Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Martins, F.S.; Celestino, J.J.H.; Saraiva, M.V.A.; Chaves, Roberta Nogueira; Rossetto, R.; Silva, C.M.G.; Lima-Verde, I.B.; Lopes, C.A.P.; Campello, C.C.; Figueiredo, J.R.

doi:10.1590/s0100-879x2010007500066

Cited by 28 publications

(24 citation statements)

References 37 publications

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“…Our findings are similar to those obtained by Silva et al (2006), which did not find any effect on follicular activation of media containing FSH, despite having reported an increase in follicular and oocyte diameter. However, this finding differs from other studies (Martins et al, 2010;Saraiva et al, 2010) which, although they have worked with different culture media, they showed a reduction in the percentage of primordial follicles, concomitant with the increase in the percentage of developing follicles after one day of culture. The same authors showed that, after the 7 th day of culture, this reduction of primordial follicles and the elevation of developing follicles were even more striking and expressive.…”

Section: Ultrastructural Features Of Caprine Preantral Folliclescontrasting

confidence: 99%

“…However, the medium containing T 4 (10)/IGF-I showed an increase in oocyte diameter. It can be suggested that IGF-I has a relevant participation in the increase of follicular diameter, corroborating the results found earlier by Demeestere et al (2005) and Martins et al (2010). This effect seems to be linked to an increase of the mitotic rate of granulosa cells and an increase in oocyte size.…”

Section: Ultrastructural Features Of Caprine Preantral Folliclessupporting

confidence: 89%

“…The same authors showed that, after the 7 th day of culture, this reduction of primordial follicles and the elevation of developing follicles were even more striking and expressive. The data, found by these authors, allowed them to conclude that the growth factor present in the tested media was able to induce follicular activation (Martins et al, 2010). Similar results were found in goats (Silva et al, 2006;Matos et al, 2007).…”

Section: Ultrastructural Features Of Caprine Preantral Folliclessupporting

confidence: 64%

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Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

Costa

Pereira

et al. 2014

RBCV

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AbstratThe aim of this study was to investigate the influence of the interaction between follicle stimulating hormone obtained from Human Pituitary (hFSH; 10 ng/mL), Insulin-like Growth Factor Type 1 (IGF-I; 100 ng/mL) and thyroxine (T 4 ; 10, 20 ng/mL) on survival, activation and growth of preantral follicles (PAF) cultured in vitro. Fragments of ovarian cortex collected from 17 nonpregnant adult goats underwent different treatments that included α-MEM + medium, IGF-I, hFSH, T 4 and were processed for classic histology and transmission electron microscopy. The results showed a reduction in the percentage of normal follicles, after one and seven days of culture in media α-MEM + (cultured control), T 4 (10)/IGF-I, T 4 (20)/IGF-I/hFSH and in media α-MEM + , IGF-I/hFSH, T 4 (20)/IGF-I/hFSH, T 4 (20)/IGF-I/hFSH , when compared to the non-cultured control. However, the medium containing T 4 (20)/hFSH showed a follicular survival rate higher than the other treatments, when cultured for seven days. It was also observed that the follicular diameter did not change when cultured in this medium, when compared to the control (non-cultured). The ultrastructural analysis confirmed the integrity of follicles cultured in a medium containing T 4 (20)/hFSH. In conclusion, the medium containing T 4 (20)/hFSH showed the best results, when evaluating the survival, growth and ultrastructural aspects of caprine preantral follicles cultured in vitro.Keywords: Caprine, hormone, in vitro culture, preantral follicles, ultrastructural analysis. ResumoO objetivo deste estudo foi investigar a influência da interação entre o Hormônio Folículo Estimulante obtido da pituitária Humana (hFSH; 10 ng/mL), Fator de Crescimento Semelhante a Insulina Tipo 1 (IGF-I; 100 ng/mL) e Tiroxina (T 4 ; 10, 20 ng/mL) na sobrevivência, ativação e crescimento de folículos pré-antrais cultivados in vitro. Fragmentos do córtex ovariano coletados a partir de 17 cabras adultas não gestantes foram submetidos a diferentes tratamentos que incluíam meio α-MEM +, IGF-I, hFSH, T 4 e em seguida foram processados para histologia clássica e microscopia eletrônica de transmissão. Os resultados demonstraram uma redução no percentual de folículos normais, após um e sete dias de cultivo nos meios α-MEM + (controle cultivado), T 4 (10)/IGF-I, T 4 (20)/IGF-I/hFSH e nos meios α-MEM + , IGF-I/hFSH, T 4 (20)/IGF-I/hFSH, T 4 (20)/IGF-I/hFSH , quando comparados ao controle não cultivado. Entretanto, o meio contendo T 4 (20)/hFSH apresentou taxa de sobrevivência folicular superior aos demais tratamentos, quando cultivado por sete dias. Observou-se, ainda, que o diâmetro folicular quando cultivado neste meio não se alterou, quando comparado ao controle (não cultivado). As análises ultraestruturais confirmaram a integridade de folículos cultivados em meio contendo T 4 (20)/hFSH. Em conclusão, o meio com T 4 (20)/hFSH apresentou os melhores resultados, quando se avaliou sobrevivência, crescimento e aspectos ultraestruturais de folículos pré-antrais de caprinos cultivados in vitro.Palavras-c...

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Section: Ultrastructural Features Of Caprine Preantral Folliclescontrasting

confidence: 99%

Section: Ultrastructural Features Of Caprine Preantral Folliclessupporting

confidence: 89%

Section: Ultrastructural Features Of Caprine Preantral Folliclessupporting

confidence: 64%

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Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

Costa

Pereira

et al. 2014

RBCV

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“…It is well established that paracrine signals from the oocyte play an essential role in the growth and development of the ovarian follicle. Oocyte-derived growth differentiation factor-9 (GDF-9) has been shown to favor the oocyte growth and follicular development (Hayashi et al 1999;Martins et al 2010). In this study, goat secondary follicles exposed for a period of six-day to GDF-9, FSH or both compounds, had an increase in the follicular diameter when compared to uncultured secondary follicles, but not when compared to follicles that had been cultured in MEM alone.…”

Section: Discussionmentioning

confidence: 81%

“…For treatments, control culture medium was supplemented with 50 ng/mL of FSH (rFSH ® , Nanocore, Brazil), 200 ng/mL of GDF-9 (Sigma, St. Louis, USA) or both. The concentrations of FSH and GDF-9 were those that promoted the highest growth rates in in-vitro goat preantral follicles in previous studies (Matos et al 2007;Martins et al 2010). Fresh media was prepared and incubated for 1h prior to use.…”

Section: Isolation and In Vitro Culture Of Goat Secondary Folliclesmentioning

confidence: 99%

Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles

Leitão

Costa

Brito

et al. 2014

Braz. arch. biol. technol.

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The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for reduced the expression of BMP-2 and -15 in caprine preantral follicles after their culture, but FSH either alone or in association with GDF-9 did not control the expression of

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Long-term in vitro culture of ovarian cortical tissue in goats: effects of FSH and IGF-I on preantral follicular development and FSH and IGF-I receptor mRNA expression

et al. 2012

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Long-term in vitro culture (16 days) of caprine ovarian cortical tissue was performed to test the effect of FSH and IGF-I on the viability and development of preantral follicles and mRNA expression for FSH and IGF-I receptors. Fragments were cultured in α-MEM(+) alone or supplemented with different combinations of FSH and IGF-I (sequential medium). The culture period was divided into two parts. Follicles were isolated and classified as normal or abnormal and primordial, primary or secondary. Viability of isolated follicles was determined by staining with Trypan Blue dye. Expression of FSHR and IGFR-1 mRNA was evaluated by qPCR. At day 8 of culture, more (P < 0.05) follicles in treatments containing IGF-I alone or associated with FSH were normal and viable (overall mean, 81 % and 79 % respectively) than the treatments cultured with FSH or α-MEM(+) alone (68 % and 63 %). At day 16 of culture, treatments with FSH and/or IGF-I had more (P < 0.05) viable follicles (69 %) than α-MEM(+) (38 %). The percentages of follicular development observed in the IGF-I/FSH, FSH+IGF-I/FSH+IGF-I and FSH/IGF-I treatments were similar but higher (P < 0.05) than the other treatments. FSH and IGF-I during the entire culture period maximized (P < 0.05) follicular and oocyte diameters and the percentage of secondary follicles (28 %). FSHR mRNA expression in the non-cultured control was similar to the treatment supplemented with FSH and IGF-I but higher (P < 0.05) than α-MEM(+). IGFR-1 expression did not differ among treatments. Association of FSH and IGF-I in long-term in vitro culture promoted follicular development, maintaining FSHR mRNA expression.

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Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Cited by 28 publications

References 37 publications

Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles

Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles

Long-term in vitro culture of ovarian cortical tissue in goats: effects of FSH and IGF-I on preantral follicular development and FSH and IGF-I receptor mRNA expression

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