In Vitro Evaluation of the Effect of a Not Cross-Linked Hyaluronic Acid Hydrogel on Human Keratinocytes for Mesotherapy

Zerbinati, Nicola; Sommatis, Sabrina; Maccario, Cristina; Capillo, Maria Chiara; Francesco, S. Di; Rauso, Raffaele; Protasoni, Marina; D’Este, Edoardo; Gasperina, Daniela Dalla; Mocchi, Roberto

doi:10.3390/gels7010015

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…The experiments were carried out focusing on the possible protective effect in response to stress-inducing conditions triggered by environmental damage, as the oxidative stress related to ultraviolet (UV) radiation and the ability to promote VEGF release. Our results consolidate the data recently published on human keratinocytes [ 17 ], giving the product a scientific support to be a good candidate for biorevitalization of the hair follicle and future clinical investigations.…”

Section: Introductionsupporting

confidence: 88%

“…The tested product is characterized by a noncrosslinked HA added with several amino acids, as lysine, proline, leucine, isoleucine, valine, serine, and alanine. As recently published [ 17 ], the product shows high biocompatibility and the ability to significantly reduce the expression of the inflammatory marker IL-8 and the vascular growth factor in human keratinocytes. Considering the constant interaction between keratinocytes and dermal papilla cells in the hair structure, our experiments were carried out using human follicle dermal papilla cells (HFDPCs).…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Hair Growth Promoting Effect of a Noncrosslinked Hyaluronic Acid in Human Dermal Papilla Cells

Zerbinati

Sommatis

Maccario

et al. 2021

BioMed Research International

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Dermal papilla cells (DPCs) are a source of nutrients and growth factors, which support the proliferation and growth of keratinocytes as well as promoting the induction of new hair follicles and maintenance of hair growth. The protection from reactive oxygen species (ROS) and the promotion of angiogenesis are considered two of the basal mechanisms to preserve the growth of the hair follicle. In this study, a noncrosslinked hyaluronic acid (HA) filler (HYDRO DELUXE BIO, Matex Lab S.p.A.) containing several amino acids was tested with in vitro assays on human follicle dermal papilla cells (HFDPCs). The experiments were carried out to investigate the possible protection against oxidative stress and the ability to increase the vascular endothelial growth factor (VEGF) release. The results demonstrated the restoration of cell viability against UVB-induced cytotoxicity and an increase in the VEGF secretion. These data demonstrate the capability of the product to modulate human dermal papilla cells, suggesting a future use in mesotherapy, a minimally invasive local intradermal therapy (LIT), after further clinical investigations.

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Section: Introductionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

In Vitro Hair Growth Promoting Effect of a Noncrosslinked Hyaluronic Acid in Human Dermal Papilla Cells

Zerbinati

Sommatis

Maccario

et al. 2021

BioMed Research International

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“…Softening rough nail plates and minimizing distal breakage would positively influence wholescale nail resistance and appearance. Because HA has a good surface adherence property, it would enhance the deep moisturizing of nail compartments (Zerbinati et al, 2021b). The adhesion property refers to the ionic interaction between the positively charged acid and the nail surface.…”

Section: Nail Care Productsmentioning

confidence: 99%

A review of the cosmetic use and potentially therapeutic importance of hyaluronic acid

Al-Halaseh¹,

Tarawneh²,

Al-Jawabri³

et al. 2022

j app pharm sci

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Hyaluronic acid (HA) is an endogenous substance detected and isolated from various tissues and biological fluids. Owing to its physicochemical properties, HA contributes to many biological processes at intra-and extracellular levels, such as skin hydration, joints lubrication, and wound healing, besides its anti-inflammatory and antioxidant effects. The current review highlights the employment of exogenous HA in many aesthetic and dermatological aspects. Also, it aims to clarify the skin rejuvenating activity by compensating for the biological loss of HA with advanced age. Other positive impacts of HA on hair, nail, and overall health status were also revised. Cosmetics and cosmeceuticals that contain HA, among other active ingredients, are commercially available in different dosage forms such as gels, creams, intradermal injections, and fillers. Aesthetic enhancement is achieved via soft tissue augmentation, skin hydration level increasing, grooves refilling, and collagen and elastin biosynthesis stimulation. HA contributes to various therapeutic approaches such as gingivitis, stomatitis, ulceration, and osteoarthritis. Additionally, it has beneficial uses in dentistry due to its antioxidant and anti-inflammatory properties. The recent implication of HA following the claims that it has a rejuvenating power for the skin and many therapeutic benefits has to be thoroughly investigated and delivered by a trained expert to avoid undesirable effects.

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“…The introduction of HA-based formulations as multiple micro-injections promotes skin hydration as well as keratinocyte differentiation to provide an optimal environment for healthy skin homeostasis and protect against extrinsic aging-related risk factors [ 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Combined Bipolar Radiofrequency and Non-Crosslinked Hyaluronic Acid Mesotherapy Protocol to Improve Skin Appearance and Epidermal Barrier Function: A Pilot Study

Korzekwa¹,

Łukasik²,

Zerbinati

2023

Pharmaceuticals

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Background: Age-associated changes in epidermal hydration, pigmentation, thickness and cell renewal influence skin appearance and can lead to laxity, dryness and poor skin tone. The aim of this pilot study was to assess the synergistic effects of a new bipolar radiofrequency plus non-crosslinked hyaluronic acid (HA) mesotherapy protocol compared with radiofrequency alone on skin appearance and markers of epidermal function. Methods: This prospective, single-center, split-face pilot study recruited women aged 25–65 years with dryness and laxity of the facial skin defined by a trans-epidermal water loss (TEWL) value of ≥26 g/m2/h. Subjects were treated with a bipolar radiofrequency device on both sides of the face. This was immediately followed by needle hyaluronic acid (HA) treatment on one side of the face with 2.5 mL of a non-crosslinked HA. Photographic documentation, analysis of epidermal barrier function parameters, and high frequency (HF) ultrasound analysis were performed prior to treatment and at 28 days. Results: Twenty female subjects with a mean age of 46 (range 29 to 54) years and dry and lax facial skin were included. TEWL was reduced and skin hydration improved to a greater extent with the combined radiofrequency plus mesotherapy protocol compared with radiofrequency alone (−5.8% vs. +3.9% and +23.1% vs. +1.0%, respectively). The combined protocol was also associated with greater improvements in melanin (−7.5% vs. −1.5%) and erythema values (−7.2% vs. +3.0%), respectively. Ultrasound measures of epidermal thickness and epidermal density were greater after the combined protocol compared with radiofrequency alone (12.0% vs. 5.6% and 57.7% vs. 7.1%, respectively). Both treatments were well-tolerated. Conclusions: The combined bipolar radiofrequency and HA mesotherapy protocol provided greater improvements in skin hydration, firmness and tone compared with radiofrequency alone. The combination treatment was also associated with greater epidermal thickness and density and increased keratinocyte differentiation suggesting a synergistic effect of both treatments on epidermal homeostasis and barrier function. Both treatments were well-tolerated and led to improvements in facial appearance.

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In Vitro Evaluation of the Effect of a Not Cross-Linked Hyaluronic Acid Hydrogel on Human Keratinocytes for Mesotherapy

Cited by 6 publications

References 23 publications

In Vitro Hair Growth Promoting Effect of a Noncrosslinked Hyaluronic Acid in Human Dermal Papilla Cells

In Vitro Hair Growth Promoting Effect of a Noncrosslinked Hyaluronic Acid in Human Dermal Papilla Cells

A review of the cosmetic use and potentially therapeutic importance of hyaluronic acid

Combined Bipolar Radiofrequency and Non-Crosslinked Hyaluronic Acid Mesotherapy Protocol to Improve Skin Appearance and Epidermal Barrier Function: A Pilot Study

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