Insecticidal and Cytotoxic Activities of Extracts of <I>Cacalia tangutica</I> and Its Two Active Ingredients Against <I>Musca domestica</I> and <I>Aedes albopictus</I>

Abstract

The insecticidal activities of extracts from different parts of Cacalia tangutica (Franch.) H.-M. and two active ingredients, friedelin and stigmasterol, against Musca domestica L., Aedes albopictus (Skuse), and Spodoptera litura (F.) cells (SL cells) are reported. Extracts from leaves, flowers, and roots are highly active against adult M. domestica and the fourth-instar larvae of Ae. albopictus. The foliar extract was the most active with LC50 values of 1.74 mg/g and 196.94 microg/ml against adult M. domestica and fourth-instar larvae of Ae. albopictus, respectively. Extracts from stems were the least active. Friedelin and rotenone were highly active and equitoxic to these two insect species. The LC50 value of friedelin in adult M. domestica 48 h after treatment was 129.27 microg/g. The activity of stigmasterol on these two insect species was not obvious. However, the cytotoxicity of stigmasterol to S. litura cells was significantly greater than that of either friedelin or rotenone. At 25 microg/ml, the mortality of cells 24 h after treatment with stigmasterol was 23.0%. In contrast, friedelin and rotenone at the same concentration produced 16 and 18% mortality, respectively. Our results indicate differences between cytotoxicity in vitro and insect toxicity in vivo.

Assessment of camel thorn (Alhagi maurorum) as new sources of bioactive compounds using GC-MS technique

“…This is an antidiabetic compound (Sujatha et al, 2010), which is able to inhibit the growth of cancer cells such as lung (Mendilaharsu et al, 1998), gastric (De Stefani et al, 2000), and ovary (McCann et al, 2003) cancers. It also has anti-tumor activity (Ekade and Manik, 2014), antioxidant (Zawistowaski, 2010), anti-osteoarthitic (Gabay et al, 2010), cyto-toxicity activity (Huang et al, 2009) and anti-HIV reverse transcriptase (De Oliveira et al, 2014). Ergost-5en-3-ol (campesterol) is a phytosterol which has been proven to be able to inhibit various cancer cells, such as lung (Mendilaharsu et al, 1998), gastric (De Stefani et al, 2000), and ovary (McCann et al, 2003) cancers.…”

Differential anti-insect activity of natural products isolated from Dodonaea viscosa Jacq. (Sapindaceae)

“…For example, the feeding deterrence against Spodoptera litura Fabricius (Lepidoptera: Noctuidae) and Spilosoma obliqua Walker (Lepidoptera: Arctiidae) (Tandon et al 1998), and cytotoxic activity against S. litura (Huang et al 2009). Stigmasterol, compound 2, was also reported as a component of an active extract against the bug Collaria oleosa Distant (Hemiptera: Miridae) (Saez et al 1998) as well as of an active extract against M. persicae (Santana et al 2012).…”

Insecticidal Activity of Torricellia tiliifolia Extracts Against Musca domestica and Aedes albopictus

“…Acetone alone was used as a control. When the acetone was completely evaporated, leaving most of the dissolved compounds as a coating on the sugar, 10 house ßies were introduced into a vial and then closed with a screened screw cap (Xu et al 2003, Huang et al 2009). Mortality was recorded at 24 and 48 h. Each treatment was replicated three times.…”