In Vitro Selection of Salt Tolerant Calli Lines and Regeneration of Salt Tolerant Plantlets in Mung Bean (Vigna radiata L. Wilczek)

Rao, Srinath; Patil, Prabhavathi

doi:10.5772/28389

Cited by 7 publications

(4 citation statements)

References 61 publications

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“…Reduction in biological criteria (callus induction and survival) is mainly due to drop in auxin level, inhibition of auxin synthesis and chromosomal aberrations or due to decline of assimilation mechanism. The present result was in accordance with the report of Ayolie et al (2011); Rao and Patil, (2012). The number of days taken for callus induction was presented in Table 1 and Fig.…”

Section: Resultssupporting

confidence: 93%

In vitro mutagenesis to improve salt tolerance in rice (Oryza sativa L.)

Thamodharan

Pillai²

2020

EJPB

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Rice is the most important food crop in the world which has been severely affected with salinity. Aim of this study was to improve the salt tolerant ability of two moderately susceptible rice cultivars (White ponni and BPT-5204) using Ethyl Methane Sulfonate under in vitro condition. In this experiment salt tolerant callus were recovered in seeds treated with 0.5% EMS cultured on MS media supplemented with different concentrations of NaCl and sea water (starts with 0%, 0.5%. 1.0% and 1.5% NaCl to 10% and 25% seawater). The extant of salt tolerance was determined by callus growth parameters like callus induction frequency, relative growth rate and regeneration frequency. The reduction in growth parameters were more at 25% (v/v) sea water and 1.5% (w/v) NaCl treated media. Significant number of plants (17.23% and 22.49% plantlets respectively) was obtained in callus recovered in 3hr and 5hr EMS treated seeds cultured on 25% sea water and 1.5% NaCl treated media. Proline is an important osmaticm accumulated more under salt stress to maintain the turgor pressure and protect the plants from extreme desiccation. Under higher salt concentration BPT-5204 accumulated the higher amount of proline than white ponni. The plantlets obtained from higher concentration of salt can be transferred to field for the selection of elite lines.

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Section: Resultssupporting

confidence: 93%

In vitro mutagenesis to improve salt tolerance in rice (Oryza sativa L.)

Thamodharan

Pillai²

2020

EJPB

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“…In contrast, the salt-adapted cell lines, transferred to media containing 150 mM NaCl concentration, showed a marked growth of the tissue. However, in this case, the tissue mass was lower compared to the callus cultured in a salt-free medium [39]. Similar results were obtained by comparing the growth of the potato calli, either exposed directly to 50-200 mM NaCl or gradually acclimated to increased NaCl concentrations.…”

Section: Discussionsupporting

confidence: 83%

Antioxidant Response in the Salt-Acclimated Red Beet (Beta vulgaris) Callus

Tyburski,

Mucha

2023

Agronomy

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Callus cultures initiated from red beet tubers were acclimated to 75 or 100 mM NaCl salinity by exposing them to gradually increasing NaCl concentrations. The acclimated callus lines displayed growth rates comparable to the control culture cultivated on the NaCl-free medium. Several antioxidant system components were analyzed to assess the role of the antioxidant defense in the acclimated callus’s ability to proliferate on salt-supplemented media. It was found that proline and ascorbate concentrations were increased in salt-acclimated callus lines with respect to the control line. On the other hand, glutathione concentration was unchanged in all tested callus lines. Total activities of the antioxidant enzymes, namely superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), and class III peroxidase (POX, EC 1.11.1.7) were increased in salt-acclimated cultures. The enzymatic components of the antioxidant systems were upregulated in a coordinated manner during the initial phases of the culture cycle when the increase in callus fresh mass occurs.

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“…In the same manner, the increased concentration of saline in the cultural medium had a significant role in reducing regeneration rate, where the salinity level of 14 dS m -1 showed the minimum regeneration rate for both cultivars. These results are stated in previous studies on several crops species, including rice (17,20), wheat (5), sugarcane (15) and peas (19).…”

Section: Regeneration Of Plantlets From Nacl Tolerant Callisupporting

confidence: 87%

Molecular evaluation of salt tolerance induced by sodium azide in immature embryos of two wheat cultivars

Mahmood¹,

Al-falahi²,

Hamedullah³

et al. 2019

JoBRC

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Background: The artificial induction of mutations represents an efficient tool in creating genetic variations. For this reason, the current study was carried out in the Plant Tissue Culture Laboratory/Genetic Engineering Institute to induce genetic diversity for salt tolerance in two local cultivars of bread wheat (Al-Iraq and Tamooz 2) using in vitro application of sodium azide (SA). Objective: Immature seeds from both cultivars subjected to 0, 0.5, 1.0 and 2.0 Mm of SA to determine the optimal concentration for developing novel mutants. Materials and methods: The optimal dose of SA mutagen was found to be 2.0 mM, resulting in a 42% reduction in callus fresh and dry weight. The developed callus was subjected to five different salinity levels using NaCl (6, 8, 10, 12 and 14 dS m-1). Results: Mutants showed a significant decrease in the percentage of regenerated plants under salinity stress conditions. The used SSR markers approved the genetic diversity between the original and the mutants of the two cultivars (Al-Iraq and Tamooz 2) growing under normal and salinity stress. The presences and the absence of some fragments was prominent in plants derived from immature embryos of the two cultivars tested in salinity conditions. The used SSR markers (cfd 9, cfd4, cfd1 wmc405, PYL5, HKT1, HVA1 and htk1) were so efficient in distinguishing between the original and tissue culture-derived plants. Furthermore, the experienced SA levels induced a higher rate of mutant alleles in cv. Conclusion: Al-Iraq with 19 mutant alleles than Tamooz 2 which showed only 13 mutant alleles. The current study represents an additional prove to the effectiveness of mutagens and tissue culture technique in developing novel variants with improved performance under stress conditions.

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In Vitro Selection of Salt Tolerant Calli Lines and Regeneration of Salt Tolerant Plantlets in Mung Bean (Vigna radiata L. Wilczek)

Cited by 7 publications

References 61 publications

In vitro mutagenesis to improve salt tolerance in rice (Oryza sativa L.)

In vitro mutagenesis to improve salt tolerance in rice (Oryza sativa L.)

Antioxidant Response in the Salt-Acclimated Red Beet (Beta vulgaris) Callus

Molecular evaluation of salt tolerance induced by sodium azide in immature embryos of two wheat cultivars

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