Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem

Vazhangat, Prajitha; Thoppil, John Ernest

doi:10.3906/biy-1511-26

Cited by 6 publications

(4 citation statements)

References 33 publications

(48 reference statements)

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“…The maximum inhibition of root lengths was observed at the highest concentrations. These results are in accordance with Vazhangat and Thoppil (2016) who found a significant decrease in A. cepa root length.…”

Section: Determination Of Ec 50 Valuessupporting

confidence: 93%

“…It was observed that root lengths decreased with increasing concentration. In previous studies which evaluated the toxic effects of carmoisine, sunset yellow and other azo dyes on plants, it was determined that these colorants cause DNA damage, apoptotic cell death and mitotic abnormalities by reducing root length and cell division (Gomes et al 2013;Dwivedi and Kumar 2015;Vazhangat and Thoppil 2016). The maximum inhibition of root lengths was observed at the highest concentrations.…”

Section: Determination Of Ec 50 Valuesmentioning

confidence: 99%

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The genotoxic effects of some food colorants onZea maysL. var.saccharataSturt

Dikilitaş

Aksoy

2018

Caryologia

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Carmoisine and sunset yellow are organic azo dyes and widely used in food products, drugs and cosmetics. The present study was conducted to evaluate the toxic effects of these colorants on Zea mays with the root and shoot inhibition test, chlorophyll content, α-amylase activity and RAPD PCR technique. In the root inhibition test, EC 50 values for carmoisine and sunset yellow were determined as 20 g l -1 and 22.5 g l -1 , respectively. Chlorophyll a and chlorophyll b levels were decreased when compared to the control group for all of the carmoisine and sunset yellow concentrations. A slight increase was observed in enzyme activity in groups treated with 10 g l -1 and 20 g l -1 of carmoisine, while a decrease in enzyme activity was observed in the group treated with 40 g l -1 of carmoisine. For sunset yellow, a decrease in enzyme activity was observed in all concentrations. In the RAPD test, 71% polymorphism was obtained for carmoisine treated groups. Polymorphism ratio was determined as 53% for the group that was treated with sunset yellow. We concluded that both carmoisine and sunset yellow change the biochemical parameters and have genotoxic effects on Z. mays.

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Section: Determination Of Ec 50 Valuessupporting

confidence: 93%

Section: Determination Of Ec 50 Valuesmentioning

confidence: 99%

The genotoxic effects of some food colorants onZea maysL. var.saccharataSturt

Dikilitaş

Aksoy

2018

Caryologia

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“…De manera que, la prueba de A. cepa aprovecha la consecuencia de la disminución del índice mitótico, expresada en el menor desarrollo radicular, observando así diferencias significativas entre compuestos a distintas concentraciones, y tiempos de exposición. [12,13,14]. Por lo anterior, el objetivo de este estudio fue resaltar la aplicabilidad de la prueba de índice mitótico en células meristemáticas de A. cepa y comparar los datos del % IM obtenido en condiciones ambientales controladas con los % IM reportados en otros estudios en el ensayo control.…”

Section: Introductionunclassified

Estudio comparativo del índice mitótico en el meristemo de Allium cepa L

Méndez¹,

Durán²,

Méndez³

2022

BISTUA

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El bioensayo de porcentaje de índice mitótico en Allium cepa, es utilizado actualmente en varios campos de la ciencia y la industria para determinar el potencial citotóxico de múltiples compuestos, por tener una gran sensibilidad, por ser de fácil acceso, económico y por su practicidad en el momento de realizarse. En el presente estudio se determinó el porcentaje de índice mitótico bajo unas condiciones específicas de crecimiento del meristemo de la raíz A. cepa (tiempo de crecimiento de 4 días, agua de grifo cambiada dos veces al día, temperatura de 20 a 22 °C), para compararla con estudios previos y observar la variabilidad o similitud de los resultados. Se estableció que el porcentaje mitótico bajo las condiciones experimentales para este ensayo fue de 14,92 DE±1.18, el cual presenta concordancia con estudios reportados, pero también hay variabilidad en los datos que puede ser debido a que el porcentaje de índice mitótico puede estar influenciado por las condiciones de desarrollo del meristemo de A. cepa como el tipo de agua en la cual crece el bulbo, el tiempo de crecimiento de las raíces, el fotoperiodo, temperatura y estado fisiológico del bulbo de cebolla. En conclusión, el porcentaje de índice mitótico obtenido presenta una diferencia mínima con otros estudios, siendo un modelo eficaz para su replicación in vitro de la actividad antimitótica.

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“…Hence instead of synthetic colorants, natural ones can promote a perfect and healthy life. Earlier studies on the induction of giant cells [1] and apoptotic activity and DNA damage as well as metabolic inactivation [2] had proved the cytotoxicity of some common synthetic food dyes. T. paniculata is an important dye yielding plant belonging to Combretaceae.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Antioxidant Potential and Antigenotoxicity Analysis Through Hydrogen Peroxide-Induced Oxidative Stress in Terminalia Paniculata Roth

Aswathi¹,

Thoppil²

2020

Asian J Pharm Clin Res

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Objective: The present study is mainly focused on the antigenotoxicity and antioxidant potential of the fruit extract of an important dye yielding plant Terminalia paniculata Roth. Methods: Genoprotective activity of the fruit extract was studied by Allium cepa root tip assay. Three modes of treatment were used to perform the antigenotoxicity, that is, pre-treatment, post-treatment, and simultaneous treatment. For the study of antioxidant potential, four different assays were performed. Results: Antigenotoxicity studies revealed the protective role of the extract in chromosomal aberrations induced by 2% hydrogen peroxide (H2O2) on A. cepa root tip meristem. The fruit extract showed a significant modulatory effect by means of an inhibition percentage and also it showed a characteristic reversing of chromosomal aberrations induced by H2O2. While in the case of antioxidant activity, the plant extract showed an appreciable antioxidant potential. Four different assays were used to determine the antioxidant potential of T. paniculata. Of these 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging activity revealed almost equal effect to that of the standard. Conclusions: In the present investigation, it has been found that the important dye yielding plant T. paniculata has a significant role in various commercial industries such as food, cosmetics, clothes, and pharmaceutics due to its efficient protective role.

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Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem

Cited by 6 publications

References 33 publications

The genotoxic effects of some food colorants onZea maysL. var.saccharataSturt

The genotoxic effects of some food colorants onZea maysL. var.saccharataSturt

Estudio comparativo del índice mitótico en el meristemo de Allium cepa L

Assessment of Antioxidant Potential and Antigenotoxicity Analysis Through Hydrogen Peroxide-Induced Oxidative Stress in Terminalia Paniculata Roth

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