Intra-specific composition and succession of Bifidobacterium longum in human feces

Kohara, Tomomi; Hayashi, Sachiko; Hamatsuka, Juri; Nishitani, Yosuke; Masuda, Yoshihiro; Osawa, Ro

doi:10.1016/j.syapm.2006.06.005

Cited by 9 publications

(12 citation statements)

References 10 publications

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“…do not colonize the human colon. A pulsed-field gel electrophoresis (PFGE) analysis of fecal B. longum isolates of 12 human subjects revealed that each subject harbored strains of unique clones and most of them had persisted in the host intestine for over 68 weeks (Kohara et al, 2006). In addition, PFGE profiles of these isolates were different from that of any B. longum commercially available.…”

Section: Discussionmentioning

confidence: 99%

Survival of Bifidobacterium animalis subsp. lactis OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

Watanabe

Isono

2012

FSTR

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The survival of Bifidobacterium animalis subsp. lactis OPB-1 in the gastrointestinal tract after its administration in a milk-free yogurt-like soybean product and effects on fecal microbiota in humans were determined. Twelve healthy volunteers were administered 100 g of yogurt-like soybean product for 3 weeks. The plate count method combined with specific PCR and quantitative real-time PCR were used to estimate the numbers of total Bifidobacterium and B. animalis subsp. lactis in fecal samples. B. animalis subsp. lactis was detected during the administration period but not before. The intake number of B. animalis subsp. lactis OPB-1 was 2.4 × 10 10 CFU/day by the plate count method and 4.2 × 10 13 copies/day by quantitative real-time PCR. However, the number of excreted B. animalis subsp. lactis per day was estimated to be more than 10 times the intake number, by both methods. The percentage of Bifidobacterium increased from 16.9 ± 21.8% to 34.1 ± 20.4% by the plate count method and 18.9 ± 13.4% to 27.8 ± 15.8% by the T-RFLP method after 3 weeks administration.

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Section: Discussionmentioning

confidence: 99%

Survival of Bifidobacterium animalis subsp. lactis OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

Watanabe

Isono

2012

FSTR

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“…Previous pulsed-field gel electrophoresis (PFGE) analysis of fecal isolates of B. longum from 12 human subjects (Kohara et al 2007) revealed that each harbored strains classified into several host-specific pulsotype groups, most of which had persisted in the hosts' intestine over 15 months. The experimental finding suggests that bifidobacteria have the ability to adhere to the intestinal wall in a host-specific manner.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism and distribution of putative cell-surface adhesin-encoding ORFs among human fecal isolates of Bifidobacterium longum subsp. longum

Iguchi

Umekawa

Maegawa

et al. 2010

Antonie van Leeuwenhoek

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The polymorphism of ORFs encoding putative cell-surface adhesins was investigated in Bifidobacterium longum subsp. longum. Firstly, we performed a PCR assay targeting 15 ORFs encoding putative adhesion proteins, which included 8 ORFs with a sortase targeting LPXTG motif, in 42 strains of different pulsotypes isolated from fecal samples from 12 human individuals. We found a variability in the presence of an ORF, BL0675, which encodes a putative fimbrial subunit protein. We sequenced ORFs corresponding to BL0675 in the 42 strains and adjacent ORFs corresponding to BL0674 and BL0676. The results indicated that ORFs corresponding to BL0675 were highly polymorphic with five variant types (i.e. A-, B-, C-, D-, and E-types). Meanwhile, BL0674 and BL0676, which encode an additional putative fimbrial subunit protein and a fimbrial-associated sortase-like protein, were highly conserved. Subsequent quantitative polymerase chain reaction (qPCR) assays targeting the variant types in 89 human fecal samples revealed that A-type was the most commonly distributed (74.2%), followed by B-type (59.6%), D-type (31.5%), E-type (32.6%) and C-type (5.6% prevalence). Since BL0675 is considered to be a fimbrial protein with glycoprotein-binding ability, the proteins encoded by the five variant types of BL0675 may have specific binding properties to various carbohydrate structures expressed on the human intestinal wall, thereby allowing B. longum to colonize the intestine in a host-specific manner.

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“…However, opinions vary, and understanding of the main factors exerting an impact on the diversity of the intestinal inhabitants at strain level is currently deficient. This is mainly because methods used in previous studies, such as randomly amplified polymorphic DNA-PCR, ribotyping, and pulsed-field gel electrophoresis (PFGE), were considered not robust enough for strain delineation and phylogenetic analysis of large numbers of isolates (24,25,26). Kohara et al (24) carried out PFGE on human fecal B. longum isolates and revealed that 12 participants harbored strains of unique PFGE types or subtypes over a 68-week period.…”

Section: Discussionmentioning

confidence: 99%

“…This is mainly because methods used in previous studies, such as randomly amplified polymorphic DNA-PCR, ribotyping, and pulsed-field gel electrophoresis (PFGE), were considered not robust enough for strain delineation and phylogenetic analysis of large numbers of isolates (24,25,26). Kohara et al (24) carried out PFGE on human fecal B. longum isolates and revealed that 12 participants harbored strains of unique PFGE types or subtypes over a 68-week period. Aires et al (25) compared three human B. longum isolates with the sequenced model strain B. longum NCC2705 by PFGE and two-dimensional gel electrophoresis, and 37 proteins were found to differ between these strains.…”

Section: Discussionmentioning

confidence: 99%

The Host Genotype and Environment Affect Strain Types of Bifidobacterium longum subsp. longum Inhabiting the Intestinal Tracts of Twins

Zhang

Hang²,

Tan

et al. 2015

Appl Environ Microbiol

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bTo investigate the influences of host genotype and environment on Bifidobacterium longum subsp. longum inhabiting human intestines at the strain level, six pairs of twins, divided into two groups (children and adults), were recruited. Each group consisted of two monozygotic (MZ) twin pairs and one dizygotic (DZ) twin pair. Child twins had been living together from birth, while adult twins had been living separately for 5 to 10 years. A total of 345 B. longum subsp. longum isolates obtained from 60 fecal samples from these twins were analyzed by multilocus sequence typing (MLST), and 35 sequence types (STs) were finally acquired. Comparison of strains within and between the twin pairs showed that no strains with identical STs were observed between unrelated individuals or within adult DZ twin pairs. Eight STs were found to be monophyletic, existing within MZ twins and child DZ twins. The similarity of strain types within child cotwins was significantly higher than that within adult cotwins, which indicated that environment was one of the important determinants in B. longum subsp. longum strain types inhabiting human intestines. However, although these differences between MZ and DZ twins were observed, it is still difficult to reach an exact conclusion about the impact of host genotype. This is mainly because of the limited number of subjects tested in the present study and the lack of strain types tracing in the same twin pairs from birth until adulthood.T he human intestine is a complex microbial ecosystem. Recent research has shown that gut microbes control or influence many metabolic pathways in the human host (1, 2, 3). Bifidobacteria, which are naturally present in the colonic microbiota, represent up to 25% of the cultivable fecal bacteria in adults and 80% in infants (4). The interest in bifidobacteria has grown significantly, mainly because their presence has been associated with a healthy microbiota, which has led to their widespread use as functional ingredients in food and pharmaceutical products (4,5,6). Considering the rapidly increasing number of commercial and technological strains, it has been reported that probiotic characteristics such as antioxidant potential are strongly strain dependent (7). The FAO/WHO Guidelines for the Evaluation of Probiotics in Food reports the following: "The current state of evidence suggests that probiotic effects are strain specific" (8). Therefore, the health effects of probiotics as well as successful colonization and studies of their mechanism of action should be linked to a specific strain rather than be at species level. However, it is not clear what kinds of Bifidobacterium strains colonize a person, and little is known about the factors that exert an influence on the various strains.The drivers of intestinal bacteria are multiple and can be categorized as environment associated and host associated. Environment-associated factors include diet, geographical location, and the maternal source in early life (9,10,11,12). A recent study implies that the first bacte...

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Intra-specific composition and succession of Bifidobacterium longum in human feces

Cited by 9 publications

References 10 publications

Survival of Bifidobacterium animalis subsp. lactis OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

Survival of Bifidobacterium animalis subsp. lactis OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

Polymorphism and distribution of putative cell-surface adhesin-encoding ORFs among human fecal isolates of Bifidobacterium longum subsp. longum

The Host Genotype and Environment Affect Strain Types of Bifidobacterium longum subsp. longum Inhabiting the Intestinal Tracts of Twins

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