Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species

Muraseva, Dinara S.; Novikova, Tatyana

doi:10.1007/s12210-018-0693-8

Cited by 13 publications

(17 citation statements)

References 23 publications

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“…Although the effect of the genotype and the size of the explant on the bulb regeneration is confirmed, but the effect of NAA-cytokinin composition on the efficient production of bulbs in some other bulbous plants such as Iris sanguinea (Wang et al 2018), Lachenalia montana (Aremu et al 2015), Fritillaria ruthenica Wikstr. (Muraseva and Novikova 2018), Muscari muscarimi (Ozel et al 2015), and L. aestivum (Ptak et al 2013) have also been reported. The increasing effect of paclobutrazol and sucrose levels on the bulblet induction of L. aestivum in culture condition in vitro has been reported (Ptak et al 2013).…”

Section: Discussionmentioning

confidence: 93%

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Khonakdari

Rezadoost

Heydari

et al. 2020

Plant Cell Tiss Organ Cult

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Narcissus tazetta L., a bulbous plant belongs to the Amaryllidaceae family, contains alkaloid galantamine (GAL) with acetylcholinesterase inhibitory activity which has been recently considered to treat Alzheimer's disease (AD). In the current work, the effect of photoperiod (16/8 h light/dark and 24 h dark) and various concentrations of NAA, BAP, and GA 3 (0, 0.5, 1 and 2 mg l -1 ) on the in vitro mass bulblet regeneration of N. tazetta was studied. The GAL production ability of the regenerated bulblets was assessed by HPLC-UV-MS. Light treatments significantly affected the number of bulblet and leaf, the ratio of bulblet/leaf, and leaf length. The maximum number of bulblet (31.0 ± 1.58) and leaf (13.3 ± 1.33) was recorded from the cultures fortified with NAA and BAP (2 mg l -1 ) kept in 16/8 h light/dark, while the maximum leaf length (2.1 ± 0.92 cm) was measured on the MS medium containing 0.5 mg l -1 NAA and 2 mg l -1 BAP incubated in the same photoperiod. The average ratio of bulblet proliferation per explant was significantly different between studied photoperiod (1.1 ± 0.86) and 24 h dark (0.62 ± 0.31). The regenerated bulblets contained 40 and 20 µg g -1 DW GAL underexposed photoperiod and 24 h dark, respectively. This information could be useful in the commercial production of GAL as a valuable anti-AD compound through in vitro mass bulblet proliferation of N. tazetta. Key messageThe regenerated mass bulblets of Narcissus tazetta (Amaryllidaceae) on MS medium containing 2 mg l -1 NAA and BAP kept in 16/8 h light/dark are recommended to produce galanatamine and lycorine.

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Section: Discussionmentioning

confidence: 93%

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Khonakdari

Rezadoost

Heydari

et al. 2020

Plant Cell Tiss Organ Cult

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“…For example, BAP was successfully used for in vitro propagation of Gladiolus species and varieties (Hus sey 1977; Akhare et al 2008), and BAP in combination with auxins was used to propagate Muscari mirum Speta (Nasircilar et al 2011), Iris stenophylla Hausskn & Siehe ex Baker subsp. allisonii B. Mathew (Nasırcılar et al 2011), and species of the genus Fritillaria (Kulkhanova et al 2015, Muraseva et al 2015, Muraseva & Novikova 2018.…”

Section: Discussionmentioning

confidence: 99%

“…No me thods of Eranthis propagation in vitro has been developed pre viously. How ever, many studies report the potential of this method for the propagation and conservation of geo phytes (Ziv & Lilien-Kipnis 2000, Ozcan et al 2007, Cıg & Bas do gan 2015, Podwyszynska 2015, Muraseva & Novikova 2018.…”

Section: In Vitro Propagation Of Eranthis Stellata (Ranunculacea) Endemic Species With Narrow Distribution In the Russian Far East Northementioning

confidence: 99%

“…Nevertheless, a num ber of studies have shown that the use of aboveground organs, such as floral elements, leaves, and shoots, is more pre fe rable for introducing rare and endangered plant spe cies into culture. Using these parts helps to avoid complete removal of the mother plant from natural habitats (Ziv & Lilien-Kipnis 2000, Muraseva & Novikova 2018 and to pre vent the high contamination that is characteristic of under ground organs (Seyring 2002).…”

Section: In Vitro Propagation Of Eranthis Stellata (Ranunculacea) Endemic Species With Narrow Distribution In the Russian Far East Northementioning

confidence: 99%

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In vitro propagation of Eranthis stellata (Ranunculacea), endemic species with narrow distribution in the Russian Far East, Northeast China and North Korea

Эрст¹,

Erst²

2020

Bot Pac

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Eranthis stellata (Ranunculaceae) was cultured in vitro for the first time, for which the use of peduncles with underdeveloped flower buds as primary explants was found to be effective. Upon introduction to culture in vitro, explants should be cultured in the dark at low temperature (17°C) to prevent phenolic oxidation. A culture me dium containing ½ MS supplemented with 5 μM 6-benzylaminopurine is optimal for the propagation of this species. A morphogenic response rate of 32 % was achieved, and the propagation rate amounted to 4.2 shoots per explant. K e y w o r d s : in vitro culture, Eranthis stellata, micropropagation, conservation Р Е З Ю М Е Эрст А.А., Эрст А.С. Размножение в культуре in vitro Eranthis stellata (Ranunculaceae), эндемичного вида с ограниченным распространени ем на Дальнем Востоке России, СевероВостоке Китая и в Северной Корее. Впервые введен в культуру in vitro Eranthis stellata, сем. Ranunculaceae. Показана эффективность использования в качестве первичных эксплантов цветоносов с недоразвитыми бутонами. Отмечено, что на этапе введения в культуру in vitro экспланты необходимо культивировать при пониженной температуре (+17°C) в темноте для преодоления фенольного окисления. Питательная среда ½ MS, дополненная 5 µM 6-бензиламинопукина, является оптимальной для размножения данного вида: морфогенный ответ составил 32 %, ко эф фициент размножения 4.2 шт./эксплант.

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“…The production of medicinal species by the conventional methods seems to be highly laborious. Micropropagation is an alternative efficient system, which has been widely used by commercial nurseries and institutes for rapid plant production, germplasm conservation, elimination of pathogens, and for phytochemical screening of medicine-useful compounds (Teixeira da Silva and Dobranszki 2016; Muraseva and Novikova 2018). Plantlets obtained in vitro mostly are lacking the problems of bacterial or fungal infections ordinarily associated with the long-term maintenance of Begonia clonal stock (Murugan et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Leaf pigments and concentrations of phenolic compound in Begonia grandis plantlets obtained from the floral explants

Karpova

Nabieva

Fershalova

2021

Rend. Fis. Acc. Lincei

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The objective of this study was to design a protocol of the successful establishment of plants of Begonia grandis dry. in culture in vitro, and to evaluate the concentrations of chlorophyll a and b, carotenoids, anthocyanins and phenolic compounds, as well as the ratios of chlorophyll a/b, total chlorophyll/carotenoids and total chlorophyll/anthocyanins in the in vitro plantlets in comparison to the same parameters assessed in the leaves of greenhouse plants. A significant increase in the percentage of shoot formation (22.0 ± 1.5%) was recorded on the modified N6 medium supplemented with plant growth regulators in comparison with the standard breeding technology. Begonia grandis plantlets possessed the elevated contents of all photosynthetic pigments per unit mass of plant compared to the greenhouse plants. The increase in chlorophyll b was most substantial. The average anthocyanin concentrations in the plantlets and greenhouse plants were approximately equal, but the essential variation within B. grandis microclones was observed. Phenolic profiles of in vitro plantlets and greenhouse plants was rather similar. Concentrations per unit mass for the majority of components (especially organic and phenolic acids) in the plantlets increased in comparison to those in greenhouse plants (47.23 and 38.22 mg g −1 total phenolics, respectively). Overall data suggest that this protocol can be used for the production of a large number of plants of B. grandis in a good physiological state and with high phenolic content.

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Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species

Cited by 13 publications

References 23 publications

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

Effect of photoperiod and plant growth regulators on in vitro mass bulblet proliferation of Narcissus tazzeta L. (Amaryllidaceae), a potential source of galantamine

In vitro propagation of Eranthis stellata (Ranunculacea), endemic species with narrow distribution in the Russian Far East, Northeast China and North Korea

Leaf pigments and concentrations of phenolic compound in Begonia grandis plantlets obtained from the floral explants

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