Infecção hospitalar em Unidade de Terapia Intensiva Neonatal: há influência do local de nascimento?

Pinheiro, Monica de Souza Bomfim; Nicoletti, Christiane; Boszczowsk, Icaro; Puccini, Dilma M. T.; Ramos, Sonia Regina T. S.

doi:10.1590/s0103-05822009000100002

Cited by 18 publications

(17 citation statements)

References 29 publications

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“…The overall NI incidence rates reported in Brazilian NICUs of 30.6% 7 and 22% 8 are much higher than those observed in most studies in the United States (11.4%) 9 or Europe (2.5%) 10 .…”

Section: Resultscontrasting

confidence: 57%

Outbreak of neonatal infection by an endemic clone of Serratia marcescens

Lima

Carvalho

Carneiro

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: The outbreak occurred between February and June 2006 and included identification of the cases, analysis of medical records, cultures from environmental sources, resistance analyses and genotyping profile of Serratia marcescens. Methods: The cultures were composed of 13 blood isolates, 17 rectal and hand swabs and air sampling. Results: The data obtained by pulsed-field gel electrophoresis exhibited three strains that contaminated 24 patients. Systemic infection was the most common in neonates with lower weight, long periods of hospitalization, premature delivery and the use of mechanical ventilation. Conclusions: This investigation revealed the multifactorial nature of the outbreak. An endemic clone of S. marcescens was detected.

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“…The overall NI incidence rates reported in Brazilian NICUs of 30.6% 7 and 22% 8 are much higher than those observed in most studies in the United States (11.4%) 9 or Europe (2.5%) 10 .…”

Section: Resultscontrasting

confidence: 57%

Outbreak of neonatal infection by an endemic clone of Serratia marcescens

Lima

Carvalho

Carneiro

et al. 2011

Rev. Soc. Bras. Med. Trop.

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“…(2)(3)(4)(5)(6) However, the proportion of positive blood cultures among the patients with sepsis (17.1%) was lower than the figures reported in the literature (18% to 65%). (6,7,20,21) The blood cultures from the three patients who died revealed Gram-negative bacteria, raising the possibility that these agents are associated with increased mortality. This finding agrees with CohenWolkowiez et al and Gordon et al, who found that increased mortality ranging from 19% to 24% was related to Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…and Enterobacter spp., were isolated most frequently. (3,6,12,(27)(28)(29) An American study assessing only late-premature children has demonstrated that S. aureus and E. coli are often present in blood cultures. (7) The cross-sectional and retrospective nature of this study is a limitation.…”

Section: Discussionmentioning

confidence: 99%

Sepse tardia em pré-termos de uma unidade de terapia intensiva neonatal: análise de três anos

Freitas¹,

Peloso²,

Manella³

et al. 2012

Rev. bras. ter. intensiva

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“…The research is particularly relevant in Brazil due to the high prevalence of E. coli in the country. Studies published by Freitas et al, (2012) and Pinheiro et al, (2009) mentioned neonatal infection rates between 28.5% and 30.6% for Grampositive and Gram-negative bacteria. The DNA amplification of E. coli by PCR is a sensitive, specific and easily performed technique, and does not require cell culture.…”

Section: Discussionmentioning

confidence: 99%

Neonatal sepsis: evaluation of risk factors and histopathological examination of placentas after delivery

Duarte¹,

Marcon²,

Akagi³

et al. 2019

Biosci. J.

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Neonatal sepsis is a clinical syndrome defined by systemic signs of infection in newborns accompanied by bacteremia. Can be responsible for serious consequences for the newborn child, characterized at the birth as early sepsis or late onset sepsis, with high rate of neonatal morbidity and mortality. Pathological agents such as Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Ureaplasma urealyticum and Mycoplasma hominis are most often responsible for intrauterine infections. The objective of this study is to evaluate the factors of neonatal sepsis predisposition in pregnant women through histopathological examination and the apoptotic index of placental tissues and detect DNA of E. coli and S. agalactiae using the Polymerase Chain Reaction (PCR). Histopathological analyses were made and the apoptotic index was determined to verify the levels of possible inflammatory infiltrates and cell death. Placenta samples were collected from November 2013 to May 2014. After DNA extraction, a PCR was performed amplifying the target fragment from the conserved regions of the rpoB (beta-RNA polymerase) polymorphism of E. coli and the factor 1 of S. agalactiae. The apoptosis index was tested with Acridine Orange and the histological procedure with Hematoxylin-Eosin staining. Among 100 samples of placental tissues analyzed by PCR, 48 represented the control group and did not present a risk factor associated with neonatal sepsis, and 52 samples representing the study group had at least one risk factor. Among these 52 samples, 7 (13.4%) had a PCR positive for E. coli. No placenta samples showed a positive PCR for S. agalactiae. The quantification of the apoptotic index did not show statistical significances between the groups and no inflammatory infiltrates were observed. However, histological sections showed fibrinoid necrosis, infarct areas and areas of calcification in all samples. Therefore, the results allow to conclude that the seven patients of experimental group with positive PCR for E. coli had eminent risk factors of neonatal sepsis, and the infection of the urinary tract (UTI) is the main aggravating circumstance. The histopathological examination demonstrated that the risk factors caused significant alterations, producing fibrinoid necrosis and infarcted areas in the placenta, contrary to apoptotic index that didn't differ from the group with unprecedented risk.

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Infecção hospitalar em Unidade de Terapia Intensiva Neonatal: há influência do local de nascimento?

Cited by 18 publications

References 29 publications

Outbreak of neonatal infection by an endemic clone of Serratia marcescens

Outbreak of neonatal infection by an endemic clone of Serratia marcescens

Sepse tardia em pré-termos de uma unidade de terapia intensiva neonatal: análise de três anos

Neonatal sepsis: evaluation of risk factors and histopathological examination of placentas after delivery

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