BackgroundIt is increasingly recognized that Lactobacillus plantarum (L. plantarum) has the ability to protect against Enteropathogenic Escherichia coli (EPEC)-induced damage of the epithelial monolayer barrier function by preventing changes in host cell morphology, attaching/effacing (A/E) lesion formation, monolayer resistance, and macromolecular permeability. However, the cellular mechanism involved in this protective effect still remained to be clarified.MethodsThis study was to investigate the effect of L. plantarum on the changes of Caco-2 cells responding to Enteroinvasive Escherichia coli (EIEC), the permeability of cell monolayer and the transmissivity of dextran, and the distribution and expression of the tight junction (TJ) proteins, such as Claudin-1, Occludin, JAM-1 and ZO-1 were examined when infected with EIEC or adhesived of L. plantarum after infection by confocal laser scanning microscopy (CLSM), immunohistochemistry and Western blotting, the cytoskeleton protein F-actin were observed with FITC-phalloidin.ResultsThis study demonstrated that the transepithelial electrical resistance (TER) step down and dextran integrated intensity (DII) step up with time after infected with EIEC, but after treating with L. plantarum, the changes of TER and DII were improved as compared with EIEC group. L. plantarum prevented the damage of expression and rearrangement of Claudin-1, Occludin, JAM-1 and ZO-1 proteins induced by EIEC, and could ameliorate the injury of cytoskeleton protein F-actin infected with EIEC.ConclusionL. plantarum exerted a protective effect against the damage to integrity of Caco-2 monolayer cells and the structure and distribution of TJ proteins by EIEC infection.

The probiotics could improve the gut microflora disturbance, increase occludin expression, maintain the gut epithelial tight junction and decrease the bacterial translocations rate.

This study was conducted to evaluate the diversity of bile salt hydrolase (BSH) activities in eight species of lactobacilli. BSH activities were quantified based on the amount of taurine or glycine liberated from six main human bile sodium salts [glycocholic, glycodeoxycholic, glycochenodeoxycholic, taurocholic (TC), taurochenodeoxycholic, taurodeoxycholic] and a mixture of bile salts that resembled human bile. The eight species differed in their BSH activities. Specifically, Lactobacillus helveticus, Lactobacillus fermentum and Lactobacillus gallinarum had the ability to deconjugate taurine-conjugated bile salts, but not glycine-conjugated bile salts, which suggested that microbial BSHs recognize bile salts on both the cholate steroid nucleus and the amino acid moiety. Of the eight species evaluated, Lactobacillus acidophilus strains exhibited the highest specific BSH activity toward human bile salts, with the exception of TC. In addition, the L. acidophilus specific BSH activity toward glycine-conjugated bile salts was ten times higher than that toward taurine-conjugated bile salts. Moreover, the specific BSH activity of Lactobacillus plantarum did not vary significantly toward different bile salts, and Lactobacillus gasseri Am1 exhibited higher specific BSH activity toward TC than other lactobacilli. A comparison of bsh genes indicated that the LA-bshA, LAbshB, LG-bsh and LP-bsh1 genes that encode the BSH enzymes are highly homologous (higher than 45%), while the LP-bsh2, LP-bsh3 and LP-bsh4 genes, which might not encode BSH enzymes, had lower similarity (lower than 26.3%).

Oral administration of LP was effective in the treatment of colitis, with the direct modification of gut microflora in IL-10(-/-) mice. This probiotic strain could be used as a potential adjuvant in the therapy of inflammatory bowel disease, although further studies are required in human.