Influence of silver nanoparticles conditions synthesis on their electrochemical properties

Перевезенцева, Дарья Олеговна; Gorchakov, Eduard; Petrushin, Maksim; Hismutdinov, Ilya; Bimatov, Vladimir

doi:10.1063/1.4964527

Cited by 6 publications

(3 citation statements)

References 16 publications

(12 reference statements)

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“…Figure 6 shows CLS voltammograms of the AgCl reduction on the GCCE when the antigen was present on the electrochemical immunosensor surface (Figure 6, coloured). Free silver ions were prepared by dissolving metallic silver in 1 mol·L −1 HNO 3 , and then AgCl (formed by precipitation of Ag + with Cl − ) was reduced at the potential of +0.1 V on the bare GCCE, thus generating the signal [22]. In the case of the comparative experiments without the immobilized antigen on the surface of the electrochemical immunosensor and with another type of bioconjugates ( Ab 1 @AgNP ), no electrochemical signal indicating the AgCl reduction on the GCCE was detected (Figure 6, black line).…”

Section: Resultsmentioning

confidence: 99%

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

Khristunova

Короткова

Kratochvíl

et al. 2019

Sensors

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A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL−1 to 1600 IU·mL−1, with a detection limit of 50 IU·mL−1. The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.

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Section: Resultsmentioning

confidence: 99%

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

Khristunova

Короткова

Kratochvíl

et al. 2019

Sensors

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“…The main characteristics of the synthesized nanoparticles were determined by electron microscopy, Polydispercity and their corresponding size in the sample was confirmed by absorption spectra [8][9][10]. Pharmaceutical hydrophilic ointment composition was prepared as follows.…”

Section: Preparation Nanoparticlesmentioning

confidence: 99%

“…Various methods are used for silver nanoparticles synthesis, but the most commonly used method is based on the reduction of silver ions in solutions using various reducing agents. Such method is widespread due to its simplicity and availability [7][8][9][10]. This method was used in our study as well.…”

Section: Introductionmentioning

confidence: 99%

Local treatment of burn wounds in animals using a new nanocomponent ointment

et al. 2019

Self Cite

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We have developed an ointment preparation consisting of silver and zinc oxide nanoparticles, which form the basis of the nanocomposition. This drug can reduce the healing time of the wound surface due to the inclusion of nanodispersed particles in the composition. Application of nanoparticles allows to reduce inflammatory process, to carry out disinfecting treatment of a wound surface from pathogenic microorganisms, and accordingly to create optimum conditions for reparative process. Nanocomposite preparation has many advantages over existing analogues without irritating and allergic effects. Due to the high adsorption capacity, it allows to accelerate the healing process of the damaged surface of the skin. Initial studies were conducted on laboratory rats at the age of 6 months with a close mass that was up to 250 g in accordance with international requirements for the use of laboratory animals. As a result of the experiment, a new ointment preparation based on silver and zinc oxide nanoparticles, which are active components of the nanocomposition, was obtained. The use of these components allowed to accelerate the process of reparative restoration of the skin with full recovery of the studied animals. As shown by the experience, the recovery rate on average accelerated by seven days, compared with similar drugs available on the pharmaceutical market.

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Veterinary and Sanitary Assessment of Sheep Slaughter Products for Dermatitis

Aygubov

Gorchakov

Dyachenko

et al. 2021

IOP Conf. Ser.: Mater. Sci. Eng.

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The work is devoted to assessing the quality of livestock slaughter products, taking into account skin diseases of various etiologies. This criterion sometimes makes a significant contribution to the quality of livestock products, so it must be taken into account during veterinary and sanitary examination in laboratories. To assess the state of health and predict changes in the quality of products of the slaughter of sheep, great importance belongs to the study of patterns in the clinical, anamnestic, morphological, metric, and anatomical indicators of the body of the sheep. The use of these patterns and their dynamic changes characteristics helps to find out certain patterns that made it possible to develop several rules for the modern veterinary and sanitary assessment of sheep slaughter products. These rules allow not only to indicate the premisses for the occurrence of dermatitis of various etiologies but also to improve existing developments, as well as improve the quality of preventive remedies to prevent and eliminate these types of diseases. It should also be noted that for the start of preventive steps, the strict observance of elementary rules is important when grazing the number of sheep. This includes their movement in compliance with certain periods, the seasonality of the use of forage land, and the mandatory inspection of the livestock of animals and their parking places. In the case of the detection of infected animals with signs of damage to the skin, the animals are immediately isolated with subsequent diagnosis and treatment.

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Influence of silver nanoparticles conditions synthesis on their electrochemical properties

Cited by 6 publications

References 16 publications

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

Local treatment of burn wounds in animals using a new nanocomponent ointment

Veterinary and Sanitary Assessment of Sheep Slaughter Products for Dermatitis

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