Genetic Diversity of Soybean (Glycine Max (L.) Merrill)7S Globulin Protein Subunits

Abstract

Out of 851 soybean accessions from Vietnam, China and Japan analyzed for 7S b-subunit variants, a new b-reduced subunit line with normal growth was collected from the Mekong Delta, Vietnam. Protein of the 'b-reduced' line is composed of b-reduced and extremely low-b types. (a-null + b-reduced) type and b-null (or extremely low-b) type were screened in the progeny seeds of the Japanese mutant '(a + b) null' line. Therefore, recombination between a-and b-subunits was identified. By comparing the nucleotide sequence of the partial b-subunit gene of Enrei (standard), Mo-shi-dou Gong 503 (a + b low), b-5 33 seed (b-reduced), b-5 7 seed (extremely low-b), and (a + b)-null 16 ((a + b) null) seed, we found that the base 'T' at 166 bp, in Enrei changed to 'G' in Mo-shi-dou Gong 503, b-5 33 and b-5 7 . Using the (a + b)-null 16 individual as template, a distinct 305 bp b-subunit gene fragment was identified, instead of a 285 bp fragment.Abbreviations: bp -base pair; kDa -kilo dalton

Characterization of Soybean Storage and Allergen Proteins Affected by Environmental and Genetic Factors

“…Variation in storage proteins among genotypes has been reported previously in peas as well as soybeans. , Zilic et al analyzed β-conglycinin and glycinin subunits in seven soybean genotypes by SDS-PAGE and reported that the amounts of the various subunits varied among genotypes. Liu et alalso used SDS-PAGE to analyze β-conglycinin in 851 soybean accessions from Vietnam, China, and Japan and reported genetic variability among them.…”

Alteration of Seed Storage Protein Composition in Soybean [Glycine max (L.) Merrill] Mutant Lines Induced by γ-Irradiation Mutagenesis

“…In addition, the β subunit of β-conglycinin is not a desirable soybean protein subunit because it contains no methionine residues . Therefore, by producing a recombinant β-conglycinin subunit with suppressed expression of the β subunit of soybean β-conglycinin, it should be possible to improve the quality of soybean . In previous studies, several mutants of 7S globulin subunits were obtained. ,,, Manjaya et al reported that the mutants M-17 and M-231 can be identified by the lack of α′ and α subunits of β-conglycinin and by increased levels of β subunits of β-conglycinin.…”

Breeding Canola (Brassica napus L.) for Protein in Feed and Food

“…Diversity in SSP composition has been reported in various crop species such as wheat [ 245 ] and soybean [ 246 ], implying similar diversity may exist in canola. Wild soybean accessions with unique SSP profiles have been successfully incorporated into breeding programs to generate segregating populations with variable seed protein profiles [ 247 ] indicating that the manipulation of storage protein composition can be achieved by conventional breeding.…”