Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Abstract

Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime Bwitches' broom disease^.

Molecular genetic diversity assessment of Citrus species grown in Iran revealed by SSR, ISSR and CAPS molecular markers

“…'Siavaraz' is a local cultivar of sweet orange with a long history of cultivation in northern part of Iran, Mazandaran and Guilan provinces (Sharafi et al, 2016). It is a highly seedy and juicy cultivar with unknown origin (Sharafi et al, 2016). In the present study with a slight divergence this cultivar was found very closely related to Frost Valencia' and 'Washington Navel.…”

“…The similarity value between them was 0.77. 'Siavaraz' is a local cultivar of sweet orange with a long history of cultivation in northern part of Iran, Mazandaran and Guilan provinces (Sharafi et al, 2016). It is a highly seedy and juicy cultivar with unknown origin (Sharafi et al, 2016).…”

“…We suggest that it could be a hybrid of 'Bakraii'. 'Persian' lime is a unique and new cultivar to citrus industry of Iran and is not being cultivated widespread (Sharafi et al, 2016). The flowers of 'Persian' lime are devoid of viable pollen also contain exceedingly few functional ovules (Mantel, 1967).…”

“…Probably for these reasons, no accession was closely clustered with 'Persian' lime in this study, but it was located in acid lime cluster. Acid lime has a long history of cultivation in the south regions of Iran such as Fars, Hormozgan, Kerman, Bushehr and Sistan-Baluchestan provinces (Sharafi et al, 2016). Too many hectares of acid lime orchards have been established in these provinces.…”

Investigation and DNA Analysis of Local <i>Citrus</i> Genetic Resources Grown on the Chuuk Islands of Micronesia

“…To elucidate the precise traits of each accession, investigations of morphological traits and DNA analysis have been very effective (Ueda and Higuchi, 2012). New findings regarding several local citrus genetic resources were also reported using both morphological and molecular markers (Nasir et al, 2017;Sharafi et al, 2016;Tshering Penjor et al, 2014a, b).…”