Integration of trinucleotide microsatellites into a linkage map of Citrus

Kijas, James; Thomas, Mark R.; Fowler, Joanna C.; Roose, Mikeal L.

doi:10.1007/s001220050468

Cited by 181 publications

(118 citation statements)

References 28 publications

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“…A 1:1 proportion as expected for the pseudo-testcross configuration was used, since it was hypothesized that the dominant markers were in heterozygosity for one of the parents (Aa) and in homozygosity for the other parent (aa). High levels of deviations were observed in several crossings involving the genus Citrus, independently of the species and the type of marker used (Durham et al, 1992;Jarrell et al, 1992;Cai et al, 1994;Luro et al, 1994;Kijas et al, 1997). There is a high genetic similarity between 'Pêra' sweet orange and 'Cravo' mandarin (Oliveira et al, 2002), even though the levels of distortion observed in Group the segregation were similar to those of intergeneric crossings.…”

Section: Resultsmentioning

confidence: 99%

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Oliveira

Cristofani²,

Machado³

2004

Pesq. agropec. bras.

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-The objective of this work was to construct linkage maps of 'Pêra' sweet orange [Citrus sinensis (L.) Osbeck] and 'Cravo' mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F 1 hybrids, which were obtained from controlled crossings. The linkage map of 'Pêra' sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of 'Cravo' mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM. Only two markers did not link to the groups and 15.7% showed segregation distortion. The construction of linkage maps is relevant to future mapping studies of the inheritance of CVC, citrus canker and leprosis.

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Section: Resultsmentioning

confidence: 99%

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Oliveira

Cristofani²,

Machado³

2004

Pesq. agropec. bras.

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“…After screening of the 25 SSR primers, seven primers from Ahmad et al (2003) and two primers from Kijas et al (1997) were selected for final PCR amplification and data scoring. Using these nine selected primers, in total 49 polymorphic Fig.…”

Section: Ssr Polymorphism and Genetic Relationshipsmentioning

confidence: 99%

“…Among many molecular markers, Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) markers have been used in citrus in a wide range of applications including cultivar identification (Fang and Roose 1997;Novelli et al 2000;Shahsavar et al 2007;Biswas et al 2010;Uzun et al 2010), phylogenetics (Pang et al 2003;Marak and Laskar 2010), zygotic and nucellar seedlings differentiation (Ruiz et al 2000;Oliveira et al 2002;Krueger and Roose 2003) and the construction of linkage maps for marker assisted breeding and map-based cloning of genes (Kijas et al 1997;Sanker and Moore 2001).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Sharafi

Abkenar

Sharafi

et al. 2016

Physiol Mol Biol Plants

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Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime Bwitches' broom disease^.

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“…Thirty-five microsatellite loci were used to analyse the triploid progenies: CAC15, TAA41 (Kijas et al, 1997), CX6F03, CX6F23 (Chen et al, 2007), mest121 , mest56, mest192, mest123 (Aleza et al, 2011), mest104, mest110, mest247, mest488 (François Luro, personal communication; mail to luro@ corse.inra.fr for further information), mCrCIR07F11 (Kamiri et al, 2011), Ci01C07, Ci02B07, Ci08C05, mCrCIR 01E02, mCrCIR01F04a, mCrCIR06A12, mCrCIR06B05, mCrCIR06B07, mCrCIR07E12 and thirteen new designed primers (mCrCIR01C06, mCrCIR0 2A09, mCrCIR02D09, mCrCIR02F12, mCrCIR02G01, mCrC IR02G02, mCrCIR03B07, mCrCIR03C08, mCrCIR03G05, mCrCIR04H06, mCrCIR05A05, mCrCIR07D05, mCrCIR07 D06; Table 1). The polymerase chain reactions (PCRs) were performed with wellRED oligonucleotides (Sigma-Aldrich, St Louis, MO, USA) with the following protocol: Mastercycler epgradient S (Eppendorf Scientific Inc., Westbury, NY, USA); reaction volume 15 ml: 0.8 U Taq polymerase (Fermentas, Burlington, VT, USA), reaction buffer 750 mM Tris-HCl (pH 9), 50 mM KCl, 200 mM (NH 4 ) 2 SO 4 , 0.001% bovine serum albumin, 0.1 mM of each dNTP, 5 mM MgCl 2 , 3 mM of each primer, 30 ng DNA; PCR programme: 94 1C for 5 min; 40 cycles of 30 s at 94 1C, 1 min at 50-55 1C and 30 s at 72 1C; final elongation 10 min at 72 1C).…”

Section: Plant Materialsmentioning

confidence: 99%

Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv ‘Fortune’

et al. 2011

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The genetic structure of 2n gametes and, particularly, the parental heterozygosity restitution at each locus depends on the meiotic process by which they originated, with firstdivision restitution and second-division restitution (SDR) being the two major mechanisms. The origin of 2n gametes in citrus is still controversial, although sexual polyploidisation is widely used for triploid seedless cultivar development. In this study, we report the analysis of 2n gametes of mandarin cv 'Fortune' by genotyping 171 triploid hybrids with 35 simple sequence repeat markers. The microsatellite DNA allele counting-peak ratios method for alleledosage evaluation proved highly efficient in segregating triploid progenies and allowed half-tetrad analysis (HTA) by inferring the 2n gamete allelic configuration. All 2n gametes arose from the female genitor. The observed maternal heterozygosity restitution varied between 10 and 82%, depending on the locus, thus SDR appears to be the mechanism underlying 2n gamete production in mandarin cv 'Fortune'. A new method to locate the centromere, based on the best fit between observed heterozygosity restitution within a linkage group and theoretical functions under either partial or no chiasmata interference hypotheses was successfully applied to linkage group II. The maximum value of heterozygosity restitution and the pattern of restitution along this linkage group would suggest there is partial chiasma interference. The implications of such a restitution mechanism for citrus breeding are discussed.

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Integration of trinucleotide microsatellites into a linkage map of Citrus

Cited by 181 publications

References 28 publications

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv ‘Fortune’

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