In vitro growth of Brassocattleya orchid hybrid in different concentrations of KNO3, NH4NO3 and benzylaminopurine

Cardoso, Jean Carlos; Ono, Elizabeth Orika

doi:10.1590/s0102-05362011000300017

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…Reducing MS media concentrations significantly increased PLBs formation of Grammatophyllum orchid (Sopalun et al, 2010) and Brassocattleya orchid (Cardoso and Ono, 2011). In contrast, some orchid such as Vanilla planifolia needed the rich salt content of full strength MS to enhance early stage of in vitro growth (Geetha and Shetty, 2000).…”

Section: Results and Discussion Effects Of Different Ms Strengths On mentioning

confidence: 99%

“…In contrast, some orchid such as Vanilla planifolia needed the rich salt content of full strength MS to enhance early stage of in vitro growth (Geetha and Shetty, 2000). Adjusting MS concentrations is essential for each plant species to obtain optimum in vitro growth and morphogenesis development (as reviewed by Cardoso and Ono, 2011). Most favourable MS salt provides sufficient nutrient required to promote metabolism and cell growth at the same time prevent toxic effect of media salt (Fadel et al, 2010).…”

Section: Results and Discussion Effects Of Different Ms Strengths On mentioning

confidence: 99%

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Efficient micropropagation of Dendrobium sonia-28 for rapid PLBs proliferation

Julkiflee

Uddain

Subramaniam

2014

Emir. J. Food Agric

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Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved in this study. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid culture. In sucrose treatment, the highest increased of PLBs growth rate percentage was in 10g.L -1 . PLBs growth rate percentage was the highest (14%) in media devoid of exogenous 6-benzylaminopurine (BAP) or naphthalene acetic acid (NAA). However, the addition of BAP and NAA in combinations of BAP (4.44 or 8.88 µM) and NAA (8.88 µM) resulted in increased PLBs growth rate to 14% compared to when added separately. The optimized micropropagation media and condition of PLBs proliferation are important for the production of large number of plant material required for the development of genetic modification and cryopreservation studies.

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Section: Results and Discussion Effects Of Different Ms Strengths On mentioning

confidence: 99%

Section: Results and Discussion Effects Of Different Ms Strengths On mentioning

confidence: 99%

Efficient micropropagation of Dendrobium sonia-28 for rapid PLBs proliferation

Julkiflee

Uddain

Subramaniam

2014

Emir. J. Food Agric

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“…It could be noted that Brazil has the highest number of published works both on propagation and germplasm conservation, while for some countries only researches referring to propagation of native orchid species are reported and for other countries (Guyana, Paraguay, Trinidad and Tobago, Suriname, Uruguay) there were no registered works in any of the topics. Almeida et al 2017;Caovila et al 2016;Cardoso and Ono 2011;Cavalcante Martini et al 2001;Corrêa Pereira et al 2011;2015;Da Silva et al 2014;Da Silva Júnior et al 2012;De Carvalho et al 2018;De Conti et al 2018;De Melo Ferreira et al 2017;De Menezes Gonçalves et al 2012;Dezan et al 2012;Dignart et al 2009;Dorneles and Trevelin 2011;Endres Júnior et al 2014;Favetta et al 2017;Galdiano Júnior et al 2011;2012a,b;Gomes de Araújo et al 2009;Hosomi et al 2017;Lando et al 2016;Lone et al 2010;Mantovani et al 2016;Massaro et al 2012;Monteiro do Rêgo et al 2009;Moraes et al 2005;Pasqual et al 2011;Pedroso-de-Moraes et al 2012;Pereira et al 2018;Pereira Gomes et al 2015;Prizão et al 2012;Rodrigues et al 2015;Rodrigues Guimarães et al 2013;Sasamori et al 2015;…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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“…No cultivo e subcultivo de plântulas dos gêneros Oncidium Sw., Laelia Lindl. e Cattleya Lindl., Unemoto et al (2007) ONO, 2011). O carvão ativado normalmente é adicionado ao meio de cultivo em concentrações de 0,2 a 3% (BEYL, 2000) e sua presença pode promover e/ou inibir o crescimento in vitro, dependendo da espécie e do tecido utilizados (PAN;van STADEN, 1998).…”

Section: Introductionunclassified