In vitro selection of salt tolerant cell lines in Solanum tuberosum L.

Abstract

Cell lines able to grow on media containing 50, 100, 150 or 200 mM NaCl were established from potato callus cultures by direct recurrent selection or gradual selection. In callus subjected to direct selection only small clusters of cells survived on medium with 150 or 200 mM NaCl, whereas on 100 mM small cell portions appear necrotic. When cell lines were obtained by successive subcultures on media with increased concentrations of NaCl, salt-tolerant calli were more compact and developed a greenish colour free from necrotic areas. The response of calli lines grown on media with NaCl was compared to control line. The NaCl-tolerant calli showed a decrease in relative growth rate and water content, with higher reductions in the 150 mM tolerant callus. Lipid peroxidation was increased in 50 mM and 100 mM NaCl-tolerant calli, while in 150 mM tolerant callus remained similar to 100 mM values. There was a significant increase in ascorbic acid content in 100 mM and 150 mM NaCl-tolerant calli as compared to the 50 mM, that was twofold the value found in the control. Also, the contents of soluble and insoluble proteins increased in salt-tolerant lines. SDS-PAGE of soluble proteins showed the synthesis of specific polypeptides in the presence of NaCl in culture medium and the synthesis of a new polypeptide.

In Vitro Selection of Salt Tolerant Calli Lines and Regeneration of Salt Tolerant Plantlets in Mung Bean (Vigna radiata L. Wilczek)

“…However 200 mM NaCl tolerant calli obtained either by direct or indirect selection process did not sustained a regular growth on salt supplemented media, these calli suffered with time a decrease in growth and all callus tissues died when the culture period was prolonged beyond one month. A similar response was reported in salt tolerant potato calli lines (Queiros, et al, 2007) and in Chrysanthemum sp. (Hossain, et al, 2007).…”

“…And in several other plant species such as, canola (Tyagi & Rangaswamy, 1997, Jain, et al, 1991a finger millet, (Pius, et al 1993) Cotton (Gosset,t et al, 1994 Potato (Olmos & Hellin, 1996;Queiros, et al, 2007) rice (Dang & Nuguyen, 2003;Shankdhar et al, 2000) Sunflower (Davenport, et al, 2003) chilli (Venkataiah, et al, 2004) sugarcane (Gandonou, et al, 2006).…”

“…Plants exposed to saline environment suffer from ion excess or water deficit and oxidative stress linked to the production of reactive oxygen species (ROS), which cause damage to lipids, proteins and nucleic acids (Hernandez, et al, 2000). Oxidative stress is considered to be one of the major damaging factors in plant cells exposed to salinity (Gossette, et al, 1994;Hernandez, et al, 1995;Khan & Panda, 2002;Queiros et al, 2007). The process of salt response and tolerance has been studied at the whole plant level (Hasegawa et al, 2000;Jogeshwar, et al, 2006).…”

Transgenic potato overexpressing Arabidopsis cytosolic AtDHAR1 showed higher tolerance to herbicide, drought and salt stresses

“…The MV treatments were carried out by soaking plant leaf discs (12 mm in diameter) in 2 ml of distilled water or in 1 or 3 μM MV for 12 or 24 hours at 25°C under continuous light. For the drought and salt stress treatments, shoot parts (2 cm) from cultured WT and transgenic potato plants were excised and transferred to MS medium supplied with 5% (w/v) polyethylene glycol (PEG 6000) for drought stress (Gopal and Iwama 2007) or 50 mM NaCl for salt stress (Queirós et al 2007). ) and maintained for 3 weeks under controlled conditions (25°C, 16 h light, 8 h dark photoperiod).…”

In vitro Tissue Culture, a Tool for the Study and Breeding of Plants Subjected to Abiotic Stress Conditions

“…Therefore, the level of malonyldialdehyde, produced during peroxidation of membrane lipids, is often used as an indicator of oxidative damage [31]. It has been reported that selected callus lines of Solanum tuberosum subjected to NaCl showed an increase in lipid peroxidation in comparison with salt tolerant lines [32].…”