In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass

Saino, Enrica; Grandi, S.; Quartarone, Eliana; Maliardi, Valentina; Galli, Daniela; Bloise, Nora; Fassina, Lorenzo; Angelis, Maria Gabriella Cusella De; Mustarelli, Piercarlo; Imbriani, Marcello; Visai, Livia

doi:10.22203/ecm.v021a05

Cited by 76 publications

(74 citation statements)

References 58 publications

(51 reference statements)

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“…36 The phenotype of the Saos-2 cell is considered to have a mature osteoblast phenotype because it reproduces many of the primary human 37 Saos-2 cells are also able to produce collagenous mineralized bone matrix containing type I and type V collagen and chondroitin sulfate proteoglycans. [38][39][40] Being from a cell line, Saos-2 cells are available for experiments in high numbers, and they are therefore ideal for studies of ECM deposition for which a hyperconfluent layer of cells is needed. In addition, the Saos-2 cell line is advantageous because of its phenotype stability during multiple passages.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

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Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings.

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Section: Discussionmentioning

confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

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“…It is thought to have antibacterial properties [300] and some studies report beneficial cellular response [301,302], but it can also cause toxicity [303].…”

Section: Antibacterial Propertiesmentioning

confidence: 99%

Reprint of: Review of bioactive glass: From Hench to hybrids

2015

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“…12 Zinc-containing BG have shown enhanced calcified matrix deposition and ALP activity compared with BG without zinc. 13 Moreover, Zn-containing BG scaffolds were shown to exhibit antibacterial activity while retaining biocompatibility with human osteoblast-like cells (HOS). 14 Zinc-containing BGN are therefore attractive materials for bone regeneration, as BGN can interact with cells at the nanoscale, thus delivering Zn ions more effectively and at more appropriate rates than conventional (mm sized) particles.…”

Section: Introductionmentioning

confidence: 99%

ZnO quantum dots modified bioactive glass nanoparticles with pH-sensitive release of Zn ions, fluorescence, antibacterial and osteogenic properties

et al. 2016

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a Zinc (Zn)-containing materials have osteogenic and antibacterial activities while bioactive glass nanoparticles (BGN) show bone-bonding ability, as well as osteoconductive and osteoinductive properties. Zn-containing BGN are therefore considered to be promising materials for various biomedical applications, particularly in bone regeneration. In this study, we report a convenient method to prepare Zn-containing BGN by coating ZnO quantum dots (QDs) on BGN via electrostatic interactions. The synthesized ZnO-BGN nanocomposite particles are spherical and highly dispersed, and exhibit a unique fluorescence behavior under UV excitation, emitting three wavelengths in the violet, blue and green range.ZnO-BGN showed apatite-forming ability upon immersion in simulated body fluid, but their apatite formation was delayed compared to BGN. Interestingly, ZnO-BGN showed a rapid release of Zn ions at pH 4 but a far slower release at pH 7.4. ZnO-BGN also exhibited antibacterial effects on both Gram-positive and Gram-negative bacteria at the concentrations of 1, 0.1, and 0.01 mg mL , but ZnO-BGN inhibited the relative proliferation of hMSC compared to BGN and the control according to the MTT assay. Notably ZnO-BGN improved the osteogenic differentiation of hMSC as indicated by the determination of the alkaline phosphatase activity. In conclusion, coating quantum dots on BGN is a promising strategy to produce Zn-containing BGN. The synthesized ZnO-BGN are potential materials for bone regeneration, considering their apatite-forming ability, unique ion-release behavior, effective antibacterial activity, non-cytotoxicity, and osteogenic potential.

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In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass

Cited by 76 publications

References 58 publications

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Reprint of: Review of bioactive glass: From Hench to hybrids

ZnO quantum dots modified bioactive glass nanoparticles with pH-sensitive release of Zn ions, fluorescence, antibacterial and osteogenic properties

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