In vitro propagation of endemic and endangered Muscari mirum from different explant types

Nasırcılar, Ayşe Gül; Mirici, Semra; Özgül, Karagüzel; Eren, Özkan; Baktir, İbrahim

doi:10.3906/bot-0907-90

Cited by 12 publications

(2 citation statements)

References 15 publications

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“…An efficient in vitro regeneration of N. tazetta "Chinensis" through twin-scale explants with basal plate cultured on MS medium supplemented with 1 mg l -1 IBA + 1 mg l -1 triiodobenzoic acid (TIBA) and activated charcoal has been reported (Abdel-Rahman et al 2017). The highest proliferation bulbs of Muscari mirum Speta has also been reported from four-scale explants cultured on MS medium containing 4 mg l -1 BAP and 0.25 mg l -1 NAA (Nasircilar et al 2011). Rice et al (2011) reported the highest bulblet induction from twin-scale explants of Brunsvigia undulata F.M.…”

Section: Discussionmentioning

confidence: 93%

“…In vitro organogenesis can be affected by several factors such as plant genotype, storage conditions, explant type, polar orientation of the explants, photoperiod, temperature, composition of culture medium, and PGRs ( Chen and Ziv 2005;Abdel-Rahman et al 2017;Begum et al 2019;Cheesman et al 2010;García-Fortea et al 2020;Nasircilar et al 2011;Sairam et al 2003). Photoperiod and concentration of PGRs are probably the most important influencing factors on in vitro organogenesis in the bulbous plants.…”

Section: Discussionmentioning

confidence: 99%

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Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Khonakdari

Rezadoost

Heydari

et al. 2020

Plant Cell Tiss Organ Cult

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Narcissus tazetta L., a bulbous plant belongs to the Amaryllidaceae family, contains alkaloid galantamine (GAL) with acetylcholinesterase inhibitory activity which has been recently considered to treat Alzheimer's disease (AD). In the current work, the effect of photoperiod (16/8 h light/dark and 24 h dark) and various concentrations of NAA, BAP, and GA 3 (0, 0.5, 1 and 2 mg l -1 ) on the in vitro mass bulblet regeneration of N. tazetta was studied. The GAL production ability of the regenerated bulblets was assessed by HPLC-UV-MS. Light treatments significantly affected the number of bulblet and leaf, the ratio of bulblet/leaf, and leaf length. The maximum number of bulblet (31.0 ± 1.58) and leaf (13.3 ± 1.33) was recorded from the cultures fortified with NAA and BAP (2 mg l -1 ) kept in 16/8 h light/dark, while the maximum leaf length (2.1 ± 0.92 cm) was measured on the MS medium containing 0.5 mg l -1 NAA and 2 mg l -1 BAP incubated in the same photoperiod. The average ratio of bulblet proliferation per explant was significantly different between studied photoperiod (1.1 ± 0.86) and 24 h dark (0.62 ± 0.31). The regenerated bulblets contained 40 and 20 µg g -1 DW GAL underexposed photoperiod and 24 h dark, respectively. This information could be useful in the commercial production of GAL as a valuable anti-AD compound through in vitro mass bulblet proliferation of N. tazetta. Key messageThe regenerated mass bulblets of Narcissus tazetta (Amaryllidaceae) on MS medium containing 2 mg l -1 NAA and BAP kept in 16/8 h light/dark are recommended to produce galanatamine and lycorine.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Khonakdari

Rezadoost

Heydari

et al. 2020

Plant Cell Tiss Organ Cult

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Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species

Muraseva

Novikova

2018

Rend. Fis. Acc. Lincei

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The Use of Biotechnology in Conservation of Plant Biodiversity and Studies in Turkey

Bürün

2021

Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji

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Doğa, çeşitli faktörlerin etkisi ile tahrip edilmekte bu da biyoçeşitlilik kayıplarına neden olmaktadır. Biyoçeşitlilik kayıplarının dünyada ciddi bir problem olması nedeniyle Uluslararası Doğayı Koruma Birliği (IUCN) bu konuda birtakım çalışmalar yapmaktadır. Biyoçeşitlilik kayıplarını önlemek için in situ ve ex situ koruma stratejileri geliştirilmiştir. Ex situ korumada, tohum gen bankaları ve arazide bitki koleksiyonları uygulamaları gibi geleneksel yöntemlerin yanısıra modern yöntemlerin de önemli bir yeri vardır. Özellikle yok olma tehlikesi altında olan türlerin korunmasında ex situ koruma stratejileri kapsamında in vitro teknolojinin kullanılması önemli avantajlar sunmaktadır. Bitki materyalini toplamada, çoğaltmada, uluslararası değişimde ve muhafaza etmede in vitro tekniklerden yararlanılmaktadır. Bu derlemede, in vitro teknikler kullanarak bitki biyoçeşitliliğin korunması ve Türkiye'deki çalışmalar sunulmuştur.

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In vitro propagation of endemic and endangered Muscari mirum from different explant types

Cited by 12 publications

References 15 publications

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species

The Use of Biotechnology in Conservation of Plant Biodiversity and Studies in Turkey

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