Survival of Bifidobacterium animalis subsp. lactis OPB-1 in the Gastrointestinal Tract after its Administration in a Milk-free Soybean Product and the Effect on Fecal Microbiota in Healthy Adults

Abstract

The survival of Bifidobacterium animalis subsp. lactis OPB-1 in the gastrointestinal tract after its administration in a milk-free yogurt-like soybean product and effects on fecal microbiota in humans were determined. Twelve healthy volunteers were administered 100 g of yogurt-like soybean product for 3 weeks. The plate count method combined with specific PCR and quantitative real-time PCR were used to estimate the numbers of total Bifidobacterium and B. animalis subsp. lactis in fecal samples. B. animalis subsp. lactis was detected during the administration period but not before. The intake number of B. animalis subsp. lactis OPB-1 was 2.4 × 10 10 CFU/day by the plate count method and 4.2 × 10 13 copies/day by quantitative real-time PCR. However, the number of excreted B. animalis subsp. lactis per day was estimated to be more than 10 times the intake number, by both methods. The percentage of Bifidobacterium increased from 16.9 ± 21.8% to 34.1 ± 20.4% by the plate count method and 18.9 ± 13.4% to 27.8 ± 15.8% by the T-RFLP method after 3 weeks administration.

Effect of Bifidobacterium animalis subsp. lactis GCL2505 on the physiological function of intestine in a rat model

“…lactis proliferates in the human intestine following ingestion, although the intestinal portion where B. animalis ssp. lactis grew was not precisely indicated in previous studies (Matsumoto 2000;Ishizuka et al 2012;Watanabe and Isono 2012). Our study indicated that this remarkable proliferation of B. animalis ssp.…”

“…lactis grew was not precisely indicated in previous studies (Matsumoto ; Ishizuka et al. ; Watanabe and Isono ). Our study indicated that this remarkable proliferation of B. animalis ssp.…”