In vitro propagation of Notocactus magnificus

Abstract

Most commercially grown cacti can be easily propagated by seed and/or cuttings. A group of rare and endangered species does not fit into this category and is therefore a good candidate for in vitro propagation productions as a tool to overcome habitat and plant-destruction. The number of rare and endangered species of Cacti goes into about 100. Many show a low production and germination of seeds and plantlets are prone to damping-off, making the in vitro propagation a feasible alternative for the multiplication and conservation of their germplasm. The aim of the present investigation is to establish a protocol for the in vitro culture and plant regeneration of Notocactus magnificus, the blue cactus, a highly ornamental species, native to Brazil. The surface sterilization of the explants was achieved with immersion for 10 min in sodium hypochlorite solution for either seeds (0

Tissue culture of ornamental cacti

“…Concentrations of growth regulators in the growth medium used for cactus species range from 0, as in the case of in vitro culture of Mammillaria gracilis Pfeiff. (Balen et al, 2004), to low, intermediate or high levels of cytokinins, such as BA (4.4, 6.7, 8.8, 13.3 and 44.4 µM), employed alone in several species of Mammillaria (Mammillaria longimamma, Mammillaria stylothele), Turbinicarpus and Escobaria vivipara, or in combinations with an auxin like NAA (2.7-10.7 µM) or IAA (2.9-5.7 µM) with Notocactus (Mata Rosas et al, 2001;Dávila-Figueroa et al, 2005;Medeiros et al, 2006;Quiala et al, 2009). Growth media have been supplemented with Kin at 0, 4.6, 13.9, 22.3, 27.9 and 46.5 µM in the absence or presence of an auxin (IAA or NAA), and zeatin has been added at 4.6 and 22.3 µM for in vitro multiplication of species of the genera Pediocactus, Sclerocactus and Toumeya (Clayton et al, 1990).…”

Influência do substrato e do enraizamento na aclimatização de Melocactus glaucescens Buining & Brederoo propagados in vitro

“…Esses resultados discordam dos encontrados por Medeiros et al (2006), que não observaram crescimento dos brotos não enraizados de Notocactus magnificus e apenas 20% dos brotos enraizados sobreviveram no ambiente ex vitro.…”

“…kotschoubeyanus, C. mínima, F. acanthodes, Mammillaria carmenae, N. magnificus, O. denegrii, P. robinii, T. laui (Ault & Blackmon, 1987;Malda et al, 1999;Mata-Rosas et al, 2001;Moebius-Goldammer et al, 2003;Medeiros et al, 2006;Retes-Pruneda et al, 2007;Quiala et al, 2009).…”

Morfogénesis in vitro de Mammillaria plumosa Weber

“…Para el establecimiento de un sistema de propagación in vitro en cactáceas se pueden emplear diversos tejidos u órganos como explantes, cuya elección depende del género y la especie. Se pueden usar brotes terminales de plántulas (Gómez et al, 2006), secciones laterales o verticales de cladodios (Estrada et al, 2002); aréolas simples (Pérez y Dávila, 2002;Ramírez et al, 2007;Quiala et al, 2009);semillas (Dávila et al, 2005;De Madeiros et al, 2006) y brotes florales (Wyka et al, 2006), entre otros, los cuales son cultivados con diferentes reguladores de crecimiento (auxinas, citocininas y giberelinas) de acuerdo con los objetivos de estudios y las respuestas morfogénicas deseadas.…”

“…Terminal shoots of seedlings can be used (Gómez et al, 2006), lateral or vertical sections of cladodes (Estrada et al, 2002); simple areolas (Pérez y Dávila, 2002;Ramírez et al, 2007;Quiala et al, 2009);seeds (Dávila et al, 2005;De Madeiros et al, 2006) and flower buds (Wyka et al, 2006), among others, which are grown with different growth regulators (auxins, cytokinins and gibberellins) in accordance with the study objectives and desired morphogenic responses.…”