Micropropagação de híbridos de orquídea em meio knudson com adição de vitaminas do meio ms, benzilaminopurina e carvão ativado

Villa, Fabíola; Pasqual, Moacir; Silva, Enoque Fernandes da

doi:10.5433/1679-0359.2014v35n2p683

Cited by 18 publications

(14 citation statements)

References 5 publications

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“…Para os meios de cultura, não foram observadas diferenças para as variáveis comprimento médio de raízes e intensidade de calos, e esta última não foi influenciada, também, pela utilização de carvão ativado. Similarmente, Villa et al (2014) observaram que a adição de carvão ativado ao meio de cultura não promoveu a formação de calos na base dos explantes de amoreira-preta "Ébano" e porta-enxerto de videira "P1103".…”

Section: Resultsunclassified

Cultivo in vitro do cultivar italiano de morangueiro Pircinque

Camargo¹,

Meneguzzi²,

Rufato³

2020

ABC

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Estudos sobre a multiplicação de cultivares de morangueiro recentemente introduzidos no Brasil são necessários para verificar o potencial de produção das mudas. Verificaram-se dois meios de cultura (KNOP e MS) e componentes, no estabelecimento e multiplicação in vitro de Pircinque, a fim de otimizar o protocolo de micropropagação para o cultivar. Pesquisaram-se concentrações de PPM® durante o estabelecimento e na multiplicação, níveis de sacarose, ágar, carvão ativado, Fe EDDHa, BAP e GA3 . Além disso, foi avaliada a influência da “dupla fase”, com combinações do meio de cultivo, carvão ativado e BAP. Concluiu-se que, no estabelecimento, KNOP + 2 mL L-1 PPM possibilita maior sobrevivência dos explantes, enquanto o meio de cultura MS favorece a multiplicação deles. A adição dos seguintes componentes é indicada para a maior eficiência da técnica: 30 g L-1 sacarose; 6 g L-1 ágar; 4 g L-1 carvão ativado; 0,1 g L-1 Fe EDDHA; 1 mg L-1 BAP; e 0,250 mg L-1 GA3 . Após cultivo em meio MS sólido, indica-se o uso da técnica de “dupla fase”, com adição de MS líquido com a combinação de 0,5 mg L-1 de BAP e de 4 g L-1 de carvão ativado.

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Section: Resultsunclassified

Cultivo in vitro do cultivar italiano de morangueiro Pircinque

Camargo¹,

Meneguzzi²,

Rufato³

2020

ABC

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“…According to Villa et al (2014), this addition modifies the composition of the culture medium, adsorbs inhibitory substances produced by the medium itself and toxic substances (phenols and/or quinones) produced during the autoclaving process, or released by the explants, whose tissues underwent lesions, thus inhibiting the formation of calluses. Therefore, if indirect embryogenesis is the intention, the WPM medium is the most suitable for callogenesis in tamarind nodal segments without the use of activated charcoal.…”

Section: Resultsmentioning

confidence: 99%

Nodal segments and zygotic embryos in culture media for the in vitro propagation of tamarind

et al. 2019

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There has been a lack of research on the propagation methods of tamarind, hindering the availability and supply of its products. Considering the nutraceutical potential of tamarind and the long-time required to initiate production, this study aimed to evaluate the establishment of nodal segments and the germination of in vitro zygotic embryos of tamarind plants in culture media, salt concentrations, and activated charcoal, aiming at the establishment of an in vitro propagation protocol. For this purpose, MS and WPM media with 25, 50, 75 and 100% salt concentrations were used both with and without activated charcoal (2 g L-1) for the inoculation of nodal segments and zygotic embryos of tamarind. The experiment was conducted in a completely randomized experimental design, in a 2 x 4 x 2 factorial scheme (culture media x salt concentrations x activated charcoal) with 20 replications. It was observed that the in vitro establishment of tamarind nodal segments is viable when using the MS medium with 75% of salts plus 2 g L-1 of activated charcoal and that for the germination of zygotic embryos there was no influence of the treatments. It was also observed that in order to increase the length of the formed plantlets, the MS and WPM media can be employed with 75% of salts.

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“…The use of antioxidants (polyvinylpyrrolidone -PVP and activated charcoal) allowed lower oxidation and greater development of Terminalia amazonia explants during micropropagation (MÉNDEZ et al, 2014). The use of activated charcoal in the intergeneric hybrid of orchid Brassocattleya pastoral × Laeliocattleya amber accounted for the largest number of shoots (VILLA et al, 2014).…”

Section: Activated Charcoal In the In Vitro Multiplicationmentioning

confidence: 99%

“…Nevertheless, tissue culture technique has been helping to preserve these species, since they allow the management of a large number of individuals living in reduced spaces, under aseptic conditions (RIGHETO et al, 2012;VILLA et al, 2014;POOBATHY et al, 2019). Accordingly, micropropagation, from germination to the subsequent phases on in vitro culture, is an efficient propagation and conservation technique applied to the germplasm of orchid species (GOMES et al, 2015;HUNHOFF et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Souza

Fernandes

Molinari

et al. 2021

NAT

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Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

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Micropropagação de híbridos de orquídea em meio knudson com adição de vitaminas do meio ms, benzilaminopurina e carvão ativado

Cited by 18 publications

References 5 publications

Cultivo in vitro do cultivar italiano de morangueiro Pircinque

Cultivo in vitro do cultivar italiano de morangueiro Pircinque

Nodal segments and zygotic embryos in culture media for the in vitro propagation of tamarind

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

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