Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene

Qu, Le Qing; Wei, Xiaoli; Satoh, Hiroyuki; Kumamaru, Toshihiro; Ogawa, Masahiro; Takaiwa, Fumio

doi:10.1007/s00122-003-1228-x

Cited by 8 publications

(3 citation statements)

References 27 publications

(31 reference statements)

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“…An extensive analysis of the complete rice genome sequence indicates that there are at least 11 glutelin genes ( Katsube-Tanaka et al , 2004 ). However, only GluA-1, GluB-4, and GluB-5 have been conclusively associated with their corresponding genes ( Kusaba et al , 2003 ; Qu et al , 2003 ; Morita et al , 2007 ).…”

Section: Discussionmentioning

confidence: 97%

Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm

Kawakatsu

Yamamoto

Hirose

et al. 2008

Journal of Experimental Botany

153

131

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A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin gene cluster. The limited GluD-1 size polymorphism among the rice varieties is due to amino acid substitutions rather than to post-transcriptional modification. GluD-1 is maximally expressed in the starchy endosperm starting at 5 d after flowering (DAF) and increasing through 30 DAF, a major difference from the other glutelins which are primarily expressed in the subaleurone from 10–16 DAF. Only about 0.2 kb of the GluD-1 promoter was sufficient to confer inner starchy endosperm-specific expression. The 0.2 kb truncated GluD-1 promoter contains a bifactorial endosperm box consisting of a truncated GCN4 motif (TGA(G/C)TCA) and AAAG Prolamin box (P box), and ACGT and AACA motifs as cis-regulatory elements. Gel retardation assays and trans-activation experiments indicated that the truncated GCN4 and P box are specifically recognized by RISBZ1 b-ZIP and RPBF Dof activators in vitro, respectively, and are synergistically transactivated, indicating that combinatorial interactions of these motifs are involved in essential endosperm-specific regulation. Furthermore, deviation from the cognate GCN4 motif alters tissue-specific expression in the inner starchy endosperm to include other endosperm tissues.

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Section: Discussionmentioning

confidence: 97%

Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm

Kawakatsu

Yamamoto

Hirose

et al. 2008

Journal of Experimental Botany

153

131

View full text Add to dashboard Cite

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“…AGPS2 could be coordinated with starch accumulation in the developing rice seed [29]. Moreover, two glu4a genes (JreChr03G12610 and JreChr03G12612) involved in seed glutelin quality (eating quality) [30], and one th1 gene (JreChr03G12627) related to seed weight per panicle in rice [31], were found within the candidate selected region (Chr3: 42.5-42.7 Mb). In addition to genes related to seed traits, we found two genes coding nitrilase with Pfam "PF00795" (carbon-nitrogen hydrolase) within a selected region (Chr13: 20.9-21.3 Mb) identified by two selective approaches (Additional file 7: Table S6).…”

Section: Signatures Of Selection In Walnut Genomesmentioning

confidence: 99%

A genome variation map provides insights into the genetics of walnut adaptation and agronomic traits

Zhang

et al. 2021

Genome Biol

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Background Common walnut (Juglans regia L.) is one of the top four most consumed nuts in the world due to its health benefits and pleasant taste. Despite its economic importance, the evolutionary history and genetic control of its adaptation and agronomic traits remain largely unexplored. Results We report a comprehensive walnut genomic variation map based on whole-genome resequencing of 815 walnut accessions. Evolutionary analyses suggest that Chinese J. regia diverged from J. sigillata with extensive hybridizations after the split of the two species. In contrast to annual crops, the genetic diversity and heterozygous deleterious mutations of Chinese common walnut trees have continued to increase during the improvement process. Selective sweep analyses identify 902 genes uniquely selected in the improved common walnut compared to its progenitor population. Five major-effect loci are identified to be involved in walnut adaptations to temperature, precipitation, and altitude. Genome-wide association studies reveal 27 genomic loci responsible for 18 important agronomic traits, among which JrFAD2 and JrANR are the potentially major-effect causative genes controlling linoleic acid content and color of the endopleura of the nut, respectively. Conclusions The largest genomic resource for walnuts to date has been generated and explored in this study, unveiling their evolutionary history and cracking the genetic code for agronomic traits and environmental adaptation of this economically crucial crop tree.

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“…Qu et al. () reported that the mutant lacked the polypeptide pI6.71/α‐2 encoded by GLU4 gene, while forming a new polypeptide (pI6.50/α‐1), which was encoded by the GLU4a gene. Using IEF and two‐dimensional electrophoresis analysis, Wen and Luthe () and Qu et al.…”

Section: Discussionmentioning

confidence: 99%

Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam

et al. 2014

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The diversity of glutelin acidic polypeptides in rice cultivars collected from Northern Vietnam was characterized via sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) electrophoresis. Glutelin acidic subunits were separated into four bands by molecular mass, as a-1 (39 kDa), a-2 (38 kDa), a-3 (37.5 or 37 kDa) and a-4 (34 or 33 kDa). One hundred and eighty-five Vietnamese rice cultivars were divided into three types, based on differences in staining intensity and the molecular size of the a-3 and a-4 polypeptides derived from SDS-PAGE analysis. Wide variation was also observed in the isoelectric point (pI) staining intensity, in addition to the absence/ presence of pI bands detected via IEF analysis. A total of 16 pI bands, ranging from pI 6.30 to pI 7.52, were identified in the various local rice cultivars. The maximum and minimum of IEF bands detected were 14 and 10, respectively. The genetic variability index (H′) ranged from 0.280 to 0.820, which confirms that local rice cultivars from Northern Vietnam have diverse glutelin seed storage units.

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Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene

Cited by 8 publications

References 27 publications

Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm

Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm

A genome variation map provides insights into the genetics of walnut adaptation and agronomic traits

Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam

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