Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

Sommerfeld, Jana; Richter, Jessica; Niepelt, Raphael; Kosan, Stefanie; Keller, Thomas F.; Ronning, Carsten

doi:10.1007/s13758-012-0055-5

Cited by 25 publications

(29 citation statements)

References 30 publications

(36 reference statements)

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“…Consistent with our observations, ceramic NPs have been reported to stimulate cell growth to a greater extent than MPs. 30 Protein adsorption is also enhanced on nanostructured silicon, 31 and nano-features increase the formation and adhesive strength of fibroblast cells. 32 Silica-based scaffolds 33 and coated plates 34 have hydrophilic properties.…”

Section: Discussionmentioning

confidence: 99%

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Kim¹,

Joe²,

Kim³

et al. 2015

IJN

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Background Silicon dioxide composites have been found to enhance the mechanical properties of scaffolds and to support growth of human adipose tissue-derived stem cells (hADSCs) both in vitro and in vivo. Silica (silicon dioxide alone) exists as differently sized particles when suspended in culture medium, but it is not clear whether particle size influences the beneficial effect of silicon dioxide on hADSCs. In this study, we examined the effect of different sized particles on growth and mitogen-activated protein kinase signaling in hADSCs. Methods Silica gel was prepared by a chemical reaction using hydrochloric acid and sodium silicate, washed, sterilized, and suspended in serum-free culture medium for 48 hours, and then sequentially filtered through a 0.22 μm filter (filtrate containing nanoparticles smaller than 220 nm; silica NPs). hADSCs were incubated with silica NPs or 3 μm silica microparticles (MPs), examined by transmission electron microscopy, and assayed for cell proliferation, apoptosis, and mitogen-activated protein kinase signaling. Results Eighty-nine percent of the silica NPs were around 50–120 nm in size. When hADSCs were treated with the study particles, silica NPs were observed in endocytosed vacuoles in the cytosol of hADSCs, but silica MPs showed no cell entry. Silica NPs increased the proliferation of hADSCs, but silica MPs had no significant effect in this regard. Instead, silica MPs induced slight apoptosis. Silica NPs increased phosphorylation of extracellular signal-related kinase (ERK)1/2, while silica MPs increased phosphorylation of p38. Silica NPs had no effect on phosphorylation of Janus kinase or p38. Pretreatment with PD98059, a MEK inhibitor, prevented the ERK1/2 phosphorylation and proliferation induced by silica NPs. Conclusion Scaffolds containing silicon dioxide for tissue engineering may enhance cell growth through ERK1/2 activation only when NPs around 50–120 nm in size are included, and single component silica-derived NPs could be useful for bioscaffolds in stem cell therapy.

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Section: Discussionmentioning

confidence: 99%

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Kim¹,

Joe²,

Kim³

et al. 2015

IJN

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“…In particular, the adsorption of human plasma fibrinogen (HPF) was studied as a function of the ripple wavelength [288]. It was observed by AFM that the adsorption of HPF takes place in mostly globular conformation on crystalline and amorphous flat Si surfaces as well as on nano-structured Si with long ripple wavelengths.…”

Section: Applicationsmentioning

confidence: 99%

Self-organized nanopatterning of silicon surfaces by ion beam sputtering

Muñoz-García

Vázquez

Castro

et al. 2014

Materials Science and Engineering: R: Reports

159

201

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“…For instance, the deposition of Ag leads to the formation of aligned Ag nanoparticles influencing the plasmon resonance . Ripple structures could also be used to array functionalized molecules for biomedical on‐chip applications …”

Section: Introductionmentioning

confidence: 99%

Quantitative Imaging of the Magnetic Configuration of Modulated Nanostructures by Electron Holography

Körner

Röder

Lenz

et al. 2014

Small

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By means of off-axis electron holography the local distribution of the magnetic induction within and around a poly-crystalline Permalloy (Ni81Fe19) thin film is studied. In addition the stray field above the sample is measured by magnetic force microscopy on a larger area. The film is deposited on a periodically nanostructured (rippled) Si substrate, which was formed by Xe(+) ion beam erosion. This introduces the periodical ripple shape to the Permalloy film. The created ripple morphology is expected to modify the magnetization distribution within the Permalloy and to induce dipolar stray fields. These stray fields play an important role in spinwave dynamics of periodic nanostructures like magnonic crystals. Micromagnetic simulations estimate those stray fields in the order of only 10 mT. Consequently, their experimental determination at nanometer spatial resolution is highly demanding and requires advanced acquisition and reconstruction techniques such as electron holography. The reconstructed magnetic phase images show the magnetized thin film, in which the magnetization direction follows mainly the given morphology. Furthermore, a closer look to the Permalloy/carbon interface reveals stray fields at the detection limit of the method in the order of 10 mT, which is in qualitative agreement with the micromagnetic simulations.

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Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

Cited by 25 publications

References 30 publications

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Self-organized nanopatterning of silicon surfaces by ion beam sputtering

Quantitative Imaging of the Magnetic Configuration of Modulated Nanostructures by Electron Holography

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