In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

Abstract

Padar (Stereospermum personatum, family Bignoniaceae) is a well-known medicinal tree. Its complete regeneration occurred through shoot bud culture in vitro. The seeds germinated sequentially on plastic trays and polyethylene bags for 21 days served as explants source. Nodal segments from the seedlings were established on MS medium supplemented with 4.44 lM BA, in which 86.6% nodes showed shoot bud elongation. Then, nodal segments from the developed shoots were cultured on MS medium with several BA concentrations; best shoot multiplication was obtained with 0.44 lM BA. In a second experiment where PVP was added to proliferation medium, nodal segments from developed shoots produced maximum 2.78 shoots per node. The nodal segments showed shoot multiplication up to seventh subculture on. Finally, shoots were rooted on MS medium with 2.46 lM IBA. The plants transferred to net pots containing coco-peat were acclimatized in green house, where more than 80% plants survived and grew normally.

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

“…In the present study, shoot tip and nodal segments obtained from two year old plants were used as explants. Better plant regeneration from node of young trees was also reported in Oroxylum (Dalal and Rai 2004), Boswellia ovalifoliolata (Chandrasekhar et al 2005), Pterocarpus santalinus (Rajeswari and Paliwal 2006), Stereospermum personatum (Shukla et al 2008). Browning of explants due to exudation of phenolics has been reported as a serious problem in establishing axenic cultures of tree species (Krishna and Singh 2007).…”

In vitro Regeneration of Multipurpose Medicinal Tree Stereospermum Suaveolens?Factors Controlling the In Vitro Regeneration

“…Thus, MS medium supplemented with 4.44 μM BAP was found to be the most suitable medium for establishment of nodal explants of S. suaveolens. In earlier studies also MS medium was found suitable for in vitro establishment of explants from hardwood trees such as Terminalia arjuna Roxb [11], Tectona grandis [12], Gmelina arborea [13], woody climber Bauhinia vahlii [14] and Stereospermum personatum [15]. Shoot bud elongation occurred when nodes were placed on MS medium supplemented with 0.0, 0.04, 0.44 or 4.44 µM BAP [16].…”

Review of Literature

“…Generally, IBA has been observed to induce strong rooting response over IAA or NAA and has been extensively used to promote rooting in a wide range of tree species such as Myrtus communis (Ruffoni et al 1994;Scarpa et al 2000), Pistacia vera (Onay 2000), Terminalia arjuna (Pandey and Jaiswal 2002;Pandey et al 2006), Terminalia chebula (Shyamkumar 2003), Kigelia pinnata (Thomas and Puthur 2004), Stereospermum personatum (Shukla et al 2009), Semecar-pus anacardium (Panda and Hazra 2010), and Sapindus trifoliatus (Asthana et al 2011). Moreover, this group of plant hormone is readily and easily available commercially worldwide (Epstein and Ludwig-Miller 1993).…”

“…The increase in shoot number may be due to the suppression of apical dominance during subculture that induced basal dormant meristematic cells to form new shoots (Shukla et al 2009). …”

“…Nodal segments have been widely used for in vitro shoot proliferation of woody plants such as Syzygium cuminii (Jain and Babbar 2000), Terminalia chebula (Shyamkumar et al 2003), Oroxylum indicum (Dalal and Rai 2004), Holarrhena antidysenterica (Kumar et al 2005), Boswellia ovalifoliolata (Chandrasekhar et al 2005), Tectona grandis (Shirin et al 2005), Pterocarpus santilinus (Rajeswari and Paliwal 2006), miracle berry (Ogunsola and Ilori 2007), Stereospermum personatum (Shukla et al 2009), Vitex negundo (Ahmad and Anis 2011), and Sapindus trifoliatus (Asthana et al 2011). This is probably due to the readily available axillary buds in nodal segments that only require a trigger for bud break in contrast to root, leaf, and cotyledonary tissue that would otherwise require initiation of adventitious buds (Lombardi et al 2007, Sivanesan et al 2007) and somatic embryos (Naz et al 2008, Husain et al 2010) before any shoot regeneration is achieved.…”