Interactive Effects of Azroubine (E 122) and Vitamin C on Mitotic Cell Division, Nucleic Acids Content and Gene Expression

Mahfoz, Hala M.; El-Atroush, H.; Al-Shammrani, S.

doi:10.21608/ejgc.2010.11065

Cited by 3 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results bear a resemblance to those obtained by Gomes et al (2013), who reported that the mitotic index of A. cepa root tips was successively decreased with the increase in dye (sunset yellow, bordeaux red, and tartrazine yellow) concentrations and duration of treatments. The carmoisineinduced mitodepressive effects in A. cepa were also reported by Mahfoz et al (2010). The mitodepressive effects of carmoisine may be attributed to its inhibitory effect on the onset of mitosis, by lengthening the mitotic cycle and/or delaying spindle formation (Polit et al, 2003).…”

Section: Discussionmentioning

confidence: 73%

“…The mitodepressive effects of carmoisine may be attributed to its inhibitory effect on the onset of mitosis, by lengthening the mitotic cycle and/or delaying spindle formation (Polit et al, 2003). Mahfoz et al (2010) suggested that carmoisine inhibits DNA replication by reducing oxidative phosphorylation, which results in lower ATP levels. The significant decline in mitotic index noted in the present study may be due to the interference by the colorants in the cell cycle by inhibiting DNA synthesis or blocking in the G2 phase of the cell cycle or due to the increase in the incidence of chromosomal aberrations with corresponding increase in the concentration of the colorants.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem

Vazhangat¹,

Thoppil²

2016

Turk J Biol

View full text Add to dashboard Cite

The present study investigates the cytotoxic and genotoxic effects of two frequently employed synthetic food colorants, lemon yellow and orange red, using an Allium cepa assay. Uptake of food colorants by root cells at different concentrations and varied exposures significantly altered the cytogenetic system, membrane integrity, mitochondrial function, root growth, and cell division. The food colorants induced significant DNA damage, micronucleus formation, and other severe chromosomal aberrations at genetic level and inhibited cell division as well as root growth. Severe DNA damage, membrane damage, and metabolic inactivation observed in the result are indicative of apoptotic cell death. These results confirm the possible dose-dependent toxicity of these food colorants in plant systems. The chromosomes of plants and animals are morphologically similar and appear to respond towards mutagens in a similar way like mammals, indicating possible damage to the DNA of humans when these colorants are used indiscriminately.

show abstract

Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem

Vazhangat¹,

Thoppil²

2016

Turk J Biol

View full text Add to dashboard Cite

show abstract

Modulation of mutagen-induced genotoxicity by vitamin C and medicinal plants inAllium cepaL.

et al. 2017

View full text Add to dashboard Cite

Assessment of synthetic food dye erythrosine induced cytotoxicity, genotoxicity, biochemical and molecular alterations in Allium cepa root meristematic cells: insights from in silico study

Singh,

Chadha

2024

Toxicology Research

View full text Add to dashboard Cite

Background Synthetic food dyes are being exponentially used in food products and scarce studies regarding their toxicities and safety raise concern. Erythrosine is one of the synthetic food dyes being used in jams, fig, pineapple marmalades, dairy products, soft drinks, pickles, relishes, smoked fish, cheese, ketchup, maraschino cherries and a variety of other foods. Methodology In this study the cyto-genotoxic effect of erythrosine was evaluated, using root meristematic cells of Allium cepa for the cellular and molecular alternations at concentrations 0.1, 0.25, 0.5 and 1 mg/mL. Results The results revealed a significant decrease of 57.81% in the mitotic index after 96 h at the 0.1 mg/mL concentration. In biochemical analysis, the malondialdehyde content increased significantly (5.47-fold), while proline content, catalase activity and superoxide dismutase activity decreased gradually in a concentration-dependent manner showing a maximum decrease of 78.11%, 64.68% and 61.73% respectively at the highest concentration after 96 h duration. The comet assay revealed increased DNA damage with increasing concentration and attenuated total reflectance- Fourier transform infrared spectroscopy (ATR-FTIR) analysis showed significant alterations in biomolecules as indicated by multivariate analysis, i.e. Principal Component Analysis (PCA). Furthermore, molecular docking demonstrated a strong binding energy (Gbest = −11.46 kcal/mol) and an inhibition constant (Ki) of 3.96 nM between erythrosine and the DNA minor groove. Conclusion The present study’s findings revealed the cytotoxic and genotoxic potential of erythrosine on A. cepa root cells. Further, the study also proposed the usefulness of A. cepa as a model system for studying the toxicity of food additives. Highlights Erythrosine showed prominent cytotoxicity with complete inhibition of mitosis at 0.25 mg/mL conc. High level of genotoxicity revealed by 3.68-fold increment in chromosomal aberrations (CAs) frequency at lowest concentration (0.1 mg/mL). The increased MDA level, reduced CAT, SOD activity and proline content showed significant biochemical changes in antioxidant system. Alternations in biomolecules viz. protein, lipid and nucleic acid region showed by ATR-FITR spectrum and confirmed statistically by multivariate analysis of spectrum. In silico model supports the finding as the lower binding free energy of −11.46 kcal/mol between erythrosine and DNA structure was calculated.

show abstract

Interactive Effects of Azroubine (E 122) and Vitamin C on Mitotic Cell Division, Nucleic Acids Content and Gene Expression

Cited by 3 publications

References 28 publications

Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem

Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem

Modulation of mutagen-induced genotoxicity by vitamin C and medicinal plants inAllium cepaL.

Assessment of synthetic food dye erythrosine induced cytotoxicity, genotoxicity, biochemical and molecular alterations in Allium cepa root meristematic cells: insights from in silico study

Contact Info

Product

Resources

About