In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. &amp; Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe2+-induced lipid peroxidation in rat pancreas

Adefegha, Stephen A.; Oboh, Ganiyu

doi:10.1016/s2221-1691(12)60228-7

Cited by 77 publications

(56 citation statements)

References 37 publications

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“…Antioxidant activity in higher plants has often been associated with phenolic compounds [26] . In addition to their roles in plants, phenolic compounds in our diet may provide health benefits associated with reduced risk of degenerative diseases [6] .…”

Section: Discussionmentioning

confidence: 99%

“…In DPPH assay the lower the EC 50 the better it is able to scavenge the radicals, particularly peroxy radicals which are the propagators of the autoxidation of lipid molecules and there by break the free radical chain reaction [16] . Substances which are able to perform this reaction can be considered as antioxidants [26] . The result of DPPH agreed with earlier reports where plant phytochemicals scavenged DPPH [12,16,27] .…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro

Shodehinde

Oboh

2013

Asian Pacific Journal of Tropical Biomedicine

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro

Shodehinde

Oboh

2013

Asian Pacific Journal of Tropical Biomedicine

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“…These phenomena determined structure of phenolic compounds, such as free or bound phenolics. Many studies have informed that phenolic compounds of fruits and vegetables are usually in free structure higher than in bound structure [29,30]. There was a free form of phenolics of pluchea leaves and green tea in aqueous media showed that there was hydroxyl group free.…”

Section: Total Phenol Contentmentioning

confidence: 99%

In Vitro Antioxidant Capacities and Antidiabetic Properties of Pluchea Leaves and Green Tea Mixtures at Various Proportions

Widyawati

Werdani

Setiokusumo

2017

Int J Pharm Pharm Sci

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Objective: This study was done to determine the effect of pluchea leaves and green tea mixtures at various proportions to antioxidant and antidiabetic properties. Methods:The research used a single factor randomized block design. Research factors were pluchea and green tea mixture proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w), respectively. Each of factors was repeated five times. The parameters observed in this study were phytochemical compounds, total phenols, total flavonoids, free radical DPPH scavenging activity, iron ion reducing power, alpha amylase and alpha glycosidase inhibition activities. The data were statistically α = 5%, if the data analysis showed a significant analyzed using analysis of variance (ANOVA) at effect then it was continued with duncan's multiple range test (DMRT). Results:The phytochemical compounds containing in pluchea leaves and green tea mixtures were alkaloids, flavonoids, phenolics, saponins, tannins, triterpenoids, sterols, and cardiac glycosides. The qualitative assay of phytochemical compounds from aqueous extract had a different pattern with total phenolic content (TPC) and total flavonoid content (TFC), especially at 75:25; 50:50 and 25:75 % (w/w) from pluchea leaves and green tea mixture proportions. The 50:50% (w/w) proportions of samples had the highest TPC and TFC, it was proposed that there was an interaction between bioactive compounds of pluchea leaves and green tea. TPC and TFC were correlated with free radical DPPH scavenging activity, iron reducing power and alpha glycosidase inhibition activity, but the alpha amylase inhibition activity had the different graph pattern with the TPC and TFC. Conclusion:The antioxidant capacity of pluchea leaves and green tea mixtures at various proportions had the graph pattern similar to the alpha glycosidase inhibitory activity and different with the alpha amylase inhibitory activity.

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“…Lipid peroxidation in biological membranes is considered one of the major mechanisms of cell injury in aerobic organisms subjected to oxidative stress. The chain reaction of lipid peroxidation ensures the continuous supply of free radicals, which initiate further peroxidation (Adefegha and Oboh, 2012). The antioxidative assay conducted in this study indicates that OP has in vitro antioxidant activities.…”

Section: Resultsmentioning

confidence: 61%

In Vitro Antioxidant Activity and In Vivo Anti-fatigue Effects of Oyster (Ostrea plicatula Gmelin) Peptides Prepared Using Neutral Proteinase

Hao

Cao

Hao

et al. 2013

FSTR

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The amino acid profile of oyster (Ostrea plicatula Gmelin) peptides (OP) was assayed. Its in vitro antioxidant activity was determined, and its in vivo anti-fatigue activity was compared with those of oyster meat and oyster protein. Seven amino acids that may play an important role in enhancing antioxidant activity account for 25.8% of total OP content. These amino acids are histidine, proline, methionine, cysteine, tyrosine, tryptophan, and phenylalanine. The F-value of OP was 4.89, and the concentration of taurine was 45.43 mmol/mL. OP acted as a scavenger for hydroxyl radicals, 1,1-diphenyl-2-picrylhydrazyl, and superoxide anion radicals. It also inhibited lipid peroxidation. The intragastric administration of OP prolonged the swimming time to exhaustion of mice by 78% over that of the control. It decreased the levels of serum lactic acid and blood urea nitrogen by 24.8% and 11.2%, respectively. Finally, it increased the levels of liver glycogen (1.0-fold) and gastrocnemius muscle glycogen (55.6%).Keywords: oyster peptides, antioxidant activity, anti-fatigue effect, endurance exercise *To whom correspondence should be addressed. E-mail: zhangch2@139.com IntroductionAcute and/or chronic exercises are associated with increases in free radicals and ROS production, which are due primarily to the increase in oxygen uptake by active tissues (Ji, 1995;Powers and Jackson, 2008). The imbalance between free radical/ROS production and its removal by antioxidant defense systems causes oxidative stress (Chalamaiah et al., 2012). Oxidative stress may lead to potential damage and consequently diminish athletic performance. Thus, increased free radical/ROS production has been associated with altered muscle function and elevated rates of muscle fatigue (Deaton and Marlin, 2003).Research consensus reflects support for the idea that antioxidative peptides can reduce the damage induced by ROS and free radicals. The antioxidative peptides from aquatic products have become of recent concern because they are considered safe and healthy molecules with low molecular weight, easy absorption, low cost, and high activity (Sarmadi and Ismail, 2010). The precursor protein in these peptides is mostly inactive. These active peptides can also be released from the precursor protein by hydrolysis techniques, such as enzymatic hydrolysis, autolytic process using endogenous enzymes, and microbial fermentation (Chalamaiah et al., 2012;Je et al., 2007;Rajapakse et al., 2005;Kim et al., 2007).In this study, we assayed the amino acid profile of oyster peptides (OP), and then investigated its in vitro antioxidant activities and the in vivo anti-fatigue effects of OP previously prepared by neutral proteinase digestion. The in vitro antioxidant activities were studied by determining the activities of hydroxyl radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and superoxide anion radical scavengers, as well as lipid peroxidation inhibitory activity in a linoleic acid emulsion system. In the determination, glutathione (GSH) which is a well known peptide an...

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In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe2+-induced lipid peroxidation in rat pancreas

Cited by 77 publications

References 37 publications

Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro

Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro

In Vitro Antioxidant Capacities and Antidiabetic Properties of Pluchea Leaves and Green Tea Mixtures at Various Proportions

In Vitro Antioxidant Activity and In Vivo Anti-fatigue Effects of Oyster (Ostrea plicatula Gmelin) Peptides Prepared Using Neutral Proteinase

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