In vitro micropropagation from nodal segments of Cleistanthus collinus

Quraishi, Afaque; Koche, Vijaya; Mishra, Sunita

doi:10.1007/bf00043433

Cited by 27 publications

(15 citation statements)

References 6 publications

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“…This may be due to residual cytokinin present in the shoots at multiplication stage. Quraishi et al (1996) (Rajeswari and Paliwal 2006), Boswellia ovalifoliolata (Chandrasekhar et al 2005) and Oroxylum indicum (Dalal and Rai 2004). In order to increase shoot multiplication rate, adjuvant were included in the MS medium fortified with 0.44 lM BAP.…”

Section: Resultsmentioning

confidence: 99%

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

Shukla

Mishra

2008

Trees

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Padar (Stereospermum personatum, family Bignoniaceae) is a well-known medicinal tree. Its complete regeneration occurred through shoot bud culture in vitro. The seeds germinated sequentially on plastic trays and polyethylene bags for 21 days served as explants source. Nodal segments from the seedlings were established on MS medium supplemented with 4.44 lM BA, in which 86.6% nodes showed shoot bud elongation. Then, nodal segments from the developed shoots were cultured on MS medium with several BA concentrations; best shoot multiplication was obtained with 0.44 lM BA. In a second experiment where PVP was added to proliferation medium, nodal segments from developed shoots produced maximum 2.78 shoots per node. The nodal segments showed shoot multiplication up to seventh subculture on. Finally, shoots were rooted on MS medium with 2.46 lM IBA. The plants transferred to net pots containing coco-peat were acclimatized in green house, where more than 80% plants survived and grew normally.

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Section: Resultsmentioning

confidence: 99%

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

Shukla

Mishra

2008

Trees

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“…A maioria das brotações regeneradas em meio basal, suplementado com 10 µM de BAP, mediam entre 0,5 e 1,0 cm de comprimento, necessitando de subcultivo para meio de alongamento, antes da indução de raízes. Resultados semelhantes foram obtidos para Cleistanthus collinus, com brotações mais longas induzidas em meios de cultura acrescidos de 2,2 µM de BAP (QURAISHI et al, 1996). Trindade et al (1990) constataram que níveis baixos de BAP (0,4 e 1,1 µM), combinados com concentração elevada de auxina (2,5 µM de AIB), estimularam a multiplicação in vitro de Eucalyptus globulus, e o efeito das auxinas provavelmente foi compensar os níveis baixos de citocininas.…”

Section: Indução De Brotações Múltiplasunclassified

Micropropagação de Aspidosperma polyneuron (peroba-rosa) a partir de segmentos nodais de mudas juvenis

Ribas¹,

Zanette²,

Kulchetscki³

et al. 2005

Rev. Árvore

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O presente trabalho teve como objetivo o estabelecimento de um protocolo de regeneração in vitro de mudas de Aspidosperma polyneuron (peroba-rosa), a partir de segmentos nodais de material juvenil. Brotações apicais de mudas de dois anos de idade foram desinfestadas com 0,25% de hipoclorito de sódio ou 0,05% de cloreto de mercúrio, durante 10 min, visando ao estabelecimento de culturas assépticas. A indução de brotações múltiplas foi realizada em meio de cultura WPM, suplementado com BAP, ZEA ou CIN (2,2-8,8 miM), no cultivo inicial e nos dois subcultivos subseqüentes. Para indução de brotações alongadas foram testadas as combinações de fitorreguladores: 2,25 miM de BAP, ZEA ou CIN, associadas com 1,25 miM de AIB. A indução de raízes foi avaliada com tratamentos em soluções de AIB (2,5-10 mM), durante 5 ou 15 min. As mudas enraizadas foram transplantadas para casa de vegetação. A desinfestação das brotações apicais foi eficiente com 0,25% de NaOCl ou 0,05% de HgCl2, durante 10 min, obtendo-se 72,89 e 84,10% de sobrevivência, respectivamente. As maiores taxas médias de regeneração de brotações axilares (4 a 5) foram obtidas em meio de cultura suplementado com ZEA ou BAP (4,4-8,8 miM), após o segundo subcultivo. Concentrações mais reduzidas de BAP ou ZEA (2,25 miM) e 1,25 miM de AIB proporcionaram, em média, três brotações mais alongadas (1,5-2,5 cm de comprimento). Tratamentos com soluções de 10 mM de AIB, durante 15 min, foram eficientes na indução de raízes (80%), e as mudas transplantadas apresentaram taxas de sobrevivência superiores a 90% em casa de vegetação.

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“…In general, micropropagation through axillary shoot proliferation has proven to be a handy tool [11] An appreciable level of success has been achieved by many investigators in the case of many tropical medicinal plants through micropropagation as in case of Hemidesmus indicus ([12], Cleistanthus collinus [13], Gmelina arborea [14], Plumbago zeylanica [15] and Holarrhena antidysenterica [16]. Although there are techniques have been reported for in vitro propagation of Aegle marmelos from in vitro grown seedling parts, i.e.…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> propagation of <i>Aegle marmelos</i> (L.) corr., a medicinal plant through axillary bud multiplication

Puhan¹,

Rath²

2012

ABB

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A protocol for rapid in vitro propagation from nodal explants of the medicinal tree species Aegle marmelos (L.) corr. of family Rutaceae has been described. High frequency bud break were induced on Murashige and Skoog's (1962) basal medium supplemented with 0.5 mg benzyladenine (BA)/l. After 10 days of culture, nodal explants with multiplied buds started callusing with restricted growth and defoliation. When the same nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with different concentrations of either kinetin (KN) or gibberellic acid (GA 3 ) or in combinations has shown healthy shoots with expanded shoot length. Excised shoots (2 cm -3 cm long with 2 to 3 nodes) when grown on 1/2 MS basal medium with 2.5 mg/l indole-3-butyric acid (IBA) and 0.5% activated charcoal (A.C.)/l has shown rhizogenesis. After excision, in the second passage, the nodal explants also showed bud break when sub cultured on MS basal medium supplemented with 0.5 mg BA/l. These shoots also successfully rooted on the same above said medium.

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In vitro micropropagation from nodal segments of Cleistanthus collinus

Abstract: Cleistanthus collinusBenth. was micropropagated using nodal explants on MS medium supplemented with 2.

Cited by 27 publications

References 6 publications

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

Micropropagação de Aspidosperma polyneuron (peroba-rosa) a partir de segmentos nodais de mudas juvenis

<i>In vitro</i> propagation of <i>Aegle marmelos</i> (L.) corr., a medicinal plant through axillary bud multiplication

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In vitro micropropagation from nodal segments of Cleistanthus collinus

Abstract: Cleistanthus collinusBenth. was micropropagated using nodal explants on MS medium supplemented with 2.

Cited by 27 publications

References 6 publications

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

Micropropagação de Aspidosperma polyneuron (peroba-rosa) a partir de segmentos nodais de mudas juvenis

&lt;i&gt;In vitro&lt;/i&gt; propagation of &lt;i&gt;Aegle marmelos&lt;/i&gt; (L.) corr., a medicinal plant through axillary bud multiplication

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<i>In vitro</i> propagation of <i>Aegle marmelos</i> (L.) corr., a medicinal plant through axillary bud multiplication