In vitro toxicological assessment of Arrabidaea brachypoda (DC.) Bureau: Mutagenicity and estrogenicity studies

Abstract

Arrabidaea brachypoda (DC.) Bureau is a shrub native Cerrado, known as "cipó-una", "tintureiro" or "cervejinha do campo" and popularly used in Southeastern and Northeastern Brazil to treatment of kidney stones and painful joints (arthritis). Nevertheless, scientific information regarding this species is scarce, and there are no reports related to its possible estrogenic and mutagenic effects. Thus, the principal objective of this study was to assess the mutagenic and estrogenic activities of the hydroalcoholic extracts of the leaves, stalks, roots, their respective fractions and isolated compounds of A. brachypoda. The mutagenic activity was evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, in the absence (-S9) and presence (+S9) of metabolic activation system. In the RYA was used Saccharomyces cerevisiae engineered strain BY4741 (MATaura3Δ0 leu2Δ0 his3Δ1 met15Δ0) which reproduce the natural pathway of genetic control by estrogens in vertebrate cells; it has the advantage of its simplicity and a high throughput. All extracts and aqueous fraction of leaves A. brachypoda were mutagenic. The crude extract is more active than the fraction, suggesting a synergic effect. Only hydroalcoholic extracts of leaves and roots of A. brachypoda showed significant estrogenic activity, with ERα-dependent transcriptional activation activity. The obtained results in this study showed the presence of compounds capable of interacting with the estrogen receptor and to induce damage in the genetic material. Thus, we demonstrated the risk which the population is subjected due to indiscriminate use of extracts without detailed study.

Fridericia platyphylla (Cham.) L.G. Lohmann root extract exerts cytotoxic and antiproliferative effects on gastric tumor cells and downregulates BCL-XL, BIRC5, and MET genes

“…41 Previously, mutagenic activity was reported for the same FP extract in Salmonella typhimurium strains TA98, TA97a, TA100, and TA102 with and without metabolic activation. 42 This test detects point mutations, compared with chromosome aberrations detected by CBMN-cyt, and can provide results that reinforce the mutagenic activity of the extract.…”

PROSPECÇÃO CIENTÍFICA E TECNOLÓGICA DE PRODUTOS DA ESPÉCIE Arrabidaea brachypoda (DC.) BUREAU

“…brachypoda estão provavelmente implicados no potencial mutagênico e estrogênico observado por Resende et al, (2017), tendo em vista que os flavonoides que apresentam atividade mutagênica, possuem em suas estruturas: hidroxila livre na posição 3 do anel C, dupla ligação entre as posições 2 e 3 e grupo ceto na posição 4 (RIETJENS et al, 2005, RESENDE et al, 2012. Algumas dessas características estruturais são exibidas por quatro flavonóides isolados da cera epicular por Alcerito et al (2002), o que explica os efeitos tóxicos observados (SILVA et al, 2000).…”

“…Mediante a isso, atualmente já existem debates sobre a exploração sustentável da biodiversidade brasileira, mesmo em uma era pós-genômica. Esse tema mostra-se recorrente e tem destaque mundial na busca de novos fármacos (RESENDE, 2017).…”

Avaliação do extrato floral de Fridericia platyphylla (Cham.) L. G. Lohmann sobre Candida krusei, C. tropicalis, C. guilliermondii e C. albicans

“…No estudo de Serpeloni et al (2020) os pesquisadores demonstraram potencial citotóxico com atividade anti-tumoral em células incubadas de tumores gástricos. Outras importantes atividades são observadas para F. platyphylla como no tratamento da artrite Carlini, 2005), antimicrobiano na inibição de Staphylococcus aureus e Escherichia coli, bem como, atividade tripanossomicida para Trypanossoma cruzi e leishmanicida Leishmania amazonensis (Resende et al, 2017), e antifúngica sobre Candida albicans (Sousa Andrade et al, 2020).…”