Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human OsteoblastsIn VitroTreated with Bisphosphonates

Abstract

Bisphosphonates are primary pharmacological agents against osteoclast-mediated bone loss and widely used in the clinical practice for prevention and treatment of a variety of skeletal conditions, such as low bone density and osteogenesis imperfecta, and pathologies, such as osteoporosis, malignancies metastatic to bone, Paget disease of bone, multiple myeloma, and hypercalcemia of malignancy. However, long-term bisphosphonate treatment is associated with pathologic conditions including osteonecrosis of the jaw, named BRONJ, which impaired bone regeneration process. Clinical management of BRONJ is controversy and one recent approach is the use of platelet concentrates, such as Concentrated Growth Factors, alone or together with biomaterials or antioxidants molecules, such as resveratrol. The aim of the present study was to investigate the in vitro effects of Concentrated Growth Factors and/or resveratrol on the proliferation and differentiation of human osteoblasts, treated or not with bisphosphonates. Human osteoblasts were stimulated for 3 days in complete medium and for 21 days in mineralization medium. At the end of the experimental period, the in vitro effect on osteoblast proliferation and differentiation was evaluated using different techniques such as MTT, ELISA for the quantification/detection of osteoprotegerin and bone morphogenetic protein-2, immunohistochemistry for sirtuin 1 and collagen type I, and the Alizarin Red S staining for the rate of mineralization. Results obtained showed that Concentrated Growth Factors and/or resveratrol significantly increased osteoblast proliferation and differentiation and that the cotreatment with Concentrated Growth Factors and resveratrol had a protective role on osteoblasts treated with bisphosphonates. In conclusion, these data suggest that this approach could be promised in the clinical management of BRONJ.

In vitro and in vivo effects of concentrated growth factor on cells and tissues

“…Tubes are centrifuged using the built‐in program (30 s acceleration, 2 min at 408 × g , 4 min at 323 × g , 4 min at 408 × g , 3 min at 503 × g , and 36 s deceleration and stop). Then, the intermediate gel layer in the final three‐layered product, which is CGF, should be separated from the third layer by scissor, and could be used as prepared or freeze‐dried (Borsani et al, ).…”

Effect of concentrated growth factors on frequency of alveolar Osteitis following partially-erupted mandibular third molar surgery: a randomized controlled clinical study

“…More randomized clinical studies with larger sample sizes are needed to understand the effects on AO occurrence following lower third molar surgery [ 40 , 43 ]. The preventive qualities of AO act to prohibit bacterial formation and to control bleeding until the socket is healed [ 44 ].…”

Resveratrol Rescue Indoxyl Sulfate-Induced Deterioration of Osteoblastogenesis via the Aryl Hydrocarbon Receptor /MAPK Pathway

“…Primary osteoblast cells were seeded in 96-well plates (1 × 10 3 cells/well) with an osteogenic induction medium for 24 and 48 h and were treated at different concentrations (0, 20, 100, 250, 500, and 1000 μM) of IS with or without 10 μM RSV. The working RSV concentration was set according to an osteoblast pilot study [ 57 ]. The cell viability was measured using a Cell Counting Kit-8 (CCK-8) assay kit (Dojindo Molecular Technologies, Inc.; Rockville, MD, USA), as previously described [ 4 ].…”