Internal damage in potato tubers: a critical review

McGarry, A.; Hole, C. C.; Drew, R. A. L.; Parsons, Nicholas R.

doi:10.1016/0925-5214(96)00006-3

Cited by 61 publications

(25 citation statements)

References 57 publications

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“…However, the extent of the necrosis is in¯uenced by the severity of the impact as it is not seen in every case of internal blackspot (McGarry et al 1996). Under the experimental conditions used in this study, cell death was initiated about 3 h after impact.…”

Section: Discussionmentioning

confidence: 99%

“…Because of the importance of potato as a major crop, the alleviation of potato bruising, or internal black spot, has been a signi®cant target in breeding and genetic engineering (McGarry et al 1996). Damage occurs during harvesting and storage, and is a substantial problem since it is only detected by peeling or cutting the tuber and thus is not easily screened.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the location of polyphenol oxidase in potato ( Solanum tuberosum L.) tuber during cell death in response to impact injury: comparison with wound tissue

Partington

Smith

Bolwell

1999

Planta

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In order to elucidate the nature of the response of potato to impact injury at the biochemical level, changes in the location of the enzyme responsible for the discoloration, polyphenol oxidase, were determined using immunogold location with an antibody specific for potato tuber polyphenol oxidase. Tissue printing revealed that the enzyme was distributed throughout the tuber. Following impact injury, both tissue printing and quantitative electron microscopy indicated that there was no increase in the level of the enzyme although there was subcellular redistribution of polyphenol oxidase. This redistribution was first apparent at 12 h after impact, as determined by the use of confocal immunolocation, and coincided with loss of membrane integrity. These changes were examined in parallel with a number of stress-related parameters in both impact and wound responses. Wounding was accompanied by active gene expression and protein synthesis, leading to metabolic activity and tissue repair. In contrast, the bruising response was characterised by a limited active response and vital-staining methods indicated that after 16 h the tissue undergoes cell death.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in the location of polyphenol oxidase in potato ( Solanum tuberosum L.) tuber during cell death in response to impact injury: comparison with wound tissue

Partington

Smith

Bolwell

1999

Planta

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“…This was probably a result of oxidative reactions involving phenols and the enzyme polyphenol oxidase (PPO) in melanin biosynthesis. It was suggested that in undamaged potato cells PPO and its substrates are kept apart by intracellular compartmentation (McGarry et al, 1996;Cobb, 1998). During impact, damage to intracellular membranes allows PPO and its substrate to come together to form melanin, the pigment responsible for the dark discolouration found in bruised potato tissue (Brooks, 1996).…”

Section: Discussionmentioning

confidence: 99%

An Investigation of Wound Healing in Sugar Beet Roots Using Light and Fluorescence Microscopy

2001

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Following impact, wound healing was investigated in roots of sugar beet using¯uorescence microscopy in conjunction with a conventional lignin test. Samples of sugar beet roots were harvested at dierent stages of development and impacted in the laboratory with a falling bolt delivering 1±4 Joules. A response in the form of deposition of brown material, presumed to be melanin, along the outer and inner walls of cells at the wound surface was observed within 3 d of impact. This material eventually became granular in appearance. Formation of a ligno-suberized boundary layer from cells present at the time of impact ®rst occurred in 16-week-old roots 9 d after impact. Intensity of calco¯uor uorescence supported the ®ndings made using light microscopy. As wound healing progressed with time, the intensity of calco¯uor¯uorescence declined, demonstrating interference by wound healing products with calco¯uor binding. Aggressive harvest and subsequent storage dramatically reduced calco¯uor¯uorescence indicating that this dye may have potential value in the assessment of tissue damage.# 2001 Annals of Botany Company

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“…The level of tyrosine content and activity of PPO are considered to be the main factors involved in blackspot bruise formation (McGarry et al 1996). The importance of PPO in discoloration process was confirmed by results in many studies (Coetzer et al 2001;Arican and Gozukirmizi 2003;Urbany et al 2011Urbany et al , 2012.…”

mentioning

confidence: 53%

Diploid Potato Hybrids as Sources of Resistance to Blackspot Bruising

Hara-Skrzypiec¹,

Jakuczun²

2013

Am. J. Potato Res.

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Resistance to bruising is an important quality trait of potato depending on multiple genetic and environmental factors. Thirty one interspecific diploid hybrids and two tetraploid standards were evaluated for resistance to bruising in 2 years. Diploids originated from crosses between wild and primitive Solanum species and dihaploids of S. tuberosum. Two-year mean values of bruising resistance of tested genotypes indicated significant variability of the trait. Analysis of variance showed significant effects of genotype, year and interaction between them on the trait. Broad-sense heritability of bruising resistance was estimated as moderately high, Hb=0.73. Content of L-tyrosine, the main substrate of discoloration reaction, was not significantly correlated with brusing in tested diploid hybrids. The clones, that were resistant to blackspot bruise, also combined quality traits and resistances to many pathogens. All these traits could be transferred together into tetraploid level in 4x×2x crosses via male 2n gametes produced by diploid hybrids.Resumen La Resistencia al daño mecánico es un carácter importante en la calidad de la papa que depende de múlti-ples factores genéticos y del ambiente. Se evaluaron 31 híbridos diploides interespecíficos y dos estándares tetraploides para resistencia a la magulladura en dos años. Los diploides se originaron de las cruzas entre especies silvestres y primitivas de Solanum y dihaploides de S. tuberosum. Los valores de las medias de dos años de resistencia al daño de los genotipos probados indicaron variabilidad significativa del carácter. El análisis de varianza mostró efectos significativos de genotipo, año e interacción entre ellos en el carácter. Heredabilidad, en un amplio sentido de resistencia al daño mecánico, se estimó como moderadamente alta, Hb=0.73. El contenido de L-tirosina, el principal sustrato de la reacción de oscurecimiento no estuvo correlacionado significativamente con el daño en los híbridos diploides probados. Los clones, que fueron resistentes a la mancha oscura, también combinaron los caracteres de calidad y resistencia a muchos patógenos. Todos estos caracteres pudieron transferirse juntos a nivel tetraploide en cruzas 4x × 2x vía gametos macho 2n producidos en híbridos diploides.

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Internal damage in potato tubers: a critical review

Cited by 61 publications

References 57 publications

Changes in the location of polyphenol oxidase in potato ( Solanum tuberosum L.) tuber during cell death in response to impact injury: comparison with wound tissue

Changes in the location of polyphenol oxidase in potato ( Solanum tuberosum L.) tuber during cell death in response to impact injury: comparison with wound tissue

An Investigation of Wound Healing in Sugar Beet Roots Using Light and Fluorescence Microscopy

Diploid Potato Hybrids as Sources of Resistance to Blackspot Bruising

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