Alteration of Seed Storage Protein Composition in Soybean [Glycine max (L.) Merrill] Mutant Lines Induced by γ-Irradiation Mutagenesis

Abstract

This study investigated the alteration of seed storage proteins in soybean mutants induced by γ-irradiation. Five soybean cultivars and four landraces were irradiated with 250 Gy of γ rays to induce variability. The seed storage protein profiles of 414 genetic fixed mutants (M(12)-M(20)) having excellent agricultural traits were analyzed by SDS-PAGE. Among the 414 mutants, 58 were identifed as lacking lipoxygenase, 89 lacking the α' subunit, 113 lacking the α subunit, and 40 with an altered β subunit. One hundred and forty-nine mutants lacked the A(3) subunit of glycinin. Fifty-four mutants showed higher trypsin inhibitor (TIA) activity, whereas 139 showed lower TIA activity compared to their original cultivars. The selected mutants with low amounts of antinutritional factors such as trypsin inhibitor, lipoxygenase, and α subunit will constitute genetic resources for improving soybean protein quality.

Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich δ-zein in transgenic soybeans

“…Several naturally occurring, as well as induced, mutations that affect the accumulation of soybean seed storage proteins have been previously described (Kitamura and Kaizuma, 1981; Odanaka and Kaizuma, 1989; Takahashi et al, 1994; Yagasaki et al, 1996; Hayashi et al, 1998). By integrating these mutations by crossbreeding, a soybean line that lacks all the glycinin and β-conglycinin subunits has been developed (Takahashi et al, 2003).…”

Construction of Soybean Mutant Diversity Pool (MDP) Lines and an Analysis of Their Genetic Relationships and Associations Using TRAP Markers

“…Various plant organisms, such as seeds, pollen, whole plants, and embryoid bodies, can be irradiated [7]. Because gamma rays can also cause various types of DNA damage, including single-or double-strand breaks and substitutions [8,9], agronomic traits, such as flowering, maturation date, seed coat color, chloroplast number, and biomass yield, are frequently altered in soybean [10,11]. At present, 3200 mutant varieties of more than 210 plant species have been produced for commercial use.…”

From proteomics to ionomics: Soybean genetic improvement for better food safety

“…Later on, mutation induction using gamma-ray irradiation was applied to develop strains with individual or all β-conglycinin subunits being absent (e.g. TAKAHASHI et al, 1994;MANJAYA et al, 2007;LEE et al, 2011). Thus, hypoallergenic soybeans were developed such as the Japanese cultivar "Yumeminori" lacking α-and α'-subunits of β-conglycinin with an additional absence of the Gly m Bd 28 K allergen (TAKAHASHI at al.…”

“…In contrast to the Kunitz trypsin inhibitor, significant variation including null variants of the Bowman-Birk inhibitors have been discovered in wild species of the genus Glycine only which cannot be introgressed easily into Glycine max (DOMAGALSKI et al, 1992;SONG et al, 2016). Mutation induction might therefore be a feasible way towards a further reduction of the overall trypsin inhibitor activity (MANJAYA et al, 2007;LEE et al, 2011). Moreover, the Bowman-Birk inhibitor appears to be coded by at least three different gene loci with different alleles/isoforms present (TAN-WILSON et al, 1987).…”