In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines

Abstract

Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the IC 50 values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and 751.9 µg/mL, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

Moringa oleifera Seeds and Oil: Characteristics and Uses for Human Health

“…However, recent study results found that the ethanol extract of M. oleifera seeds had no significant effect in inhibiting the proliferation of breast and colorectal tumour cells [63]. Nevertheless, a recent study observed a cytotoxic effect of M. oleifera oil in several cancer cell lines [64]. …”

Lagerstroemia Speciosa (L.) Pers Leaf Extract Attenuates Lung Tumorigenesis via Alleviating Oxidative Stress, Inflammation and Apoptosis

“…The in vitro cytotoxic effects of LLE extract were evaluated using the standard MTT assay [24,25]. The method depends on the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial dehydrogenases in living cells.…”

Aktivitas Sitotoksik Ekstrak Etanol, Fraksi Etil Asetat dan n-heksana Daun Kelor (Moringa oleifera) Terhadap Sel Kanker Payudara T47D

“…Perkembangan yang pesat dalam bidang isolasi dan identifikasi senyawa fitokimia meningkatkan trend penelitian selama dua dekade terakhir ini terhadap pengobatan yang menggunakan tanaman herbal sebagai sumber potensial untuk agen antikanker (Awodele et al, 2012;Elsayed et al, 2015). Studi farmakologis menunjukkan bahwa ekstrak herbal terdiri dari nutrisi penting, beberapa kandungan senyawa yang memiliki aktivitas antitumor, antioksidan dan antimutasi dengan berbagai target yang saling bekerja secara sinergis (Kalappurayil and Joseph, 2017;Leone et al, 2015).…”

“…Hasil penelitian ini menunjukkan bahwa ekstrak etanol, fraksi etil asetat dan nheksan daun M. oleifera bersifat sitotoksik terhadap sel kanker payudara T47D dengan nilai IC50 yang berbeda-beda. Berdasarkan nilai IC50-nya, maka fraksi etil asetat merupakan fraksi yang paling sitotoksik.Sejumlah penelitian terhadap sel kanker yang berbeda sudah melaporkan aktivitas sitotoksik dari ekstrak M. oleifera Elsayed et al, 2015,. melaporkan nilai IC50 ekstrak M Tiloke et al, 2013)…”