In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines

Bonazza, Veronica; Borsani, Elisa; Buffoli, Barbara; Parolini, Silvia; Inchingolo, Francesco; Rezzani, Rita; Rodella, Luigi Fabrizio

doi:10.1002/cbin.10908

Cited by 23 publications

(21 citation statements)

References 48 publications

(107 reference statements)

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“…After dehydration and mounting with DPX, cells were detected using light microscopy. Digitally fixed images were produced and analyzed using a dedicated image software (Image Pro-Premier, Immagini & Computer, Milan, Italy) and the Integrated Optical Density (IOD) was measured and normalized to cell number [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…Undifferentiated HOB (without extracellular calcium deposits) were slightly reddish, whereas mineralized osteoblasts (with extracellular calcium deposits) were bright orange-red. Digitally fixed images of cells were analyzed using an image analyzer (Image Pro-Premier, Immagini & Computer, Milan, Italy) and the Integrated Optical Density (IOD) was measured and normalized to cell number [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…Based on these considerations, the growth factors delivered by CGF and the antioxidant effect of resveratrol could have a synergic role in bone deposition and protection against the side effects of bisphosphonates. So, the aim of the present study was to investigate the in vitro effect of treatment with CGF and/or resveratrol on the proliferation (3 days) and differentiation (21 days) of human osteoblast cells (HOB), treated or not with bisphosphonates monitoring indicative molecular recognised markers, such as osteoprotegerin (OPG) [ 28 ], bone morphogenetic protein-2 (BMP-2) [ 29 ], collagen type I (Col I) [ 30 ], sirtuin 1 (SIRT-1) [ 31 ], and Alizarin Red S staining [ 32 ] for calcium deposits.…”

Section: Introductionmentioning

confidence: 99%

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Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human OsteoblastsIn VitroTreated with Bisphosphonates

Borsani

Bonazza

Buffoli

et al. 2018

BioMed Research International

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Bisphosphonates are primary pharmacological agents against osteoclast-mediated bone loss and widely used in the clinical practice for prevention and treatment of a variety of skeletal conditions, such as low bone density and osteogenesis imperfecta, and pathologies, such as osteoporosis, malignancies metastatic to bone, Paget disease of bone, multiple myeloma, and hypercalcemia of malignancy. However, long-term bisphosphonate treatment is associated with pathologic conditions including osteonecrosis of the jaw, named BRONJ, which impaired bone regeneration process. Clinical management of BRONJ is controversy and one recent approach is the use of platelet concentrates, such as Concentrated Growth Factors, alone or together with biomaterials or antioxidants molecules, such as resveratrol. The aim of the present study was to investigate the in vitro effects of Concentrated Growth Factors and/or resveratrol on the proliferation and differentiation of human osteoblasts, treated or not with bisphosphonates. Human osteoblasts were stimulated for 3 days in complete medium and for 21 days in mineralization medium. At the end of the experimental period, the in vitro effect on osteoblast proliferation and differentiation was evaluated using different techniques such as MTT, ELISA for the quantification/detection of osteoprotegerin and bone morphogenetic protein-2, immunohistochemistry for sirtuin 1 and collagen type I, and the Alizarin Red S staining for the rate of mineralization. Results obtained showed that Concentrated Growth Factors and/or resveratrol significantly increased osteoblast proliferation and differentiation and that the cotreatment with Concentrated Growth Factors and resveratrol had a protective role on osteoblasts treated with bisphosphonates. In conclusion, these data suggest that this approach could be promised in the clinical management of BRONJ.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human OsteoblastsIn VitroTreated with Bisphosphonates

Borsani

Bonazza

Buffoli

et al. 2018

BioMed Research International

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“…By summarising the results of the above‐mentioned clinical case treatment and in vitro coculture of HaCaT cells with CGF membrane, we propose the possible mechanisms for CGF gel or membrane in treating chronic ulcers as follows: Possible mechanisms that promote the generation of granulation tissue: In the process of producing CGF gel, fractional centrifugation can cause platelets to release their growth factors and cytokines, which can facilitate wound healing The stem cells and monocytes present in the CGF gel can move to the bottom of the wound, proliferate, and differentiate further into macrophages to facilitate wound healing …”

Section: Discussionmentioning

confidence: 99%

Use of concentrate growth factors gel or membrane in chronic wound healing: Description of 18 cases

Kao¹

2019

International Wound Journal

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Treating chronic skin wounds in patients with diabetes, bed sores, or stasis dermatitis is typically a time-consuming and costly process, and the outcome is not always promising. Concentrated growth factor (CGF) obtained from the autologous venous blood of patients via fractional centrifugation is employed for producing a CGF gel or membrane that can be applied to expedite self-regeneration of skin wounds. In this case report, we presented the results from 18 patients with chronic skin wounds treated with a CGF gel or membrane produced from autologous venous blood. Noticeable granulation tissue and regenerated epidermal coverage were observed in 16 patients who received CGF treatment over various time courses, thereby demonstrating the significant therapeutic effects of CGF treatment in overall wound healing. The other two patients with stasis ulcers in their calves failed to respond to the treatment because of the comorbidity of iliac vein thrombosis. In addition, by culturing HaCaT keratinocytes using CGF membrane as the foundation, we observed that HaCaT cells attached to the CGF membrane migrated and proliferated to form an epithelium-like structure. Comprehensively, the clinical results infer that CGF gel can expedite the regeneration of the soft tissue at the wound, whereas CGF membrane may facilitate its marginal re-epithelialisation. The combination of the two can promote autologous regeneration of both deep and superficial wounds effectively and safely.

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“…Rodella et al also demonstrated the existence of CD34 positive cells in CGF. CGF stimulates the cell growth, proliferation and metabolic activity of many different cell types in vitro [33], and CGF has also increased graft angiogenesis and wound healing around the sites of implants in vivo [34,35]. Masuki et al found that the platelet-rich fibrin clot exudates resulted in reduced levels of growth factors [15].…”

Section: Discussionmentioning

confidence: 99%

Concentrated Growth Factor from Autologous Platelet Promotes Hair Growth in Androgenetic Alopecia

Tan¹,

Xie²,

Wu³

et al. 2019

JBiSE

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Studies have shown that platelet concentrates can induce the proliferation of the dermal papilla and the vascularization of the perifollicular tissue, as well as accelerate the telogen-to-anagen transition, thereby promoting the regrowth of hair improving the appearance of hair loss. Herein, we report on the application of a new, modified form of platelet concentrates, namely, concentrated growth factors (CGFs), in 15 cases of androgenetic alopecia (AGA). 15 cases of androgenetic alopecia were treated with the use of monthly, subcutaneous injections of autologous CGF in the scalp. A total of 3 injections were administered 4 weeks apart, and the patients were followed up for 6 months. Assessments were performed before the treatments and at 4, 8, 12 and 24 weeks after the first treatment. The treatment outcomes were assessed by taking macroscopic photographs and trichoscopic photomicrographs, as well as by using the Global Aesthetic Improvement Scale (GAIS) and the patient satisfaction survey. In order to determine the safety of the treatment, the injection area was observed for signs of infection or mass evaluation. The photographs showed significant improvement in hair appearance after injections of CGF. The hair photomicrographs showed that CGF promoted the regrowth of hair in balding areas, with an increased hair density and an increased ratio of terminal to vellus hair. The GAIS suggested that CGF treatments were effective in treating AGA, and the majority of patients were satisfied with their improvement. In addition, treatments resulted in a faster rate of hair growth and a decrease in the greasy and unpleasant sensation of the hair of the patients. At the last visit, none of the 15 patients reported experiencing side-effects during the follow-up period. To conclude, the application of CGF can be an effective method in the treatment of androgenetic alopecia.

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In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines

Cited by 23 publications

References 48 publications

Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human OsteoblastsIn VitroTreated with Bisphosphonates

Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human OsteoblastsIn VitroTreated with Bisphosphonates

Use of concentrate growth factors gel or membrane in chronic wound healing: Description of 18 cases

Concentrated Growth Factor from Autologous Platelet Promotes Hair Growth in Androgenetic Alopecia

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