Inheritance and biochemical analysis of four electrophoretic variants of β-conglycinin from soybean

Davies, Corinne S.; Coates, J. B.; Nielsen, Niels Chr.

doi:10.1007/bf00252079

Cited by 36 publications

(18 citation statements)

References 14 publications

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“…Three of these regions hybridize specifically to 2.5 kb mRNAs that encode c~/e' subunits, while the others hybridize either to 1.7 kb mRNAs that encode/3 subunits or to both kinds of mRNAs equally well. These data are in contrast to genetic segregation information obtained using multiple alleles that encode aberrant ~ or ~' genes [7]. The segregation data show that a single genetic locus is responsible for the complete complement of ~' subunits found in the seed of the varieties tested, and that a second independently segregating gene locus is responsible for all of the ~ subunits.…”

Section: The ~-Conglycinin Subunit Encoded By Pccg215 3 Is Defectivementioning

confidence: 43%

“…The segregation data show that a single genetic locus is responsible for the complete complement of ~' subunits found in the seed of the varieties tested, and that a second independently segregating gene locus is responsible for all of the ~ subunits. Together the data of Harada et al [ 12] and Davies et al [7] imply that the complexity of the gene family is likely due to the presence of non-functional genes. Moreover, the clustering of the genes in the genome and the similarities between the DNA sequences for the three types of fl-conglycinin genes increase the probability of unequal crossover events.…”

Section: The ~-Conglycinin Subunit Encoded By Pccg215 3 Is Defectivementioning

confidence: 85%

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Synthesis and assembly of soybean ?-conglycinin in vitro

Lelièvre

Dickinson

et al. 1992

Plant Mol Biol

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The construction of SP6-derived expression plasmids that encode normal and modified beta-conglycinin subunits is described. With the exception of an additional methionine at their NH2-terminal ends and the lack of glycans, the normal subunits synthesized at the direction of these plasmids corresponded to mature alpha and beta subunits isolated from soybean seeds. The subunits assembled into trimers in vitro that were equivalent in size to those formed in vivo. This result shows that the glycans are not required either for protein folding or oligomer assembly. Subunits produced from other plasmids, which had modifications in a highly conserved hydrophobic region in the COOH-terminal end of the subunits, either did not assemble or assembled at an extremely low rate compared to unmodified subunits. Structural changes at the more hydrophilic NH2-terminal end had mixed effects. Several subunits modified in this region assembled into trimers at rates that were either equal or greater than those for normal alpha subunits. Others assembled less completely than the normal subunits. Our results indicate that the in vitro synthesis and assembly assay will be useful in evaluating structure-function relationships in modified beta-conglycinin subunits. The results also show that structural changes at the NH2-terminal end of the subunits are tolerated to a greater extent than modifications in the hydrophobic conserved region in the COOH-terminal half of the subunits, and this information will be useful in efforts to improve soybean quality.

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Section: The ~-Conglycinin Subunit Encoded By Pccg215 3 Is Defectivementioning

confidence: 43%

Section: The ~-Conglycinin Subunit Encoded By Pccg215 3 Is Defectivementioning

confidence: 85%

Synthesis and assembly of soybean ?-conglycinin in vitro

Lelièvre

Dickinson

et al. 1992

Plant Mol Biol

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“…A number of soybean seed-protein mutants with changes in either their charge or size have been reported (Orf and Hymowitz 1979;Hildebrand and Hymowitz 1980;Davies et al 1985). However, the precise molecular events responsible for these changes remain unclear.…”

Section: Discussionmentioning

confidence: 97%

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene

Wei

Satoh

et al. 2003

Theor Appl Genet

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The rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1H/alpha-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6.50/alpha-1. Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6.50/alpha-1 of high homology with the deleted pI6.71/alpha-2 polypeptide which was encoded by glu4 (GluA-1). The nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene. These results suggested the possibility of altering glutelin quality by using single gene mutation.

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“…However, genetic analysis of the b-subunit gene(s) has proven very difficult because quantitative variation of this subunit is associated with changes in environmental conditions (Bray and Beachy 1985;Gayler and Sykes 1985;Holowach et al 1986;). Furthermore, a-and b-subunits have been found to be tightly linked (Kitamura et al 1984;Davies et al 1985;Tsukada et al 1986;Phan 1996). Although stable a¢-null and a-null mutants have been identified (Kitamura and Kaizuma 1981;Takahashi et al 1994), a b-less variant has not been found.…”

Section: Introductionmentioning

confidence: 98%

Genetic Diversity of Soybean (Glycine Max (L.) Merrill)7S Globulin Protein Subunits

Liu

Ohta

Dong

et al. 2006

Genet Resour Crop Evol

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Out of 851 soybean accessions from Vietnam, China and Japan analyzed for 7S b-subunit variants, a new b-reduced subunit line with normal growth was collected from the Mekong Delta, Vietnam. Protein of the 'b-reduced' line is composed of b-reduced and extremely low-b types. (a-null + b-reduced) type and b-null (or extremely low-b) type were screened in the progeny seeds of the Japanese mutant '(a + b) null' line. Therefore, recombination between a-and b-subunits was identified. By comparing the nucleotide sequence of the partial b-subunit gene of Enrei (standard), Mo-shi-dou Gong 503 (a + b low), b-5 33 seed (b-reduced), b-5 7 seed (extremely low-b), and (a + b)-null 16 ((a + b) null) seed, we found that the base 'T' at 166 bp, in Enrei changed to 'G' in Mo-shi-dou Gong 503, b-5 33 and b-5 7 . Using the (a + b)-null 16 individual as template, a distinct 305 bp b-subunit gene fragment was identified, instead of a 285 bp fragment.Abbreviations: bp -base pair; kDa -kilo dalton

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Inheritance and biochemical analysis of four electrophoretic variants of β-conglycinin from soybean

Cited by 36 publications

References 14 publications

Synthesis and assembly of soybean ?-conglycinin in vitro

Synthesis and assembly of soybean ?-conglycinin in vitro

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene

Genetic Diversity of Soybean (Glycine Max (L.) Merrill)7S Globulin Protein Subunits

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