Efficient micropropagation of Dendrobium sonia-28 for rapid PLBs proliferation

Julkiflee, Advina Lizah; Uddain, Jasim; Subramaniam, Sreeramanan

doi:10.9755/ejfa.v26i6.18020

Cited by 9 publications

(6 citation statements)

References 18 publications

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“…The length of these roots was also highest in MS medium with 1.0 mg/L IBA (Table 4). The effect of IAA or IBA on induction of roots reported in other orchid species like Vanda tessellata , Rhynchostylis retusa (Bhattacharjee and Islam, 2015) and Dendrobium transparens (Sunitibala and Kishor, 2009;Julkiflee et al, 2014), Arundina graminifolia (Bhadra and Bhowmik, 2005). All the substrate covered with a layer of 10.0 gm sphagnum moss (Table 5).…”

Section: Discussionmentioning

confidence: 95%

In vitro pseudobulb based micropropagation for mass development of Cymbidium finlaysonianum Lindl.

Islam¹,

Islam²,

Bhattacharjee³

et al. 2015

Emir. J. Food Agric

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Section: Discussionmentioning

confidence: 95%

In vitro pseudobulb based micropropagation for mass development of Cymbidium finlaysonianum Lindl.

Islam¹,

Islam²,

Bhattacharjee³

et al. 2015

Emir. J. Food Agric

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“…In addition, Ng and Saleh (2011) suggested that PGR-free medium could obtain genetically stable PLBs. In recent years, secondary PLB development from PLBs has been shown for various orchids, including Dendrobium (Julkiflee et al, 2014), Doritaenopsis (Amaki and Higuchi, 1989), and Phalaenopsis (Huang et al, 2014;Khoddamzadeh et al, 2011). However, the results in this study showed that both large and small PLBs formed secondary PLBs (PLB proliferation) at nearly the same rates over 2, 4, and 6 weeks of culture (Fig.…”

Section: Resultsmentioning

confidence: 49%

“…Effects of PLB size and physical wounding on PLB proliferation. Julkiflee et al (2014) revealed that PGR-free medium could increase PLB proliferation. In addition, Ng and Saleh (2011) suggested that PGR-free medium could obtain genetically stable PLBs.…”

Section: Resultsmentioning

confidence: 97%

Micropropagation of Tolumnia Orchids through Induction of Protocorm-like Bodies from Leaf Segments

Chookoh¹,

Chiu²,

Chang³

et al. 2019

horts

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A protocol for plant regeneration via direct induction of protocorm-like bodies (PLBs) from leaf segments of Tolumnia Snow Fairy was developed as a basis for mass production. Ten-month-old, in vitro–grown donor plantlets were obtained by inducing shoots from buds on the flower stalk. Leaf segments harvested from plantlets of different heights and from expanding leaves at different positions were compared, as were two BA concentrations with 0.5 mg·L−1 NAA. The greatest rate of PLB induction (16.7%) was observed when leaf segments taken from 1- to 2-cm height plants were cultured in Murashige and Skoog (MS) basal medium supplemented with 2 mg·L−1 BA and 0.5 mg·L−1 NAA after 16 weeks of culture. When using leaf explants, only inner, expanding leaves cultured on MS basal medium supplemented with 4 mg·L−1 BA and 0.5 mg·L−1 NAA resulted in PLB induction, at an average rate of 25.5 PLBs per explant. After 16 weeks of culture, histological and scanning electron microscopy (SEM) observations revealed that PLBs originated from epidermal cells of leaf explants. PLBs of 1 to ≤2 mm in diameter continued to proliferate after 4 weeks of culture. These secondary PLBs could be produced from either whole PLBs or the upper side of PLBs. Finally, PLBs were regenerated into plantlets. After ≈14 months of culture, fully developed plants exhibiting well-developed roots and shoots were acclimatized. These plants grew well, with 1-year survival rates of nearly 73%, for plants originating as explants taken from 1- to 2-cm tall plants, and of 79%, for plants originating as explants taken from inner leaves. Some mature plants flowered 1 year after transplantation. This study presents a simple system that can provide a large number of PLBs for mass propagation in a short time that can be converted into plants and also used for the new cultivars of Tolumnia orchids.

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“…In culture duration of 10 weeks, the highest root number (6.1 ± 1.4) was recorded in medium supplemented with 3.0 mg/L KN and 1.5 mg/L NAA (Table 2). The stimulatory effect of auxins in rooting initiation and multiplication had previously been reported in several orchid species [32–35]. The seedlings after 10 weeks were subcultured to similar culture conditions with same plant growth regulator combinations.…”

Section: Resultsmentioning

confidence: 99%

Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

Nongdam

Tikendra

2014

The Scientific World Journal

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An efficient in vitro regeneration protocol from seed culture has been established successfully for Dendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values. Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with 0.4% activated charcoal (AC), 2 mg/L 6-benzyl amino purine (BAP), and 2 mg/L indole-3-acetic acid (IAA) produced best seed germination percentage in 2 weeks of culture. Incorporation of higher concentration of kinetin (KN) or BAP in combination with low auxin in medium induced pronounced shooting and leaf formation. Reduction in leaf development was evident when cytokinins exist singly in medium indicating synergistic effect of auxin and cytokinin in leaf induction. Presence of elevated level of indole-3-butyric acid (IBA) or 1-naphthalene acetic acid (NAA) with low cytokinin content in medium generated more in vitro rooting, though IBA was found to be more effective in rooting induction as compared to NAA. The in vitro protocol for asymbiotic seed germination developed from the present investigation can be used for rapid mass propagation of this highly important Dendrobium orchid species.

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Efficient micropropagation of Dendrobium sonia-28 for rapid PLBs proliferation

Cited by 9 publications

References 18 publications

In vitro pseudobulb based micropropagation for mass development of Cymbidium finlaysonianum Lindl.

In vitro pseudobulb based micropropagation for mass development of Cymbidium finlaysonianum Lindl.

Micropropagation of Tolumnia Orchids through Induction of Protocorm-like Bodies from Leaf Segments

Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

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