In vitro effects of sodium fluoride and sodium dichromate on dynamic properties of human erythrocyte membrane

Korkmaz, Özden

doi:10.1016/s0301-4622(99)00129-5

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…In vertebrates with fluorosis, hypochromic anemia, alterations in erythrocyte structure, and other hematologic alterations have been observed [3–9]. Moreover, in vitro experiments showed that alterations in metabolism as well as structural damage occur in erythrocytes exposed to fluoride compounds [10, 29]. …”

Section: Discussionmentioning

confidence: 99%

“…This is due to the structural similarity between NaF and some of the known substrates for oxidoreductases and ion cotransporters present in cell membranes. Indirectly, another possible effect of NaF is inducing protein denaturation because of the effect of oxidative stress it causes in cells [1–3, 6, 10–15, 18, 29, 30, 38]. …”

Section: Discussionmentioning

confidence: 99%

“…Additionally, several biochemical and structural alterations have been described in erythrocytes exposed to fluoride compounds in vitro [4–10]. …”

Section: Introductionmentioning

confidence: 99%

“…Oxidative stress in response to fluoride has been observed in different types of cells and tissues experimentally exposed in vitro or in vivo, as well as in the tissues of animals and people living in endemically fluoridated areas [10–15]. …”

Section: Introductionmentioning

confidence: 99%

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In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes

Gutiérrez‐Salinas

García-Ortiz

Morales-González

et al. 2013

The Scientific World Journal

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The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 μg/mL) or NaF (100 μg/mL) + vitamin E (1, 2.5, 5 and 10 μg/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Additionally, several biochemical and structural alterations have been described in erythrocytes exposed to fluoride compounds in vitro [4–10]. …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes

Gutiérrez‐Salinas

García-Ortiz

Morales-González

et al. 2013

The Scientific World Journal

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“…Following ingestion, fluoride accumulates in bones and teeth, disperses towards cardiac muscle, liver, brain, kidney and other soft tissues (Shivashankara et al 2000;Vani & Reddy 2000) and cause metabolic disturbances (Chirumari & Reddy 2007). In vivo studies confirm that the fluoride ion is strongly reactive due to its high negative charge density and alter the distribution of electrons, including enzymes and membrane transport proteins, thereby affecting the metabolites of many cells, tissues and organs (Korkmaz 2000). In advanced stages of fluorosis, neurological manifestations such as paralysis of limbs, vertigo, spasticity in extremities and impaired mental acuity are observed in humans (Inkielewicz & Czanowski 2008;Inkielewicz & Krechniak 2004;Mullenix et al 1995).…”

Section: Introductionmentioning

confidence: 97%

Effect of maternal exposure of fluoride on oxidative stress markers and amelioration by selected antioxidants in developing central nervous system of rats

Basha

Narayanaswamy

2010

Biologia

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Fluoride has been implicated as a pathologic mediator of fluorosis. Interestingly neuronal destruction, synaptic injury occurs by a mechanism involving oxidative stress, however, its effects in developmental stages of life, during maternal fluoride exposure and amelioration are not elucidated. In the present study, pregnant Wistar albino rats were exposed to 50 and 150 ppm fluoride in drinking water during gestation and post gestation. After parturition the pups born to the experimental animals were administered daily with selected antioxidants for 21 consecutive days. Fluoride administration substantially enhanced fluoride accumulation, lipid peroxidation and decreased the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione levels in discrete regions of central nervous system. The results significantly (P < 0.05) demonstrated the effect of fluoride through exacerbated oxidative damage and disrupted antioxidant homeostasis, leading to altered neuronal integrity. The administration of antioxidants vitamin E, vitamin C, selenium and zinc produced a promising accost and timely intervention to the aggravated impairment during highly vulnerable early stage of life.

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The Role of Calcium in Ameliorating the Oxidative Stress of Fluoride in Rats

Mohamed

2015

Biol Trace Elem Res

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The present study was carried out to investigate the effects of fluoride toxicity on some biochemical, hormonal, and histological parameters of female rats and the protective role of calcium against such effects. Adult female albino rats were divided into five groups; control group received distilled water for 60 days, calcium group received calcium carbonate with dose of 50 mg/kg three times per week for 60 days, fluoride group received sodium fluoride with dose of 20 mg/kg three times per week for 60 days, calcium + fluoride group received calcium carbonate (50 mg/kg) then after 2 h received sodium fluoride (20 mg/kg) three times per week for 60 days, and fluoride + calcium group received sodium fluoride (20 mg/kg) three times per week for 30 days then received calcium carbonate (50 mg/kg) three times per week for another 30 days. The results showed that the levels of thiobarbituric acid reactive substances, urea, creatinine, alkaline phosphatase, triiodothyronine, thyroxine, parathormone, phosphorous, magnesium, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma glutamyl transferase were significantly increased in rats treated with fluoride while serum estradiol, calcium, and organ glutathione were significantly decreased. The histological examination of the femur bone revealed that fluoride treatment induced thinning of bone trabeculae with wilding of marrow space, demineralization, and loss of trabeculae interconnections. Also, the histological examination of hepatic and renal tissues of fluoride-treated rats showed some damages in these tissues while administration of calcium carbonate for 30 or 60 days during fluoride treatment minimized such damages. It could be concluded that administration of calcium to female rats can ameliorate the hazardous effects of fluoride observed in the biochemical, hormonal, and histological parameters.

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In vitro effects of sodium fluoride and sodium dichromate on dynamic properties of human erythrocyte membrane

Cited by 8 publications

References 23 publications

In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes

In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes

Effect of maternal exposure of fluoride on oxidative stress markers and amelioration by selected antioxidants in developing central nervous system of rats

The Role of Calcium in Ameliorating the Oxidative Stress of Fluoride in Rats

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