In vitro evaluation of Zeolite-nHA blended PCL/PLA nanofibers for dental tissue engineering

Mohandesnezhad, Sanam; Pilehvar-Soltanahmadi, Younes; Alizadeh, Effat; Goodarzi, Arash; Davaran, Soodabeh; Khatamian, M.; Zarghami, Nosratollah; Samiei, Mohammad; Aghazadeh, Mohammad; Akbarzadeh, Abolfazl

doi:10.1016/j.matchemphys.2020.123152

Cited by 77 publications

(39 citation statements)

References 32 publications

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“…The development of new technologies and devices may help in the regeneration of the oral soft tissues, thus allowing to obtain a physiologically stable masticatory apparatus and to reach an optimal aesthetic. An emerging and increasingly widespread innovation in dentistry is the use of nanotechnologies for oral tissue regeneration (Ahadian et al, 2020;Bonilla-Represa et al, 2020;Mohandesnezhad et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies proposed the use of nanofibers to regenerate (Gunn et al, 2010;Chanda et al, 2018) or deliver substances and compounds (Amler et al, 2014;Ahadian et al, 2020) in different tissues (Wang et al, 2017;Beznoska et al, 2019); however, only few preliminary published articles are available in oral fields (Bonilla-Represa et al, 2020;Mohandesnezhad et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Synthetic polymers, such as polycaprolactone (PCL), are largely used in several fields of regenerative medicine, including dentistry, to create bio-printed scaffolds for periodontal osseous defect treatment (Rasperini et al, 2015) and to produce nanofibers to stimulate human dental pulp-derived stem cells activity (Mohandesnezhad et al, 2020). Furthermore, PCL allows to produce miscible polyblend with natural polymers (Gunn et al, 2010) and substances.…”

Section: Introductionmentioning

confidence: 99%

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Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

et al. 2021

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Aim: The regeneration of small periodontal defects has been considered an important divide and challenging issue for dental practitioners. The aim of this preliminary in vitro study was to analyze the effects of polycaprolactone (PCL) nanofibers enriched with hyaluronic acid and vitamin E vs. nude nanofibers on gingival fibroblasts activity, an innovative graft for periodontal soft tissue regeneration purposes.Methods: Nanofibers were produced in PCL (NF) or PCL enriched with hyaluronic acid and vitamin E (NFE) by electrospinning technique. NF and NFE were stereologically and morphologically characterized by scanning electron microscope (SEM), and composition was analyzed by infrared spectroscopy. Human fibroblasts were obtained from one gingival tissue fragment (HGF) and then seeded on NF, NFE, and plastic (CT). Cell adhesion and morphology were evaluated using SEM at 24 h and cell viability after 24, 48, and 72 h by alamarBlue® assay. Gene expression for COL-I, LH2b, TIMP-1, PAX, and VNC was analyzed by real-time RT-PCR in samples run in triplicate and GAPDH was used as housekeeping gene. Slot blot analysis was performed and immunoreactive bands were revealed for MMP-1 and COL-I. YAP and p-YAP were analyzed by Western blot and membranes were reprobed by α-tubulin. Statistical analysis was performed.Results: IR spectrum revealed the presence of PCL in NF and PCL and vitamin E and hyaluronic acid in NFE. At 24 h, HGF adhered on NF and NFE conserving fibroblast like morphology. At 72 h from seeding, statistically significant differences were found in proliferation of HGF cultured on NF compared to NFE. Expression of genes (LH2b, TIMP-1, and MMP-1) and proteins (COL-I) related to collagen turnover revealed a reduction of COL-1 secretion in cells cultured on NF and NFE compared to CT; however, NFE stimulated cross-linked collagen deposition. Mechanosensor genes (PAX, VNC, and YAP) were upregulated in HGF on NF while they were decreased in cells grown on NFE.Conclusion: Preliminary data suggest that PCL-enriched nanofibers could represent a support to induce HGF proliferation, adhesion, collagen cross-linking, and to reduce collagen degradation, therefore favoring collagen deposition in gingival connective tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

et al. 2021

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“…There are different types of nanocarriers are functionally used in tissue engineering applications, including polymeric nanoparticles [129], gold nanoparticles [130], titanium oxide nanoparticles [131], dendrimers [132], liposomes [133] and micelle [134]. A schematic illustration of the different types of nanocarrier used for tissue engineering applications is shown in Figure 1 and different types of nanomaterial characterization techniques are illustrated in Figure 2.…”

Section: The Potential Role Of Nanocarriers In Tissue Engineeringmentioning

confidence: 99%

Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications

Kumar

Abrahamse

2020

IJMS

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Recent advancement in nanotechnology has provided a wide range of benefits in the biological sciences, especially in the field of tissue engineering and wound healing. Nanotechnology provides an easy process for designing nanocarrier-based biomaterials for the purpose and specific needs of tissue engineering applications. Naturally available medicinal compounds have unique clinical benefits, which can be incorporated into nanobiomaterials and enhance their applications in tissue engineering. The choice of using natural compounds in tissue engineering improves treatment modalities and can deal with side effects associated with synthetic drugs. In this review article, we focus on advances in the use of nanobiomaterials to deliver naturally available medicinal compounds for tissue engineering application, including the types of biomaterials, the potential role of nanocarriers, and the various effects of naturally available medicinal compounds incorporated scaffolds in tissue engineering.

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“…Other tissue engineering and commercial prospectus Apart from the above tissue engineering applications, the electrospun nanofibers have been extensively explored for their potentials in other engineering other tissues including cardiac [137], skeletal muscle [138], tendon/ligament [139], blood vessel [107], dental [140], vascular [141], urethral [142] and cornea [143]. Based on the extensive discussion made throughout the article, it has been well evidenced that the electrospun nanofibers have been vastly used in the fabrication of scaffolds by adopting several approaches.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Electrospun Nanofibers for Wound Dressing and Tissue Engineering Applications

Balusamy

Anitha

Uyar

2020

Hacettepe Journal of Biology and Chemistry

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E lektro-eğirme yöntemi ile üretilen nanolifler, son yıllarda nanofibröz yapı iskelelerinin geliştirilmesinde büyük ilgi gördü ve biyomimetik yapıları nedeniyle farklı biyomedikal uygulamalarda kullanıldı. Özellikle, elektro-eğirme yöntemi ile üretilen nanolifler doğal hücre dışı matris (ECM), birbirine bağlı gözenekler, geniş yüzey alanı, işlevselleştirme kolaylığı ve hücre adezyonunu, farklılaşmasını ve proliferasyon davranışını etkilemede büyük önem taşıyan mekanik performans dahil olmak üzere birçok faydalı özellik sergiler. Bugüne kadar, çok çeşitli yararlı özellikleri kabul eden bu nanolifler, yara örtüsü ve doku mühendisliği uygulamalarında kullanılmıştır. Bu derlemede, çeşitli malzemelerin ve yaklaşımların kullanımını gösteren birkaç temsili örneklerle bu alanlarda çeşitli çabaların yapıldığını özetlemektedir. Ayrıca, klinik faz transferine ilişkin gelecekteki yön endişeleri tartışılmıştır. Anahtar Kelimeler Elektro-eğirme, nanolif, yara örtüsü, doku mühendisliği.

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In vitro evaluation of Zeolite-nHA blended PCL/PLA nanofibers for dental tissue engineering

Cited by 77 publications

References 32 publications

Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications

Electrospun Nanofibers for Wound Dressing and Tissue Engineering Applications

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