Interactions between osteopontin and vascular endothelial growth factor: Implications for skeletal disorders

Ramchandani, Divya; Weber, Georg F.

doi:10.1016/j.bone.2015.05.047

Cited by 18 publications

(20 citation statements)

References 100 publications

(127 reference statements)

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“…Consistent with its extensive associations with numerous processes, OPN has been shown to be expressed in multiple cell types including fibroblasts, various bone cells, chondrocytes, immune and inflammatory cells, and vascular cells and it is associated with cell attachment via integrins, chemotaxis, and survival/apoptosis (68). In terms of physiological processes, OPN functions in bone mineralization and remodeling, angiogenesis, and immune and cardiovascular functions (68, 69, 73). OPN has also been linked to many pathological conditions such as skeletal disorders, cancer, rheumatoid arthritis, multiple sclerosis, atherosclerosis and heart disease (68, 73–80).…”

Section: Introductionmentioning

confidence: 99%

“…In terms of physiological processes, OPN functions in bone mineralization and remodeling, angiogenesis, and immune and cardiovascular functions (68, 69, 73). OPN has also been linked to many pathological conditions such as skeletal disorders, cancer, rheumatoid arthritis, multiple sclerosis, atherosclerosis and heart disease (68, 73–80). …”

Section: Introductionmentioning

confidence: 99%

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Matricellular proteins in drug delivery: Therapeutic targets, active agents, and therapeutic localization

Sawyer

Kyriakides

2016

Advanced Drug Delivery Reviews

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Extracellular matrix is composed by a complex array of molecules that together provide structural and functional support to cells. These properties are mainly mediated by the activity of collagenous and elastic fibers, proteoglycans, and proteins such as fibronectin and laminin. ECM composition is tissue-specific and could include matricellular proteins whose primary role is to modulate cell-matrix interactions. In adults, matricellular proteins are primarily expressed during injury, inflammation and disease. Particularly, they are closely associated with the progression and prognosis of cardiovascular and fibrotic diseases, and cancer. This review aims to provide an overview of the potential use of matricellular proteins in drug delivery including the generation of therapeutic agents based on the properties and structures of these proteins as well as their utility as biomarkers for specific diseases.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Matricellular proteins in drug delivery: Therapeutic targets, active agents, and therapeutic localization

Sawyer

Kyriakides

2016

Advanced Drug Delivery Reviews

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“…Previous research revealed that OPN levels were upregulated in the first few weeks after long bone fractures (28). Furthermore, OPN is considered an important factor in bone remodeling.…”

Section: Discussionmentioning

confidence: 99%

Effects of polyvinylpyrrolidone-iodine on tendon-bone healing in a rabbit extra-articular model

Zhang

Zhi

Fang

et al. 2017

Experimental and Therapeutic Medicine

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Polyvinylpyrrolidone-iodine (PVP-I) is a broad-spectrum antimicrobial agent, but its effects on tendon-bone healing are unclear. The purpose of this study was to investigate the effects of PVP-I on bone marrow mesenchymal stem cells (BMSCs) in vitro and on tendon-bone healing in vivo. In this study, following investigation of the concentration-dependent effects of PVP-I on the viability and osteogenic differentiation of BMSCs, the appropriate concentration of PVP-I was selected for animal experiments. New Zealand white rabbits received autologous tendon transplantation with and without PVP-I treatment of the graft tendon. Subsequently, histological examination, biomechanical testing and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analyses were conducted. At 6 weeks post-surgery, connective tissue and osteogenesis was observed at the tendon-bone interface in the PVP-I group. At 12 weeks post-surgery, the interface width in the PVP-I group was much narrower compared with that of the control group. Furthermore, the biomechanical properties of the PVP-I group were significantly stronger than those in the control group (P<0.05). RT-qPCR examination revealed that the mRNA levels of bone morphogenetic protein-2 and osteopontin in the PVP-I group were higher than those in the control group at 6 weeks (P<0.05). In conclusion, these results indicated that PVP-I promoted tendon-bone healing via osteogenesis.

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“…In particular, it is reported that Si is important for optimal synthesis of collagen and for activating the hydroxylation enzymes, improving skin strength and elasticity. As regards platelet concentrates, several studies (Kakudo et al, 2008;Kushida et al, 2013) showed that the use of PRP on human dermal fibroblasts markedly Figure 5 Possible effects of treatment with sodium orthosilicate and CGF on the three different human cell lines used: (a) Si and CGF on NHDF: Si treatment seems to act on the enzyme prolyl hydroxylase, allowing the conversion of L-proline amino acid into Hydroxyproline leading to a more efficient synthesis of collagen (Reffitt et al, 2003;Dong et al, 2017); CGF treatment provides growth factors such as TGF-b that induces Col I synthesis (Ohji et al, 1993) increasing the secretion of the enzyme collagenase; (b) Si and CGF on HUVEC: Si treatment seems to increase some vasoactive molecules involved in the processes of oxidative stress (eNOS and AQP1) and could therefore act as a protective factor against vascular alterations (Buffoli et al, 2013); CGF contains powerful angiogenic factors (VEGF and PDGF), which act synergistically, promoting angiogenesis; (c) Si and CGF on HOBs: Si stimulates the activity of prolyl hydroxylase (Carlisle et al, 1981;Carlisle and Alpenfels, 1984), stimulating Col I synthesis in human osteoblast-like cells; CGF contains osteogenic growth factors (BMPs, VEGFs, PDGFs, and IGFs) that enhances osteoblasts proliferation (Yun et al, 2012) and the expression of bone markers such as Col I and OPN (Ramchandani and Weber, 2015). This is probably due to the Si biological mechanism of action.…”

Section: Discussionmentioning

confidence: 99%

In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines

Bonazza

Borsani

Buffoli

et al. 2017

Cell Biology International

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The aim of this study was to investigate the in vitro effect of Silicon, in the soluble form of sodium orthosilicate, combined and not with the concentrated growth factors (CGF), a platelet-rich preparation, on three different human cell lines of fibroblasts (NHDF), endothelial cells (HUVEC), and osteoblasts (HOBs). Each cell type was treated with sodium orthosilicate at the final concentration of 0.5 mM and 1 mM, CGF, and sodium orthosilicate combined with CGF, for 72 h. At the end of the experimental period, the in vitro effect on cell growth, proliferation, and metabolic activity was evaluated by performing a simple cell count, using an automated cell counter and by evaluating the expression of the intracellular proliferation marker Ki-67, using Fluorescence-activated cell sorting (FACS) analysis. Moreover, the expression of other cell markers and active molecules, such as Collagen type I, Osteopontin, Vascular Endothelial Growth Factor, and endothelial Nitric Oxide Synthase, was evaluated, through immunohistochemistry. Results obtained showed that the combined use of CGF and sodium orthosilicate stimulates cell growth, proliferation, and metabolic activity, suggesting that this treatment could be effective in tissue regeneration.

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Interactions between osteopontin and vascular endothelial growth factor: Implications for skeletal disorders

Cited by 18 publications

References 100 publications

Matricellular proteins in drug delivery: Therapeutic targets, active agents, and therapeutic localization

Matricellular proteins in drug delivery: Therapeutic targets, active agents, and therapeutic localization

Effects of polyvinylpyrrolidone-iodine on tendon-bone healing in a rabbit extra-articular model

In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines

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