Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

Kim, Won‐Seok; Jez, Joseph M.; Krishnan, Hari B.

doi:10.3389/fpls.2014.00633

Cited by 36 publications

(30 citation statements)

References 79 publications

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“…Further results were suggestive of a higher methionine concentration in the transgenic line in response to high sulfur and high nitrogen, although this could not be analyzed statistically due to a lack of replication. Recently, Kim et al (2014) reported that adequate sulfate nutrition is required to maximize the accumulation of maize δ-zein in transgenic soybean lines where expression of the endogenous β-conglycinin was suppressed by RNA interference.…”

Section: Discussionmentioning

confidence: 99%

Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition

et al. 2015

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It has been hypothesized that the relatively low concentration of sulfur amino acids in legume seeds might be an ecological adaptation to nutrient poor, marginal soils. SARC1 and SMARC1N-PN1 are genetically related lines of common bean (dry bean, Phaseolus vulgaris) differing in seed storage protein composition. In SMARC1N-PN1, the lack of phaseolin and major lectins is compensated by increased levels of sulfur-rich proteins, resulting in an enhanced concentration of cysteine and methionine, mostly at the expense of the abundant non-protein amino acid, S-methylcysteine. To identify potential effects associated with an increased concentration of sulfur amino acids in the protein pool, the response of the two genotypes to low and high sulfur nutrition was evaluated under controlled conditions. Seed yield was increased by the high sulfate treatment in SMARC1N-PN1. The seed concentrations of sulfur, sulfate, and S-methylcysteine were altered by the sulfur treatment in both genotypes. The concentration of total cysteine and extractible globulins was increased specifically in SMARC1N-PN1. Proteomic analysis identified arcelin-like protein 4, lipoxygenase-3, albumin-2, and alpha amylase inhibitor beta chain as having increased levels under high sulfur conditions. Lipoxygenase-3 accumulation was sensitive to sulfur nutrition only in SMARC1N-PN1. Under field conditions, both SARC1 and SMARC1N-PN1 exhibited a slight increase in yield in response to sulfur treatment, typical for common bean.

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Section: Discussionmentioning

confidence: 99%

Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition

et al. 2015

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“…The application of glutathione to immature soybean cotyledons reduced the accumulation of the β-subunit of β-conglycinin and increased the accumulation of glycinin (Awazuhara et al, 2002). Further, Kim et al (2014) demonstrated that ample supply of reduced sulphur during seed filling promotes soybean protein quality by reducing the accumulation of sulphur-poor 7S β-conglycinins. Likewise, providing an exogenous source of methionine to soybean plants results in 23 and 31% increase in methionine and cysteine content, respectively (Yi et al, 2010).…”

Section: Resultsmentioning

confidence: 96%

Effect of nitrogen and sulphur nutrition on storage protein quality in soybean [Glycine max (L.) Merrill]

Sharma

2018

JANS

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Abstract:The relative availabilities of nitrogen and sulphur can modulate seed storage protein composition in grain legumes and cereals. Soybean contains two major seed storage proteins, glycinins and β-conglycinins that account for approximately 70% of total protein and their composition is affected by nitrogen and sulphur supplies. The present study demonstrated the effect of sulphur (Gypsum @ 20 kg S ha ) alone or in combination on accumulation patterns of various protein fractions and protein quality in soybean seeds under agro-climatic conditions of Punjab. Application of nitrogen or sulphur alone decreased the relative proportion of β-subunit of purified β-conglycinin fraction than control. The acidic polypeptides of glycinin fraction of globulin and 11S/7S ratio were increased in all the treatments in comparison to control, and the maximum increase was reported in the combined application of gypsum with the recommended dose of urea. The lower proportion of total 7S, increased acidic polypeptides and improved 11S:7S ratio by combined treatment of nitrogen and sulphur suggests that gypsum @ 20 kg S ha -1 can be beneficial when applied along the recommended nitrogen dose to improve soybean protein quality.

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“…During the maturation phase, the production of one storage component likely results from repartitioning of carbon amongst storage reserves. Attempts at rebalancing protein and oil have given mixed results, with strategies to enhance protein such as heterologous protein expression [50] and suppression of specific seed storage proteins [51] providing small gains in the overall protein content due to concurrent changes within the proteome whereas the expression of the Arabidopsis thaliana orphan gene QUA-QUINE STARCH (QQS) in soybean seeds resulted in 4-10% increase in protein predominantly at the expense of starch [52,53]. Conversely, natural variants of RAFFINOSE SYNTHASE responsible for RFO accumulation have decreased RFO content and increased sucrose without further gains in oil or protein accumulation [5].…”

Section: Discussionmentioning

confidence: 99%

On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants

et al. 2019

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Protein and oil levels measured at maturity are inversely correlated across soybean lines; however, carbon is in limited supply during maturation resulting in tradeoffs for the production of other reserves including oligosaccharides. During the late stages of seed development, the allocation of carbon for storage reserves changes. Lipid and protein levels decline while concentrations of indigestible raffinose family oligosaccharides (RFOs) increase, leading to a decreased crop value. Since the maternal source of carbon is diminished during seed maturation stages of development, carbon supplied to RFO synthesis likely comes from an internal, turned-over source and may contribute to the reduction in protein and lipid content in mature seeds. In this study, fast neutron (FN) mutagenized soybean populations with deletions in central carbon metabolic genes were examined for trends in oil, protein, sugar, and RFO accumulation leading to an altered final composition. Two lines with concurrent increases in oil and protein, by combined 10%, were identified. A delayed switch in carbon allocation towards RFO biosynthesis resulted in extended lipid accumulation and without compromising protein. Strategies for future soybean improvement using FN resources are described. of 153-12% of biomass is present as sugars and oligosaccharides [4]. Sugars, such as sucrose, represent a metabolizable energy source in animal feed, but raffinose and stachyose that are members of the raffinose family oligosaccharides (RFOs) are indigestible and undesirable for livestock production [5,6]. RFOs have hypothesized roles in seed desiccation tolerance [7-9], stability of liposomes during dehydration, and seed germination [10][11][12], although efforts in breeding and genetic engineering have demonstrated that reduced levels do not impact seed viability [13][14][15]. The RFO and sucrose levels are the result of central carbon metabolism and could adversely impact the levels of oil and protein, which are known to be inversely correlated in mature seeds [16][17][18][19][20][21].Protein and lipid accumulation over the course of seed development is dependent on the supply of amino acids and sugars from the maternal sources (i.e., organic carbon assimilated in the leaf) as well as the metabolism within the developing seed (i.e., cotyledons) [22][23][24][25]. The levels of intermediates of central carbon (C) and nitrogen (N) metabolism that are precursors for storage reserve production, vary depending on the stage of reproductive development and may indicate temporal changes in metabolism [26][27][28]. During maturation, 10-15% of lipids are degraded [28][29][30], coinciding with RFO accumulation, and occurring at the time when there are little to no exogenous resources supplied by the maternal plant. The expression of genes involved in gluconeogenesis and glyoxylate cycle suggest carbon remobilization from lipid may occur. Along with existing sucrose present late in development, carbon from lipid could contribute to central metabolism and enable RFO pro...

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Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich Î´-zein in transgenic soybeans

Cited by 36 publications

References 79 publications

Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition

Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition

Effect of nitrogen and sulphur nutrition on storage protein quality in soybean [Glycine max (L.) Merrill]

On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants

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