Inducing and identifying artificially-induced polyploidy in bananas

Pio, Leila Aparecida Salles; Pasqual, Moacir; Silva, Sebastião de Oliveira e; Rocha, H. S.; Magalhães, Hélida Mara; Santos-Serejo, Janay Almeida dos

doi:10.5897/ajb2014.14009

Cited by 9 publications

(9 citation statements)

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“…Moreover, oryzalin induces chromosome doubling at concentrations lower than those used for colchicine owing to the high-affinity binding of dinitroanilines to tubulin and the stability of the oryzalin-tubulin complex [11,28]. In the present study, the concentrations of the antimitotic agent tested appear to have been very low and ineffective for chromosomes doubling (Table 3).…”

Section: Resultsmentioning

confidence: 54%

“…These parameters were also reported in other species, with artificial polyploidization, such as Musa spp. [28], rangpur lime (Citrus limonia) [1], Brassica campestris [18] and citrus [19]. Cattleya tigrina presents endopolyploidy, with cells of three different ploidies in the same tissue in their natural environment.…”

Section: Resultsmentioning

confidence: 99%

“…Plant ploidy can be safely determined in the flow cytometric analysis since the DNA content is not influenced by external factors, such as water content in the plant tissue, leaf blade development, and light intensity [28]. Another methodology used to identify chromosome number counts is cytogenetics as seen in Dendranthema nankingense [23] and Lilium leichtlinii [15].…”

Section: Resultsmentioning

confidence: 99%

“…Stomatal analysis is a method that allows the identification of polyploid and diploid plants via counting and comparative verification of stomata, since the length of the stomata increases with the number of chromosomes [6,28]. The increase in the ploidy level is directly proportional to the size and number of the stomata.…”

Section: Resultsmentioning

confidence: 99%

“…Two hundred and forty in vitro C. tigrina plants, were subject to treatments with colchicine (C22H25NO6) and oryzalin (3,5-dinitro-N4, N4-dipropylsulfanilamide). Colchicine and oryzalin were tested at concentrations of 0, 2.5, 7.5, and 12.5 mM, 0, 10, 30, and 50 μM, respectively [28]. Inoculation was performed in 125 ml Erlenmeyer flask with 30 ml of MS liquid medium under agitation (60 rpm), for 24 and 48 hours for colchicine and three and six days for oryzalin, using 1000 ml Erlenmeyer flasks with 200 ml of MS liquid medium, with constant air bubbling by domestic aquarium air pumps.…”

Section: In Vitro Polyploidy Inductionmentioning

confidence: 99%

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Chromosome doubling in Cattleya tigrina A. Rich

et al. 2019

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Chromosome doubling induction in orchids may benefit their production for resulting in flowers of higher commercial value, larger size and higher content of substances that intensify the color and fragrance when compared with diploid orchids. This work aimed to induce and confirm artificial polyploidization, using flow cytometry and stomatal analysis. Explants were treated with colchicine at concentrations of 0, 2.5, 7.5, and 12.5 mM, for 24 and 48 hours and with oryzalin, at concentrations of 0, 10, 30, and 50 μM, for three and six days. For the flow cytometric analysis, a sample of leaf tissue was removed from each plant, crushed to release the nuclei and stained with propidium iodide. In addition to flow cytometry, the ploidy of the antimitotic treated plants was evaluated by stomata analysis. Young leaves were used where the density, functionality and stomatal index were evaluated. Colchicine provided induction of satisfactory polyploidy in C. tigrina at all concentrations and times of exposure, obtaining a greater number of polyploid individuals in the concentration of 12.5 mM for 48 hours. Oryzalin did not induce chromosome duplication at the tested concentrations.

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Section: Resultsmentioning

confidence: 54%