivity of synthesized 58S bioactive glass containing-zinc, in terms of osteoblast morphology, proliferation, and deposition of a mineralized extracellular matrix (ECM). Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the deposition of a calcified extracellular matrix by ELISA assay and quantitative RT-PCR (qRT-PCR). In comparison with pure silica and 58S, the 58S-Zn0.4 bioglass showed a significant increase in cellular proliferation and deposition of ECM components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, type-I and -III collagens. Calcium deposition was significantly higher than on pure silica and 58S samples. Also Alkaline phosphatase (ALP) activity and its protein content was higher with respect to pure silica and 58S. qRT-PCR analysis revealed the up-regulation of type-I collagen, bone sialoprotein and osteopontin genes. All together these results demonstrate the cytocompatibility of 58S-Zn0.4 bioglass and its capability to promote osteoblast differentiation.

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Intracellular precipitation of hydroxyapatite mineral and implications for pathologic calcification

Cited by 53 publications

References 87 publications

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Groundwater fluoride as a geochemical marker in the etiology of chronic kidney disease of unknown origin in Sri Lanka

In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass

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