Influence of cadmium loading on glutathione system of antioxidant protection of the bullocks’bodies

Gutyj, B. V.; Gufriy, D. F.; Binkevych, V; Vasiv, R. O.; Demus, N. V.; Leskiv, K. Y.; Binkevych, O; Pavliv, Oleksandra

doi:10.32718/nvlvet9207

Cited by 13 publications

(12 citation statements)

References 10 publications

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“…It is known that under conditions of oxidative stress and excessive generation of AEO, processes of uncontrolled protein modification is developing, that cause protein fragmentation, their denaturation, and also the formation of primary amino acid radicals, further engaging in secondary interaction with adjacent amino acid residues, and in general, it creates a rather complicated picture of the damaging action of AEO on protein macromolecules. All this leads to the loss of their biological activity by proteins and disruption of metabolic processes, in particular regenerative processes (Armstrong, 2002;Martyshuk et al, 2016;Lavryshyn et al, 2016;Gutyj et al, 2018;Lavryshyn & Gutyj, 2019).…”

Section: Resultsmentioning

confidence: 99%

The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C

Romanovych

Kurtyak

Mudrak

2019

Ukr. Jour. of Vet. and Agr. Sci.

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Chickens in the control group received a standard ration, the first experimental – ration with the addition of tocopherol acetate, the second experimental – ascorbic acid, and the third – a complex of these vitamins. On the basis of the conducted researches the influence of vitamin supplements on the indexes of oxidation intensity of lipid and protein structures was analyzed. The intensity of the processes of oxidative modification of proteins largely depends on the level of vitamins E and C in the ration of broiler chickens. Supplementation with vitamin C and with vitamin E resulted in an inhibitory influence on the content of OMP products, the level of which is largely regulated by the enzyme and non-enzymatic links of the antioxidant protection system. Searches have shown the stimulating influence of the addition of vitamins on the activity of antioxidant enzymes. In particular, SOD activity increased by 19 % on the 34th day, by 32 % on the 41st day, whereas glutathione peroxidase activity was 5.7 and 20.8 %, respectively, compared to the control chickens group. An increase in the activity of antioxidant enzymes occurred against the background of a decrease in the intensity of oxidation processes. At the same time, there was also an increase in the content of recovered glutathione in the blood of chickens of the second and third experimental groups at 34th and 41st days of age. Thereby, searches have shown that supplementing the ration with vitamins E and C with a positive influence on the organism's adaptive capacity and antioxidant potential, which is extremely important to ensure the metabolic homeostasis of chickens during critical periods of their cultivation.

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Section: Resultsmentioning

confidence: 99%

The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C

Romanovych

Kurtyak

Mudrak

2019

Ukr. Jour. of Vet. and Agr. Sci.

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“…В результаті появи у гідрофобному шарі мембран гідрофільних зон за рахунок утворення гідропероксидів жирних кислот у клітини можуть проникати вода, іони Натрію, Кальцію, що призводить до набрякання клітин, органел і їх руйнування. Активація пероксидного окиснення характерна для багатьох захворювань, воно активується також у тканинах, які піддалися спочатку ішемії, а потім реоксигенації, що відбувається, наприклад, при спазмі коронарних артерій і подальшому їх розширенні, й тому вивчення впливу лікарських препаратів і біологічно активних добавок, які мають антиоксидантну дію на організм, для корекції ряду станів, що викликані або супроводжуються окисним стресом, є актуальним (Florence, 1995;Men'shchikova et al, 2006;Grivennikova & Vinogradov, 2013;Lototsʹka, 2013;Gutyj et al, 2018;Kharechkina & Nikiforova, 2018;Martyshuk & Gutyj, 2019).…”

Section: вступunclassified

Correction of the state of antioxidant protection system of the fowl’s organism under the circumstances of being poisoned by Derosal

Kochevenko¹,

Zhukova²,

Bobrytska³

et al. 2020

SMLNUVMB

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In fact, Free radical oxidation is one of the required factors of homeostasis as far as the active forms of oxygen, which have unpaired electron, perform useful functions, they are necessary intermediaries of numerous processes of normal cells’ functioning such as biosynthesis of prostaglandins and leukotriene as well as other biologically active substances. Also, they take part in pathological processes such as: inflammation, fever and others. Free radical oxidation is the process of direct oxygen transferring to the substrate forming peroxide, ketones, aldehydes, etc. Some amino acids, proteins, water and carbohydrates prone to the processes of peroxidation, but crucial place is granted to lipids at the expanse of fatty acids which are included to their composition. The article highlights the information concerning the analysis of the state of antioxidant system of hens in the process of feeding them with subtoxic doses of fungicide of Derosal (BAYER, Germany) (0.1 LD50 for a fowl, 900 mg/kg of the body mass) which active ingredient is benzimidazole carbamate (carbendazim, BMC). It presents the changes of these indicators in the process of using pharmacological and natural antioxidants. The analysis has figured out that giving Derosal to the hens without corrections (II group) has been followed with the activation of the process of lipids’ peroxidation (POL) in the blood plasma, that is followed with the increase of concentration of primary and secondary lipoperoxidation products – diene conjugates (DC) and TBA-active products on the average up to 21.0–25.0 %, catalase activity – up to 38.5 % and decrease of superoxide dismutase activity (SOD) up to 21.8 % and АОА – up to 17.8 %, that shows the adverse effects of pesticides on the system of antioxidant protection of the organism. In the fowls’ groups III, IV and V the adding of antioxidant E-selenium, ascorbic acid and thermally untreated buckwheat seeds as the source of quercetins and anthocyanins has led to the induction of antioxidant resources that is shown by the decrease in comparison with the control of content of diene conjugates, malonic dialdehyde and catalase (in average up to 13.6–33.6–18.0 % accordingly) and compensatory strengthening of superoxide dismutase activity and general activity of antioxidant (АОА) on the average up to 44.9–72.1–76.5 % and 15.3–13.4–21.7 % accordingly. In comparison with group II the activity of catalase has been lower as well as the activity of superoxide dismutase has been higher in all the groups almost in 1.5–2 times (Р ≤ 0.001).

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“…Проблема забруднення навколишнього середовища Кадмієм та Свинцем, що є одним із наслідків інтенсифікації промислового й аграрного виробництва, на даний час набула особливої актуальності (Ali et al, 1986;Zhylishchych, 2011;Hutyi, 2015;Gutyj et al, 2018;. Зростання вмісту цих металів в ґрунтах України та інших країн упродовж останніх десятиріч супроводжується нагромадженням Cd 2+ і Pb 2+ у сільськогосподарській продукції та кормах, таким чином виникає збільшення загрози здоров'ю людини і тварин Kuras & Ersteniuk, 2019).…”

Section: вступunclassified

Protein synthesizing function and the functional state of liver of rats for the continuous cadmium and lead load

Slobodian

Gutyj

2019

SMLNUVMB

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The aim of the study was to investigate the effect of Cadmium and Lead on the protein synthesis and function of the rat liver. The experiments were conducted in male rats of the Wistar line, weighing 200–220 g, of which 4 groups of animals were formed: 1) a control group – injected drinking water through a metal probe in a volume equivalent to the volume of an aqueous solution of Cd2+ salts and Pb2+; 2) experimental group 1 – animals were administered a 0.029% aqueous solution of cadmium chloride at a dose of 4.0 mg/kg; 3) experimental group 2 – animals were administered 16.6% aqueous lead acetate solution at a dose of 200 mg/kg; 4) experimental group 3 – animals were administered 16.6% aqueous lead acetate solution at a dose of 100 mg/kg and 0.029% aqueous cadmium chloride solution at a dose of 2.0 mg/kg. Throughout the experiment, rats were kept in a balanced diet containing all the necessary components, and animals were given drinking water, without restriction, from 0.2 liter glass bowls. The functional state of the liver of rats under the conditions of use of salts of heavy metals was investigated by the activity of aminotransferases. In long-term lead-cadmium loading in rats of the experimental groups, the functional state of the liver is characterized, which is characterized by an increase in the permeability of biological membranes of the cell membranes, which causes hyperfermentemia in blood serum, in particular aminotransferase (AST, ALT). The high activity of ALT and AST in the serum of rats under the influence of Cadmium and Lead indicates the destructive processes in the liver that cause an increase in the output of aminotransaminases from cell organelles in the blood of experimental animals. Thus, the results obtained indicate an increase in the destructive processes in the body of rats under lead-cadmium loading. Important diagnostic value for intoxication of different etiology is the determination of protein synthesis of liver function. An important indicator of liver protein synthesis is the level of total protein and its fractions in the serum. This indicator reflects the changes that occur in the body in different pathological conditions. When loaded with heavy metals in the body of rats inhibits the protein synthesis function of the liver, which is manifested by a decrease in total blood protein and albumin levels.

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Influence of cadmium loading on glutathione system of antioxidant protection of the bullocks’bodies

Cited by 13 publications

References 10 publications

The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C

The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C

Correction of the state of antioxidant protection system of the fowl’s organism under the circumstances of being poisoned by Derosal

Protein synthesizing function and the functional state of liver of rats for the continuous cadmium and lead load

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