Influence of nanoscale surface topography on protein adsorption and cellular response

Lord, Megan S.; Foss, Morten; Besenbacher, Flemming

doi:10.1016/j.nantod.2010.01.001

Cited by 527 publications

(414 citation statements)

References 105 publications

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“…An improvement and acceleration of the healing after surgical intervention would cause a tremendous enhancement of life quality. Therefore, finding optimized functional materials that are able to attract or repel specific molecules is recently in a strong focus of life sciences [4][5][6]. Thus, the question arises how one can improve and modify the interface between a specific material and its biological surrounding by subsequent physical treatments, e.g.…”

Section: Introductionmentioning

confidence: 99%

Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

et al. 2012

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We synthesized nano-scaled periodic ripple patterns on silicon and titanium dioxide (TiO2) surfaces by xenon ion irradiation, and performed adsorption experiments with human plasma fibrinogen (HPF) on such surfaces as a function of the ripple wavelength. Atomic force microscopy showed the adsorption of HPF in mostly globular conformation on crystalline and amorphous flat Si surfaces as well as on nano-structured Si with long ripple wavelengths. For short ripple wavelengths the proteins seem to adsorb in a stretched formation and align across or along the ripples. In contrast to that, the proteins adsorb in a globular assembly on flat and long-wavelength rippled TiO2, but no adsorbed proteins could be observed on TiO2 with short ripple wavelengths due to a decrease of the adsorption energy caused by surface curvature. Consequently, the adsorption behavior of HPF can be tuned on biomedically interesting materials by introducing a nano-sized morphology while not modifying the stoichiometry/chemistry.

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Section: Introductionmentioning

confidence: 99%

Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

et al. 2012

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“…The confirmation, orientation, and quantity of proteins adsorbed are influenced by the surface features, roughness, and oxidation state of the polymers. 35,36 The alignment of PEDOT domains in the matrix of PSS and generation of local defect patterns result in the formation of regions exhibiting variability in the surface potential and conductivity which modulates the overall distribution of proteins adsorbed on the CP substrates. The modulation of cell adhesion results in the variation of traction forces which the cells perceive from the substrate and it leads to the changes in the actin based cytoskeletal assembly.…”

Section: Discussionmentioning

confidence: 99%

“…33,34 The surface charge on the substrate can alter the recruitment of proteins, which in turn influences the formation of focal adhesion complexes and lead to changes in the downstream signaling events. 35,36 We show that a subtle and controlled method of modifying the polymer surface is achieved by stretching. A careful study of cell differentiation on surfaces which have different degrees of strain provides a clear demonstration of the substrate effect.…”

mentioning

confidence: 95%

Neuronal Differentiation of Embryonic Stem Cell Derived Neuronal Progenitors Can Be Regulated by Stretchable Conducting Polymers

Srivastava

Venugopalan²,

Divya³

et al. 2013

Tissue Engineering Part A

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Electrically conducting polymers are prospective candidates as active substrates for the development of neuroprosthetic devices. The utility of these substrates for promoting differentiation of embryonic stem cells paves viable routes for regenerative medicine. Here, we have tuned the electrical and mechanical cues provided to the embryonic stem cells during differentiation by precisely straining the conducting polymer (CP) coated, elastomeric-substrate. Upon straining the substrates, the neural differentiation pattern occurs in form of aggregates, accompanied by a gradient where substrate interface reveals a higher degree of differentiation. The CP domains align under linear stress along with the formation of local defect patterns leading to disruption of actin cytoskeleton of cells, and can provide a mechano-transductive basis for the observed changes in the differentiation. Our results demonstrate that along with biochemical and mechanical cues, conductivity of the polymer plays a major role in cellular differentiation thereby providing another control feature to modulate the differentiation and proliferation of stem cells.

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“…Ti thin films exhibited the highest surface roughness after the 20 min in contact with the serum. It is reported that nanoscale surface could affect protein adsorption and conformation [9], while surface roughness between 2 and 21 nm would not alter protein adsorption amount [10]. Although the surface roughness of the three materials was different, they were all below 6 nm and could be neglected.…”

Section: Contact Angle Measurementsmentioning

confidence: 99%

IN-VITRO IMMUNOCOMPATIBILITY & CHARACTERIZATION AT NANOSCALE OF THIN FILMS AND VASCULAR MEDICAL DEVICES.

Janjic¹,

Kavatzikidou²,

Karagkiozaki³

et al. 2017

IJAR

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Texture.Immunocompatibility comprises a complex response which depends both on the physico-chemical characteristics of the biomaterial and on the hereditary and acquired ability of the recipient to react. Objective: The objective of the present work was the comparative study of the immunocompatibility of different materials and their morphological characterization. Methods: The three types of thin films used in this work were the titanium nitride [TiN],Titanium [Ti] and the amorphous hydrogenated diamond-like carbon [a-C:H], which are deposited onto a silicon substrate by Magnetron Sputtering and Plasma-Enhanced Chemical Vapor Deposition (PECVD), respectively. Medical devices used in this study, were the silicon coated Latex irrigation catheter and the endoprosthesis such as vascular grafts [Dacron, PTFE] and stent grafts [Nitinol stent graft, Cobalt Chromium stent graft]. Bare silicon substrate and serum were used as a "negative" control and the Latex [elastomer-elastic hydrocarbon polymer] as a "positive" control. Complement C5 convertase [C5c] activation was assessed using the sandwich enzyme immunoassay-sandwich ELISA for the specific time periods [0min, 15 min, 30 min and 60 min] at wavelength of 450nm. The morphological characterization of the materials was conducted by Scanning Electron Microscopy (SEM). The study of biomaterials topography was conducted by Atomic Force Microscopy (AFM) and their surface wettability properties by Contact Angle measurements. Results: Our results show that both groups of materials (the nanomaterials and the medical devices) are not likely to cause any immunological adverse reaction and as a result might be characterized and selected as excellent candidates for medical applications. Conclusions: The effectiveness of the study is attributed to the measurement of a single factor in serum in order to acquire important information about the materials' properties e.g immunological response. The possibility to modify the above tested parameters during

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Influence of nanoscale surface topography on protein adsorption and cellular response

Cited by 527 publications

References 105 publications

Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

Neuronal Differentiation of Embryonic Stem Cell Derived Neuronal Progenitors Can Be Regulated by Stretchable Conducting Polymers

IN-VITRO IMMUNOCOMPATIBILITY & CHARACTERIZATION AT NANOSCALE OF THIN FILMS AND VASCULAR MEDICAL DEVICES.

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