Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles

Abstract

The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for reduced the expression of BMP-2 and -15 in caprine preantral follicles after their culture, but FSH either alone or in association with GDF-9 did not control the expression of

Growth and Differentiation Factor-9 Supplementation Affects Viability and Morphology of Preantral Follicles in Equine Ovarian Fragments During Short-term in vitro Culture

“…Although the factors and mechanisms that involve folliculogenesis are not well elucidated, it is possible to affirm that GDF-9 plays an important role in early folliculogenesis, follicular differentiation and steroid hormone synthesis [18,19]. GDF-9 is so essential that in an experiment with GDF-9-depleted rats and sheep, primordial follicles remained at this stage of development, and these animals became sterile [8,20]. Based on these findings and our results, the effect of GDF-9 on in vitro follicular development is confirmed.…”

“…This growth factor assists in the proliferation of granulosa and theca cells, contributes to the growth of primary follicles and promotes the maintenance of follicular viability [5,6]. Furthermore, GDF-9 promoted the survival and continuation of follicular development to the secondary stage in goats [7,8]. GDF-9 is a protein secreted by the oocyte and is considered a potent regulator of ovarian activity, stimulating the proliferation and maintenance of follicular cells.…”

Manipulação De Oócitos Inclusos Em Folículos Ovarianos Pré-Antrais E a Importância Dos Antioxidantes – Revisão De Literatura

“…(penicilina e estreptomicina), selênio, piruvato, glutamina, fatores de crescimento (IGF, EGF, ativina-A, GDF-9 e FGFb), hormônios (insulina, FSH, GH e TSH) e esteroides (estradiol, testosterona ou androstenediona) (RODRIGUES et al, 2010;ANDRADE et al, 2012;LEITÃO et al, 2014).…”

Estratégias para a melhoria da eficiência do cultivo folicular in vitro: Importância da suplementação do meio e estudo das alterações epigenéticas

“…O CIV utiliza meios de base extremamente ricos em nutrientes, eletrólitos, antioxidantes, aminoácidos, substratos energéticos, vitaminas, tampões, hormônios e/ou fatores de crescimento (Picton, Harris, Muruvi, & Chambers, 2008;Figueiredo, Rodrigues, Amorim, & Silva, 2008). Meios de base comerciais como o Meio Essencial Mínimo-alfa modificado (α-MEM), Meio de Cultivo de Tecido 199 (TCM 199) e McCoy's têm sido utilizados no CIV em diferentes espécies (Arunakumari, Shanmugasundaram, & Rao, 2010;Mclaughlin & Telfer, 2010;Leitão et al, 2014).…”